##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632298.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1461019
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.549935353339006	31.0	31.0	34.0	30.0	34.0
2	31.73529913026456	31.0	31.0	34.0	30.0	34.0
3	31.82864904563185	31.0	31.0	34.0	30.0	34.0
4	35.61005777474489	37.0	35.0	37.0	33.0	37.0
5	34.88249639463963	37.0	35.0	37.0	32.0	37.0
6	34.846090297251436	36.0	35.0	37.0	32.0	37.0
7	34.83075579441472	35.0	35.0	37.0	32.0	37.0
8	34.77606451387696	35.0	35.0	37.0	32.0	37.0
9	36.39885928930425	38.0	35.0	39.0	32.0	39.0
10	36.12383138070073	37.0	35.0	39.0	32.0	39.0
11	36.301718184363104	38.0	35.0	39.0	32.0	39.0
12	36.21044969298825	37.0	35.0	39.0	32.0	39.0
13	36.31865636244292	38.0	35.0	39.0	32.0	39.0
14	37.33374309300564	39.0	36.0	40.0	32.0	41.0
15	37.2681477790501	39.0	36.0	40.0	32.0	41.0
16	37.253173983363666	39.0	36.0	40.0	32.0	41.0
17	37.22118398186471	39.0	36.0	40.0	32.0	41.0
18	37.219154576360744	39.0	36.0	40.0	32.0	41.0
19	37.25588989602463	39.0	36.0	40.0	32.0	41.0
20	37.212899353122715	39.0	36.0	40.0	32.0	41.0
21	37.18399897605712	39.0	36.0	40.0	32.0	41.0
22	37.12594360511397	39.0	36.0	40.0	32.0	41.0
23	37.05338260488057	39.0	36.0	40.0	31.0	41.0
24	36.9793534512556	39.0	36.0	40.0	31.0	41.0
25	36.88650044934392	38.0	36.0	40.0	31.0	41.0
26	36.73189055036245	38.0	36.0	40.0	31.0	41.0
27	36.604361750257866	38.0	35.0	40.0	30.0	41.0
28	36.50159375066306	38.0	35.0	40.0	30.0	41.0
29	36.38346455453351	38.0	35.0	40.0	30.0	41.0
30	36.26158523605785	38.0	35.0	40.0	30.0	41.0
31	36.13980721674393	38.0	35.0	40.0	30.0	41.0
32	36.001433246248	38.0	35.0	40.0	29.0	41.0
33	35.881659992101405	38.0	34.0	40.0	29.0	41.0
34	35.793774071384426	38.0	34.0	40.0	29.0	41.0
35	35.74658440444649	38.0	34.0	40.0	29.0	41.0
36	35.68381862248198	38.0	34.0	40.0	29.0	41.0
37	35.603446635533146	38.0	34.0	40.0	28.0	41.0
38	35.53408956351697	38.0	34.0	40.0	28.0	41.0
39	35.45607894216297	38.0	34.0	40.0	28.0	41.0
40	35.362968585624145	38.0	34.0	40.0	27.0	41.0
41	35.29616863298835	38.0	34.0	40.0	27.0	41.0
42	35.21618199352643	38.0	34.0	40.0	27.0	41.0
43	34.280848503681334	37.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	6.0
14	9.0
15	23.0
16	51.0
17	99.0
18	149.0
19	249.0
20	535.0
21	935.0
22	1624.0
23	2557.0
24	4116.0
25	6120.0
26	9209.0
27	13227.0
28	18446.0
29	25842.0
30	34895.0
31	46136.0
32	61457.0
33	83226.0
34	114627.0
35	158390.0
36	217291.0
37	277303.0
38	280042.0
39	104453.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.18891061649438	19.59173699999795	12.537687737120462	25.681664646387215
2	19.36449833985732	21.57206716681987	33.28272938271166	25.78070511061116
3	19.764698474147153	23.097235559564933	30.305629153351187	26.83243681293672
4	14.763120808148287	16.306701007995105	35.24683799457776	33.68334018927885
5	14.94498018164035	35.921367210145796	33.989838598950456	15.143814009263398
6	33.47410266396262	35.94169548787524	15.892469570895381	14.691732277266755
7	28.839666013925896	30.722050842596843	21.510124098317682	18.928159045159575
8	27.714355528572867	32.485340710832645	20.193234995575008	19.607068765019484
9	26.848932149410786	13.92042129500027	20.03197768133063	39.19866887425831
10	16.814291942815256	26.648387187298727	33.40983245255537	23.127488417330643
11	35.83957498157108	21.799853389996983	21.630382630205354	20.73018899822658
12	21.918127005877405	25.77652994245797	29.115980011211352	23.189363040453273
13	30.841419584550234	19.687765867521232	24.325556341156414	25.145258206772127
14	22.766644376288056	20.62635735743341	25.529031449967455	31.077966816311083
15	25.22403883864618	27.602926450648486	22.42523882304063	24.747795887664704
16	24.761827190474595	26.0641374273709	24.310087685375755	24.863947696778755
17	23.370674850908852	26.257974742286034	25.820403430756205	24.55094697604891
18	23.596544603458273	25.05627921334356	26.866522611957816	24.480653571240346
19	24.163546127736872	25.37585069051121	26.981100177341975	23.479503004409935
20	24.672779751666475	25.01007858214027	26.48637697387919	23.830764692314062
21	25.10467009669279	25.275509764075622	25.811231749895107	23.80858838933648
22	25.12492992904268	25.128078416502454	25.962016921066734	23.784974733388133
23	24.751765719679213	25.02965396069456	25.865166708988724	24.353413610637507
24	23.97710091381426	25.386391278963515	25.977211795329154	24.65929601189307
25	24.555259034961217	24.77106731671525	26.14599810132517	24.527675546998363
26	23.90653372748746	25.28974640302419	26.265982851694602	24.537737017793745
27	24.047394318622825	25.2373172422809	26.249761296738782	24.46552714235749
28	24.046983646345463	25.246488923141996	26.55324810970973	24.15327932080281
29	23.74616620317737	25.453878423210103	26.69999500348729	24.099960370125235
30	23.772175447410334	25.90575481906806	26.15393776535418	24.168131968167422
31	24.1932514224661	25.77680372397621	26.0428509143276	23.987093939230085
32	23.56629174569256	25.569071997010305	26.293429448898337	24.571206808398795
33	23.407019347455442	25.41411165768549	26.73531281934047	24.443556175518594
34	24.131855917000394	24.68824840744713	26.835859081914748	24.34403659363773
35	23.63911762954486	24.976198119257862	27.249269174459744	24.135415076737537
36	23.49750413923433	25.223901947887057	26.62990693481741	24.648686978061203
37	23.619063133333654	24.273264071172242	27.321547495275556	24.786125300218544
38	23.038509423902084	24.416725586730905	27.8057985556656	24.73896643370141
39	22.897511942007597	24.17538717840083	28.340014743134756	24.587086136456815
40	22.638993743407852	23.78107334675319	29.06717845558477	24.51275445425419
41	22.2038864655422	23.57710611566311	29.384970352883844	24.834037065910845
42	21.917237215943118	23.712217294915398	29.589485147010407	24.781060342131074
43	21.469193761340545	23.008941019931978	29.921103010980694	25.600762207746786
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	224.0
1	236.5
2	249.0
3	549.0
4	849.0
5	849.0
6	1114.5
7	1380.0
8	1354.0
9	1328.0
10	1892.5
11	2457.0
12	2457.0
13	4113.5
14	5770.0
15	8525.0
16	11280.0
17	10954.0
18	10628.0
19	10628.0
20	12122.5
21	13617.0
22	13441.5
23	13266.0
24	15923.0
25	18580.0
26	18580.0
27	21865.0
28	25150.0
29	29771.0
30	34392.0
31	39168.5
32	43945.0
33	43945.0
34	50113.5
35	56282.0
36	61846.0
37	67410.0
38	71235.5
39	75061.0
40	75061.0
41	79923.0
42	84785.0
43	88864.5
44	92944.0
45	95154.5
46	97365.0
47	97365.0
48	98720.0
49	100075.0
50	100559.5
51	101044.0
52	101106.5
53	101169.0
54	101169.0
55	99040.0
56	96911.0
57	92873.5
58	88836.0
59	83924.0
60	79012.0
61	79012.0
62	72827.0
63	66642.0
64	59860.0
65	53078.0
66	46421.0
67	39764.0
68	39764.0
69	33711.0
70	27658.0
71	23323.5
72	18989.0
73	15341.0
74	11693.0
75	11693.0
76	9340.0
77	6987.0
78	5889.0
79	4791.0
80	3970.5
81	3150.0
82	3150.0
83	2556.5
84	1963.0
85	1527.0
86	1091.0
87	891.0
88	691.0
89	691.0
90	522.0
91	353.0
92	236.5
93	120.0
94	75.0
95	30.0
96	30.0
97	19.0
98	8.0
99	5.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1461019.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.68891416477955
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.5036889319568	65.78614876370918
2	5.820438819670861	8.461627555288121
3	1.4034478118190665	3.0604529258419144
4	0.6063384390699781	1.7629633100945619
5	0.3449054411606718	1.2535401003746747
6	0.2196934474016727	0.9581566884446822
7	0.1492922751042836	0.7596325359363948
8	0.12102605826328995	0.7037802208641514
9	0.09241067467286652	0.6045508439284826
>10	0.6455612770534678	9.112972317370977
>50	0.06799879874725923	3.3845110434367576
>100	0.02453989638732293	2.9870656866396557
>500	2.8205515390541247E-4	0.11563287539787816
>1k	2.8205515390541247E-4	0.6785572221191088
>5k	9.40183846351375E-5	0.3704079105534868
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5420	0.37097395721753107	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4230	0.2895239555406192	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3783	0.258928870877107	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1916	0.13114134723778403	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	6.844537956043008E-5	0.0
4	0.0	0.0	0.0	6.844537956043008E-5	0.0
5	0.0	0.0	0.0	6.844537956043008E-5	0.0
6	0.0	0.0	0.0	6.844537956043008E-5	0.0
7	0.0	0.0	0.0	6.844537956043008E-5	0.0
8	0.0	0.0	0.0	6.844537956043008E-5	0.0
9	0.0	0.0	0.0	6.844537956043008E-5	0.0
10	0.0	0.0	0.0	6.844537956043008E-5	0.0
11	0.0	0.0	0.0	2.0533613868129025E-4	0.0
12	0.0	0.0	0.0	3.4222689780215044E-4	0.0
13	0.0	0.0	0.0	3.4222689780215044E-4	0.0
14	0.0	0.0	0.0	4.791176569230106E-4	0.0
15	0.0	0.0	0.0	6.160084160438707E-4	0.0
16	0.0	0.0	0.0	7.528991751647309E-4	0.0
17	0.0	0.0	0.0	0.0010266806934064513	0.0
18	0.0	0.0	0.0	0.0010266806934064513	0.0
19	0.0	0.0	0.0	0.0011635714525273114	0.0
20	0.0	0.0	0.0	0.001574243729889892	0.0
21	0.0	0.0	0.0	0.0020533613868129025	0.0
22	0.0	0.0	0.0	0.003148487459779784	0.0
23	0.0	0.0	0.0	0.004722731189669676	0.0
24	0.0	0.0	0.0	0.006228529539999137	0.0
25	0.0	0.0	0.0	0.00800810940857032	0.0
26	0.0	0.0	0.0	0.010951260729668814	0.0
27	0.0	0.0	0.0	0.028062605619776335	0.0
28	0.0	6.844537956043008E-5	0.0	0.08767853121691094	0.0
29	0.0	6.844537956043008E-5	0.0	0.17830021375492036	0.0
30	0.0	6.844537956043008E-5	0.0	0.30909933409490226	0.0
31	0.0	6.844537956043008E-5	0.0	0.6288761474012317	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1295	0.0	28.428572	1
CGTCTAA	55	5.144011E-4	20.181818	1
TACGAAC	50	0.007035772	18.5	28
GTATCAA	2195	0.0	16.603645	2
GTCTAGA	150	4.6729838E-9	16.033333	1
GTTCTAG	145	5.3480107E-8	15.310345	1
TTAAGGT	85	5.365387E-4	15.235294	4
CGCATCG	110	1.4523248E-5	15.136364	13
TCTATAC	160	1.0968506E-8	15.031251	3
TATCCCG	75	0.0041051134	14.8	5
AATAACG	140	5.9975355E-7	14.535714	2
TAACGCC	115	2.2105452E-5	14.47826	4
TCGTATT	115	2.2105452E-5	14.47826	26
CGGTGTA	105	1.6561162E-4	14.095238	34
CTTATAC	1055	0.0	14.028437	37
TCTTATA	1720	0.0	13.982558	37
ACGCTAT	80	0.0062996987	13.875001	10
ATAACGA	190	7.1286195E-9	13.631579	12
ATAGTCC	190	7.1286195E-9	13.631579	3
CGTCAAT	95	0.0012453564	13.631579	19
>>END_MODULE