FastQCFastQC Report
Fri 10 Feb 2017
ERR1632298.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632298.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1461019
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT54200.37097395721753107No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT42300.2895239555406192No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT37830.258928870877107No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19160.13114134723778403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12950.028.4285721
CGTCTAA555.144011E-420.1818181
TACGAAC500.00703577218.528
GTATCAA21950.016.6036452
GTCTAGA1504.6729838E-916.0333331
GTTCTAG1455.3480107E-815.3103451
TTAAGGT855.365387E-415.2352944
CGCATCG1101.4523248E-515.13636413
TCTATAC1601.0968506E-815.0312513
TATCCCG750.004105113414.85
AATAACG1405.9975355E-714.5357142
TAACGCC1152.2105452E-514.478264
TCGTATT1152.2105452E-514.4782626
CGGTGTA1051.6561162E-414.09523834
CTTATAC10550.014.02843737
TCTTATA17200.013.98255837
ACGCTAT800.006299698713.87500110
ATAACGA1907.1286195E-913.63157912
ATAGTCC1907.1286195E-913.6315793
CGTCAAT950.001245356413.63157919
Produced by FastQC (version 0.11.5)