FastQCFastQC Report
Fri 10 Feb 2017
ERR1632297.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632297.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28082
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT6222.214941955701161No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGT6122.179331956413361No Hit
CATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC3831.3638629727227407No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCC3681.3104479737910406No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATG3201.1395199772096005No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2050.7300049853999003No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1540.5483939890321202No Hit
CTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTC1330.4736129905277402Illumina PCR Primer Index 7 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1250.4451249910975002No Hit
CACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCG1110.39527099209458016No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT570.20297699594046006No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTG360.12819599743608004No Hit
TTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTA350.12463499750730006No Hit
GTTCCGACCTGCACGAATGGCGTAACGATGGCCACACTGTCTC300.10682999786340004No Hit
TATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTAT290.10326899793462004No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC950.031.1578963
TTATACA950.031.1578962
CCGTCTT850.030.47058937
GCCGTCT800.030.062536
TACACAT1050.028.1904755
ATACACA1100.026.909094
TGCCGTC959.094947E-1225.3157935
ATGCCGT959.094947E-1225.3157934
CTTATAC1200.024.6666681
TATGCCG1001.8189894E-1124.0533
CACATCT1300.022.769237
ACACATC1300.022.769236
ACATCTC1350.021.9259268
CAGTCGG450.003734912720.55555710
GCAGTCG450.003734912720.5555579
GGCAGTC450.003734912720.5555578
GTGATTC554.973305E-420.18181816
ATCTCCG1500.019.73333410
GTATGCC1253.5652192E-1019.2432
CATCTCC1601.8189894E-1218.59
Produced by FastQC (version 0.11.5)