##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632299.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	198525
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.29783150736683	31.0	31.0	34.0	30.0	34.0
2	31.500304747512907	31.0	31.0	34.0	30.0	34.0
3	31.58173529782143	31.0	31.0	34.0	30.0	34.0
4	35.41895982873694	37.0	35.0	37.0	33.0	37.0
5	34.48389371615666	35.0	35.0	37.0	30.0	37.0
6	34.44122906434958	35.0	35.0	37.0	30.0	37.0
7	34.43012215086261	35.0	35.0	37.0	30.0	37.0
8	34.36056416068505	35.0	35.0	37.0	30.0	37.0
9	35.933610376526886	37.0	35.0	39.0	32.0	39.0
10	35.63972799395542	37.0	35.0	39.0	30.0	39.0
11	35.819428283591485	37.0	35.0	39.0	30.0	39.0
12	35.73975569827478	37.0	35.0	39.0	30.0	39.0
13	35.85042689837552	37.0	35.0	39.0	30.0	39.0
14	36.79843848381816	38.0	36.0	40.0	31.0	41.0
15	36.73015992947992	38.0	36.0	40.0	31.0	41.0
16	36.70216849263317	38.0	36.0	40.0	31.0	41.0
17	36.665397305125296	38.0	36.0	40.0	31.0	41.0
18	36.625573605339376	38.0	36.0	40.0	31.0	41.0
19	36.668515300340005	38.0	36.0	40.0	31.0	41.0
20	36.64251857448684	38.0	36.0	40.0	31.0	41.0
21	36.59618687822692	38.0	35.0	40.0	31.0	41.0
22	36.55045460269488	38.0	35.0	40.0	31.0	41.0
23	36.50163455484196	38.0	35.0	40.0	30.0	41.0
24	36.39445913612895	38.0	35.0	40.0	30.0	41.0
25	36.29236116358141	38.0	35.0	40.0	30.0	41.0
26	36.118121143432816	38.0	35.0	40.0	30.0	41.0
27	36.00346555849389	38.0	34.0	40.0	29.0	41.0
28	35.90711497292532	38.0	34.0	40.0	29.0	41.0
29	35.79549175166856	38.0	34.0	40.0	29.0	41.0
30	35.67176426142803	38.0	34.0	40.0	28.0	41.0
31	35.55437854174537	38.0	34.0	40.0	28.0	41.0
32	35.413021030096964	38.0	34.0	40.0	27.0	41.0
33	35.318514041052765	38.0	34.0	40.0	27.0	41.0
34	35.23532552575242	38.0	34.0	40.0	27.0	41.0
35	35.21305629013978	38.0	34.0	40.0	27.0	41.0
36	35.13273391260547	38.0	33.0	40.0	27.0	40.0
37	35.07287747135121	38.0	33.0	40.0	27.0	40.0
38	34.98676237249717	38.0	33.0	40.0	27.0	40.0
39	34.94173026067246	37.0	33.0	40.0	26.0	40.0
40	34.88613021030097	37.0	33.0	39.0	26.0	40.0
41	34.814139277169126	37.0	33.0	39.0	26.0	40.0
42	34.78197708097217	37.0	33.0	39.0	26.0	40.0
43	33.799788439743104	36.0	31.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	2.0
15	1.0
16	5.0
17	17.0
18	31.0
19	60.0
20	112.0
21	152.0
22	272.0
23	484.0
24	748.0
25	1156.0
26	1617.0
27	2270.0
28	3230.0
29	4442.0
30	5888.0
31	7759.0
32	10334.0
33	13834.0
34	18397.0
35	23982.0
36	29519.0
37	33819.0
38	30419.0
39	9974.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.24568694119128	19.44641732779247	13.402845989170128	25.905049741846113
2	19.969777106157917	21.24669437098602	33.73126810225413	25.05226042060194
3	21.1570331192545	22.463669563027327	29.678881752927843	26.70041556479033
4	16.8729379171389	16.18990051630777	32.522856063468076	34.41430550308525
5	16.222641984636695	35.24039793476892	32.70066742223901	15.836292658355372
6	35.49527767283717	33.31922931620703	15.154262687318978	16.031230323636823
7	29.3212441757965	29.535323007177936	20.710238005289007	20.43319481173656
8	26.785039667548165	32.93338370482307	20.070016370734166	20.211560256894597
9	26.395668051882637	14.58657599798514	19.6337992696134	39.38395668051883
10	17.6748520337489	26.87218234479285	32.70318599672585	22.7497796247324
11	36.30323636821559	21.20790832388868	20.995340637199345	21.49351467069639
12	21.85367082231457	25.070394156907188	27.653444150610756	25.422490870167486
13	30.89535323007178	19.491751668555597	23.93653192293162	25.676363178441004
14	23.439365319229317	20.898123662007304	23.36431179952147	32.29819921924191
15	26.293413927716912	27.450447046971416	21.365067371867525	24.89107165344415
16	26.32011081727742	25.739831255509383	22.80115854426395	25.138899382949248
17	24.725853167107417	26.140788313814383	24.07908323888679	25.054275280191412
18	24.225664274020904	24.95605087520463	25.59778365445158	25.220501196322882
19	25.97959954665659	25.055786424883514	25.131847374386098	23.832766654073794
20	25.6637703060068	24.438231960710237	25.386223397556982	24.51177433572598
21	25.614909960962095	25.40939428283592	24.053897494018386	24.921798262183604
22	25.922176048356633	24.50623347185493	24.180329933257777	25.391260546530663
23	25.038660118373002	25.16257398312555	24.269487470091928	25.52927842840952
24	26.293413927716912	25.10162448054401	23.977836544515803	24.627125047223274
25	25.966502959325023	24.342022415312933	24.337992696133988	25.353481929228057
26	25.665785165596272	24.950510011333584	24.179826218360407	25.203878604709733
27	25.652688578264705	24.240272006044577	24.349578138773452	25.757461276917265
28	24.775217227049488	24.63216219619695	25.10666162951769	25.485958947235865
29	25.03916383327037	25.09155018259665	24.85883390001259	25.010452084120388
30	24.84271502329681	25.858707971288254	24.55912353607858	24.739453469336357
31	25.470847500314818	24.67749653696008	24.89610880241783	24.955547160307265
32	24.62259161314696	24.57473869789699	25.050245561012467	25.752424127943584
33	24.814003274146835	24.478529152499682	24.974688326407254	25.73277924694623
34	25.40788313814381	24.302228938420853	24.739453469336357	25.55043445409898
35	25.35297821433069	24.164211056541998	25.066868152625617	25.4159425765017
36	24.83364815514419	24.407505351970784	25.32074046089913	25.438106031985896
37	25.64462913990681	22.762876212063972	25.602317088527894	25.99017755950132
38	24.144062460647273	23.560256894597657	26.970910464676994	25.324770180078076
39	24.147084750031482	22.89233094068757	27.479158796121396	25.48142551315955
40	23.938043067623724	22.545775091298324	28.02468202997104	25.491499811106916
41	23.014229945850648	23.079209167611133	28.409016496662886	25.497544389875333
42	23.776854300465935	22.370986021911598	28.006044578768417	25.84611509885405
43	22.394156907190528	22.78655081224027	28.565671829744367	26.253620450824833
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7.0
1	7.0
2	7.0
3	12.5
4	18.0
5	18.0
6	22.0
7	26.0
8	34.0
9	42.0
10	63.0
11	84.0
12	84.0
13	127.5
14	171.0
15	296.5
16	422.0
17	450.0
18	478.0
19	478.0
20	635.0
21	792.0
22	968.0
23	1144.0
24	1372.0
25	1600.0
26	1600.0
27	1820.0
28	2040.0
29	2924.5
30	3809.0
31	4142.5
32	4476.0
33	4476.0
34	5242.0
35	6008.0
36	6701.5
37	7395.0
38	8824.5
39	10254.0
40	10254.0
41	11276.0
42	12298.0
43	12100.0
44	11902.0
45	12819.5
46	13737.0
47	13737.0
48	14426.0
49	15115.0
50	15992.0
51	16869.0
52	18039.0
53	19209.0
54	19209.0
55	16731.0
56	14253.0
57	13550.5
58	12848.0
59	11567.0
60	10286.0
61	10286.0
62	9635.0
63	8984.0
64	8018.5
65	7053.0
66	6254.5
67	5456.0
68	5456.0
69	4762.0
70	4068.0
71	3562.0
72	3056.0
73	2474.0
74	1892.0
75	1892.0
76	1498.5
77	1105.0
78	917.0
79	729.0
80	567.5
81	406.0
82	406.0
83	318.5
84	231.0
85	182.5
86	134.0
87	105.5
88	77.0
89	77.0
90	56.5
91	36.0
92	21.5
93	7.0
94	4.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	198525.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.5753264086641
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.27880269970689	54.35796091200509
2	6.068215856071111	7.4730474411161945
3	1.731991171409967	3.1994376514947955
4	0.8889492507602036	2.1894936110518772
5	0.5281406163785405	1.6260215421590845
6	0.36225524172234164	1.338359085138162
7	0.2894997289566832	1.2478228214009286
8	0.1999431770151433	0.9849253110314202
9	0.1561699885381779	0.8654596217528072
>10	1.3020653906429716	15.859232123549303
>50	0.13826357001497772	5.8419388520288065
>100	0.055703308783012606	5.016301027271538
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	260	0.1309658733157033	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	257	0.12945472862359905	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	250	0.1259287243420224	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	236	0.11887671577886917	No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC	235	0.11837300088150106	No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG	216	0.10880241783150736	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	214	0.10779498803677118	No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC	212	0.10678755824203501	No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC	202	0.10175040926835412	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	5.037148973680896E-4	0.0
23	0.0	0.0	0.0	0.0010074297947361792	0.0
24	0.0	0.0	0.0	0.003022289384208538	0.0
25	0.0	0.0	0.0	0.0055408638710489864	0.0
26	0.0	0.0	0.0	0.008059438357889434	0.0
27	0.0	0.0	0.0	0.024682029971036395	0.0
28	0.0	0.0	0.0	0.07152751542626873	0.0
29	0.0	0.0	0.0	0.12643243923939051	0.0
30	0.0	0.0	0.0	0.20299710363934012	0.0
31	0.0	0.0	0.0	0.4624102757839063	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGAGT	20	0.0018382702	37.0	1
GGTATCA	115	0.0	28.956522	1
GCGGTTC	40	0.001925594	23.125002	11
CTTATAC	125	4.0563464E-10	19.24	37
GTATCAA	190	0.0	18.5	2
TAGGACT	50	0.0070148963	18.5	4
TAATGCA	50	0.0070148963	18.5	4
TCTTATA	200	0.0	16.650002	37
AGGACAC	135	2.1871529E-8	16.444443	5
AGTTCCC	95	7.012761E-5	15.578948	25
ACATCAA	95	7.012761E-5	15.578948	17
TAGGACA	95	7.012761E-5	15.578948	4
CAAAAGT	100	1.0861438E-4	14.8	21
AACTTGG	100	1.0861438E-4	14.8	28
TATCAAC	255	0.0	14.509804	3
TTAGGAC	115	2.1915841E-5	14.478261	3
CACATCA	115	2.1915841E-5	14.478261	16
GCTTAGG	115	2.1915841E-5	14.478261	1
CCATTGT	90	8.231579E-4	14.388889	16
ATCAACG	235	3.6379788E-12	14.170214	4
>>END_MODULE