##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632300.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	230326
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.430229327127634	33.0	31.0	34.0	31.0	34.0
2	32.624189192709466	34.0	31.0	34.0	31.0	34.0
3	32.701605550393786	34.0	31.0	34.0	31.0	34.0
4	36.211443779686185	37.0	37.0	37.0	35.0	37.0
5	35.99234563184356	37.0	35.0	37.0	35.0	37.0
6	36.01022897979386	37.0	35.0	37.0	35.0	37.0
7	35.993587350103766	37.0	35.0	37.0	35.0	37.0
8	35.973407257539314	37.0	35.0	37.0	35.0	37.0
9	37.69653881889148	39.0	37.0	39.0	35.0	39.0
10	37.64483384420343	39.0	37.0	39.0	35.0	39.0
11	37.706472564973126	39.0	37.0	39.0	35.0	39.0
12	37.67654541823329	39.0	37.0	39.0	35.0	39.0
13	37.689778835216174	39.0	37.0	39.0	35.0	39.0
14	39.01110165591379	40.0	38.0	41.0	36.0	41.0
15	39.02994885510103	40.0	38.0	41.0	36.0	41.0
16	39.009056728289465	40.0	38.0	41.0	36.0	41.0
17	38.990826046560095	40.0	38.0	41.0	36.0	41.0
18	38.98288512803592	40.0	38.0	41.0	36.0	41.0
19	39.04343408907375	40.0	38.0	41.0	36.0	41.0
20	39.02103974366767	40.0	38.0	41.0	36.0	41.0
21	39.001424068494224	40.0	38.0	41.0	36.0	41.0
22	38.9757300521869	40.0	38.0	41.0	36.0	41.0
23	38.940028481369886	40.0	38.0	41.0	36.0	41.0
24	38.908360324062414	40.0	38.0	41.0	36.0	41.0
25	38.855118397401945	40.0	38.0	41.0	35.0	41.0
26	38.77572223717687	40.0	38.0	41.0	35.0	41.0
27	38.698453496348655	40.0	38.0	41.0	35.0	41.0
28	38.652223370353326	40.0	38.0	41.0	35.0	41.0
29	38.596376440349765	40.0	38.0	41.0	35.0	41.0
30	38.55247345067426	40.0	38.0	41.0	35.0	41.0
31	38.45936628952007	40.0	38.0	41.0	34.0	41.0
32	38.376722558460614	40.0	38.0	41.0	34.0	41.0
33	38.32094943688511	40.0	38.0	41.0	34.0	41.0
34	38.24207861900089	40.0	38.0	41.0	34.0	41.0
35	38.23200159773538	40.0	38.0	41.0	34.0	41.0
36	38.20183565902243	40.0	38.0	41.0	34.0	41.0
37	38.153391280185474	40.0	38.0	41.0	34.0	41.0
38	38.12519646066879	40.0	38.0	41.0	34.0	41.0
39	38.088387763430966	40.0	38.0	41.0	34.0	41.0
40	38.04736764412181	40.0	38.0	41.0	34.0	41.0
41	38.02328004654272	40.0	38.0	41.0	33.0	41.0
42	37.98724850863559	40.0	38.0	41.0	33.0	41.0
43	37.16695466425848	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	0.0
14	1.0
15	0.0
16	0.0
17	1.0
18	6.0
19	7.0
20	15.0
21	28.0
22	51.0
23	109.0
24	161.0
25	266.0
26	439.0
27	609.0
28	940.0
29	1376.0
30	1962.0
31	2585.0
32	3536.0
33	4759.0
34	6870.0
35	10261.0
36	16867.0
37	31391.0
38	71717.0
39	76367.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	40.940232539965095	20.122782490904196	13.771350173232722	25.16563479589799
2	18.228945060479496	22.063944148728325	36.13356720474458	23.573543586047602
3	19.96648229031894	23.418545887133888	31.306061842779364	25.308909979767808
4	15.872719536656737	16.63641968340526	33.84897927285673	33.641881507081266
5	14.823771523840124	36.581193612531806	34.105137934927015	14.489896928701059
6	33.623212316455806	34.68344867709247	16.106735670310776	15.586603336140946
7	27.354705938539286	30.799388692548824	21.907643948143065	19.938261420768825
8	24.731033404826203	35.01558660333614	20.94292437675295	19.310455615084706
9	24.830891866311227	15.887481222267569	20.85001259084949	38.43161432057171
10	16.688953917490863	28.25560292802376	34.078653734272294	20.97678942021309
11	34.09254708543543	22.723444161753342	22.505492215381675	20.678516537429555
12	20.38936116634683	26.650486701457933	28.906419596571816	24.05373253562342
13	28.93637713501732	20.882575132638088	25.479103531516202	24.70194420082839
14	22.22502018877591	22.45035297795299	24.51481812734993	30.80980870592117
15	24.673723331278275	29.14304073356894	23.057318756892407	23.12591717826038
16	24.58471905038945	27.77715064734333	24.03940501723644	23.598725285030785
17	23.255299010967065	27.754139784479392	25.48518187265007	23.505379331903477
18	21.775657112093295	26.761633510763005	27.494941951842172	23.967767425301528
19	24.15272266266075	26.87625365785886	27.234875784757257	21.736147894723132
20	23.762406328421452	26.030930073026926	27.767164801194827	22.43949879735679
21	23.065567934145513	27.52012365082535	25.835554822295354	23.57875359273378
22	23.312174917291145	26.10821183887186	26.282312895634885	24.297300348202114
23	22.905360228545625	27.315630888392974	26.235857002683154	23.543151880378247
24	25.442199317489123	26.64484252754791	25.635837899325303	22.277120255637662
25	24.204388562298654	25.934979116556534	26.523709872094337	23.336922449050476
26	24.179641030539322	26.08910848102255	25.88765488915711	23.84359559928102
27	23.43113673662548	26.099528494394903	26.146418554570477	24.322916214409144
28	22.733864175125692	26.44078393233938	26.815036079296302	24.010315813238627
29	23.442425084445524	27.194498232939402	26.188098608059878	23.1749780745552
30	22.910136068007954	28.0567543395014	25.837291491190744	23.1958181012999
31	23.887446488889662	26.13556437397428	26.94572041367453	23.03126872346153
32	22.74862586073652	26.280576226739488	26.877990326754254	24.092807585769734
33	23.33605411460278	25.987513350642132	26.775526861926142	23.900905672828948
34	23.316082422305776	26.356989658136726	26.360028828703662	23.966899090853833
35	23.577885258286084	26.100830996066442	26.215451143162298	24.105832602485172
36	23.372958328629856	26.31791460799041	26.78377603917925	23.525351024200482
37	24.237819438534945	24.283841164262828	26.81156274150552	24.666776655696708
38	22.880178529562446	25.67230794612853	27.992931757595755	23.454581766713268
39	22.46641716523536	24.82828686296814	28.932469630002693	23.772826341793802
40	22.570183131735018	23.905247345067426	29.58719380356538	23.937375719632172
41	21.834703854536613	25.018452107013537	29.30976094752655	23.8370830909233
42	22.42907878398444	23.93346821461754	28.84476785078541	24.79268515061261
43	21.40878580794179	24.78400180613565	28.949836318956606	24.85737606696595
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	6.5
2	11.0
3	35.5
4	60.0
5	60.0
6	75.5
7	91.0
8	95.0
9	99.0
10	156.0
11	213.0
12	213.0
13	346.0
14	479.0
15	756.0
16	1033.0
17	1103.0
18	1173.0
19	1173.0
20	1402.0
21	1631.0
22	2032.0
23	2433.0
24	2736.0
25	3039.0
26	3039.0
27	3464.0
28	3889.0
29	5497.0
30	7105.0
31	7368.5
32	7632.0
33	7632.0
34	8438.0
35	9244.0
36	9656.5
37	10069.0
38	12431.5
39	14794.0
40	14794.0
41	16019.5
42	17245.0
43	15947.0
44	14649.0
45	15736.0
46	16823.0
47	16823.0
48	17612.5
49	18402.0
50	19548.0
51	20694.0
52	21181.0
53	21668.0
54	21668.0
55	17772.0
56	13876.0
57	12254.0
58	10632.0
59	9396.0
60	8160.0
61	8160.0
62	7561.5
63	6963.0
64	6192.5
65	5422.0
66	4720.0
67	4018.0
68	4018.0
69	3500.0
70	2982.0
71	2568.5
72	2155.0
73	1798.0
74	1441.0
75	1441.0
76	1149.0
77	857.0
78	710.5
79	564.0
80	465.0
81	366.0
82	366.0
83	290.5
84	215.0
85	164.5
86	114.0
87	83.5
88	53.0
89	53.0
90	36.0
91	19.0
92	13.0
93	7.0
94	5.0
95	3.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	230326.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.17821063505068
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.42107441598947	46.136534454549896
2	6.125846876315081	6.392714572608712
3	1.637966805766464	2.563985310135112
4	0.792428728318245	1.6539005239781899
5	0.5141408869875279	1.341347574866351
6	0.3790220711730716	1.186601607900102
7	0.2535566483762128	0.9261092544824059
8	0.2038112562446561	0.8507605326502364
9	0.16304228688155978	0.7656529308593749
>10	1.0895816413663872	12.024074789159817
>50	0.24058262697791202	8.930906812349457
>100	0.17695746943003446	16.68482416328166
>500	0.0019882861733711735	0.5425874731786916
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	535	0.23227946475864644	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	511	0.22185945138629595	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	476	0.20666359855161814	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	464	0.2014535918654429	No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG	438	0.19016524404539653	No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC	423	0.18365273568767745	No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC	412	0.17887689622535016	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	409	0.17757439455380633	No Hit
CTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGATGAATACC	399	0.173232722315327	No Hit
GGATCACTGAGTGGGTCTGCCTGGGAAGCTGAGAATGATCTGG	371	0.16107604004758472	No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT	368	0.1597735383760409	No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT	356	0.15456353168986567	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	343	0.14891935777984247	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	341	0.14805102333214662	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	339	0.14718268888445074	No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC	333	0.1445776855413631	No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT	325	0.14110434775057962	No Hit
GTCTTGAAGGGAACGTTGCCAGCTGCCTTGTACCAGCATTACA	318	0.13806517718364406	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	317	0.1376310099597961	No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA	309	0.13415767216901262	No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA	303	0.131552668825925	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	302	0.13111850160207705	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	299	0.12981599993053325	No Hit
GTGTTCATCTCATCAGAGAAAGAACCATCAGCATGTCTGCGGC	287	0.12460599324435799	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	285	0.12373765879666213	No Hit
GATGAACACCATTCTTGATAATCTTGCCGCCAGGGACTTTATA	285	0.12373765879666213	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	270	0.11722515043894306	No Hit
CATCAAAAGTTCCCAAAGAGGGCTTGCTCTCTCTTCACCTGCT	267	0.11592264876739924	No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG	260	0.11288347820046368	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	257	0.11158097652891986	No Hit
GCCTTGTACCAGCATTACAAATAATCCAGCCACAAAGTAAATG	254	0.11027847485737606	No Hit
GTGAAAGGCCGAGGAAGGCGAGATTTCCCAGAAGAGGTCGCCA	245	0.10637096984274462	No Hit
ACACAACACTAAAAGAAAATTTATTTATTGGCATGCAAAGCAA	242	0.10506846817120082	No Hit
GTGCTAGATTCTGTTATTTTCTTCTTATTTTGGATGAAGTACC	242	0.10506846817120082	No Hit
TCATCAACCACTGCACAAAATCTTGGGCACGCCTGGAGTCCAG	242	0.10506846817120082	No Hit
CTTCAGCATGTCTCTCAAATTCATCGTGACGTTTGGCAATGTT	239	0.103765966499657	No Hit
GCCTGGGAAGCTGAGAATGATCTGGATTTCTCCTCTGTGTCTT	234	0.10159513038041731	No Hit
ATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAGGGCAC	233	0.10116096315656938	No Hit
TTCCCAGGCAGACCCACTCAGTGATCCTGATCAGATGAACGAG	232	0.10072679593272145	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	4.3416722384793726E-4	0.0
24	0.0	0.0	0.0	4.3416722384793726E-4	0.0
25	0.0	0.0	0.0	0.0021708361192396865	0.0
26	0.0	0.0	0.0	0.005210006686175247	0.0
27	0.0	0.0	0.0	0.018235023401613367	0.0
28	0.0	0.0	0.0	0.05861257521947153	0.0
29	0.0	0.0	0.0	0.10897597318583226	0.0
30	0.0	0.0	0.0	0.17062771897223936	0.0
31	0.0	0.0	0.0	0.3647004680322673	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTCTT	35	2.380084E-5	31.714287	37
GCCGTCT	35	2.380084E-5	31.714287	36
GGTATCA	130	0.0	31.307692	1
TATGCCG	40	5.919839E-5	27.75	33
CTACTCT	40	0.0019265268	23.125	4
TTATGCT	40	0.0019265268	23.125	4
CTAGCAC	60	3.709658E-5	21.583334	3
TAGATTC	80	6.9105226E-7	20.8125	5
TCAGGAC	45	0.003816664	20.555555	3
CTTATAC	110	1.7334969E-9	20.181818	37
ATGCCGT	55	5.125952E-4	20.181818	34
TTTAGTA	50	0.0070182295	18.5	28
CTCGTAT	60	9.2062773E-4	18.5	29
TAGCACT	70	1.21383375E-4	18.5	4
TCGTATG	60	9.2062773E-4	18.5	30
GCTAGAT	90	2.1370524E-6	18.5	3
CTTTCAT	60	9.2062773E-4	18.5	9
CTGATAT	70	1.21383375E-4	18.5	25
TGCCGTC	60	9.2062773E-4	18.5	35
GTATCAA	225	0.0	18.088888	2
>>END_MODULE