FastQCFastQC Report
Fri 10 Feb 2017
ERR1632299.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632299.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198525
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA2600.1309658733157033No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG2570.12945472862359905No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG2500.1259287243420224No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT2360.11887671577886917No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC2350.11837300088150106No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG2160.10880241783150736No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC2140.10779498803677118No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC2120.10678755824203501No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC2020.10175040926835412No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGAGT200.001838270237.01
GGTATCA1150.028.9565221
GCGGTTC400.00192559423.12500211
CTTATAC1254.0563464E-1019.2437
GTATCAA1900.018.52
TAGGACT500.007014896318.54
TAATGCA500.007014896318.54
TCTTATA2000.016.65000237
AGGACAC1352.1871529E-816.4444435
AGTTCCC957.012761E-515.57894825
ACATCAA957.012761E-515.57894817
TAGGACA957.012761E-515.5789484
CAAAAGT1001.0861438E-414.821
AACTTGG1001.0861438E-414.828
TATCAAC2550.014.5098043
TTAGGAC1152.1915841E-514.4782613
CACATCA1152.1915841E-514.47826116
GCTTAGG1152.1915841E-514.4782611
CCATTGT908.231579E-414.38888916
ATCAACG2353.6379788E-1214.1702144
Produced by FastQC (version 0.11.5)