FastQCFastQC Report
Fri 10 Feb 2017
ERR1632300.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632300.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences230326
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT5350.23227946475864644No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA5110.22185945138629595No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG4760.20666359855161814No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG4640.2014535918654429No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG4380.19016524404539653No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC4230.18365273568767745No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC4120.17887689622535016No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC4090.17757439455380633No Hit
CTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGATGAATACC3990.173232722315327No Hit
GGATCACTGAGTGGGTCTGCCTGGGAAGCTGAGAATGATCTGG3710.16107604004758472No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT3680.1597735383760409No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT3560.15456353168986567No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC3430.14891935777984247No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3410.14805102333214662No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA3390.14718268888445074No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC3330.1445776855413631No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT3250.14110434775057962No Hit
GTCTTGAAGGGAACGTTGCCAGCTGCCTTGTACCAGCATTACA3180.13806517718364406No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC3170.1376310099597961No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA3090.13415767216901262No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA3030.131552668825925No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA3020.13111850160207705No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC2990.12981599993053325No Hit
GTGTTCATCTCATCAGAGAAAGAACCATCAGCATGTCTGCGGC2870.12460599324435799No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC2850.12373765879666213No Hit
GATGAACACCATTCTTGATAATCTTGCCGCCAGGGACTTTATA2850.12373765879666213No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT2700.11722515043894306No Hit
CATCAAAAGTTCCCAAAGAGGGCTTGCTCTCTCTTCACCTGCT2670.11592264876739924No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG2600.11288347820046368No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2570.11158097652891986No Hit
GCCTTGTACCAGCATTACAAATAATCCAGCCACAAAGTAAATG2540.11027847485737606No Hit
GTGAAAGGCCGAGGAAGGCGAGATTTCCCAGAAGAGGTCGCCA2450.10637096984274462No Hit
ACACAACACTAAAAGAAAATTTATTTATTGGCATGCAAAGCAA2420.10506846817120082No Hit
GTGCTAGATTCTGTTATTTTCTTCTTATTTTGGATGAAGTACC2420.10506846817120082No Hit
TCATCAACCACTGCACAAAATCTTGGGCACGCCTGGAGTCCAG2420.10506846817120082No Hit
CTTCAGCATGTCTCTCAAATTCATCGTGACGTTTGGCAATGTT2390.103765966499657No Hit
GCCTGGGAAGCTGAGAATGATCTGGATTTCTCCTCTGTGTCTT2340.10159513038041731No Hit
ATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAGGGCAC2330.10116096315656938No Hit
TTCCCAGGCAGACCCACTCAGTGATCCTGATCAGATGAACGAG2320.10072679593272145No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT352.380084E-531.71428737
GCCGTCT352.380084E-531.71428736
GGTATCA1300.031.3076921
TATGCCG405.919839E-527.7533
CTACTCT400.001926526823.1254
TTATGCT400.001926526823.1254
CTAGCAC603.709658E-521.5833343
TAGATTC806.9105226E-720.81255
TCAGGAC450.00381666420.5555553
CTTATAC1101.7334969E-920.18181837
ATGCCGT555.125952E-420.18181834
TTTAGTA500.007018229518.528
CTCGTAT609.2062773E-418.529
TAGCACT701.21383375E-418.54
TCGTATG609.2062773E-418.530
GCTAGAT902.1370524E-618.53
CTTTCAT609.2062773E-418.59
CTGATAT701.21383375E-418.525
TGCCGTC609.2062773E-418.535
GTATCAA2250.018.0888882
Produced by FastQC (version 0.11.5)