##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632297.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	28082
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.535538779289226	34.0	31.0	34.0	31.0	34.0
2	32.694252546114946	34.0	31.0	34.0	31.0	34.0
3	32.718752225624954	34.0	31.0	34.0	31.0	34.0
4	36.232319635353605	37.0	37.0	37.0	35.0	37.0
5	36.11416565771669	37.0	35.0	37.0	35.0	37.0
6	36.13955558720889	37.0	35.0	37.0	35.0	37.0
7	36.1205398475892	37.0	35.0	37.0	35.0	37.0
8	36.10280606794388	37.0	35.0	37.0	35.0	37.0
9	37.85310875293783	39.0	38.0	39.0	35.0	39.0
10	37.83263300334734	39.0	37.0	39.0	35.0	39.0
11	37.88700947225981	39.0	38.0	39.0	35.0	39.0
12	37.838223773235526	39.0	38.0	39.0	35.0	39.0
13	37.91998433160031	39.0	38.0	39.0	35.0	39.0
14	39.14397122712057	40.0	38.0	41.0	36.0	41.0
15	39.14977565700449	40.0	38.0	41.0	36.0	41.0
16	39.09176696816466	40.0	38.0	41.0	36.0	41.0
17	39.067267288654655	40.0	38.0	41.0	36.0	41.0
18	39.16245281675094	40.0	39.0	41.0	36.0	41.0
19	39.19332668613347	40.0	39.0	41.0	36.0	41.0
20	39.202727725945444	40.0	39.0	41.0	36.0	41.0
21	39.18047147639057	40.0	39.0	41.0	36.0	41.0
22	39.033224129335515	40.0	38.0	41.0	36.0	41.0
23	39.10038458799231	40.0	39.0	41.0	36.0	41.0
24	38.95701873085962	40.0	38.0	41.0	35.0	41.0
25	38.84007549319849	40.0	38.0	41.0	35.0	41.0
26	38.771704294565914	40.0	38.0	41.0	35.0	41.0
27	38.587315718253684	40.0	38.0	41.0	35.0	41.0
28	38.64208389715832	40.0	38.0	41.0	35.0	41.0
29	38.49398191012036	40.0	38.0	41.0	35.0	41.0
30	38.400683711986325	40.0	38.0	41.0	34.0	41.0
31	38.2845951143081	40.0	38.0	41.0	34.0	41.0
32	38.0650950786981	40.0	37.0	41.0	34.0	41.0
33	37.99131116017378	40.0	37.0	41.0	33.0	41.0
34	37.88166797236664	40.0	37.0	41.0	33.0	41.0
35	37.81742753365145	40.0	37.0	41.0	33.0	41.0
36	37.78178192436436	40.0	37.0	41.0	33.0	41.0
37	37.68285734634285	40.0	37.0	41.0	33.0	41.0
38	37.62189302756214	40.0	37.0	41.0	33.0	41.0
39	37.47172566056549	40.0	36.0	41.0	33.0	41.0
40	37.351684352966316	39.0	36.0	41.0	33.0	41.0
41	37.22402250551955	39.0	36.0	41.0	32.0	41.0
42	37.06050138878997	39.0	35.0	41.0	32.0	41.0
43	36.07844882843102	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	1.0
19	2.0
20	1.0
21	4.0
22	6.0
23	11.0
24	30.0
25	38.0
26	57.0
27	103.0
28	137.0
29	211.0
30	256.0
31	367.0
32	409.0
33	645.0
34	889.0
35	1298.0
36	2155.0
37	4083.0
38	8320.0
39	9058.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.146713197065736	17.769389644612207	15.533081689338365	26.55081546898369
2	19.026422619471546	20.009258599814828	33.93276832134464	27.03155045936899
3	18.848372623032546	22.21707855565843	34.901360301972794	24.03318851933623
4	13.449896731002065	18.378320632433585	29.36044441279111	38.811338223773234
5	14.503952709920945	34.29955131400897	33.18139733637205	18.015098639698028
6	31.589630368207395	32.78968734420625	16.341428673171425	19.27925361441493
7	25.45402749091945	31.607435367851295	18.132611637347768	24.80592550388149
8	31.429385371412295	29.85898440282031	18.88754362224913	19.824086603518268
9	25.37924649241507	12.541841749163165	16.526600669467985	45.55231108895378
10	20.69297058614059	24.87002350259953	25.73890748522185	28.698098426038033
11	34.976141300477174	20.26565059468699	18.253685634926285	26.504522469909553
12	20.46862759062745	28.78356242432875	23.224841535503167	27.52296844954063
13	35.207606295847874	15.921230681575386	22.61591054768179	26.255252474894952
14	24.10440851791183	21.46570757068585	22.006979559860408	32.422904351541916
15	29.880350402392992	23.79816252403675	18.837689623246206	27.483797450324047
16	29.43522541129549	21.889466562210668	21.46570757068585	27.20960045580799
17	21.063314578733706	31.450751370984975	21.23780357524393	26.24813047503739
18	25.82437148351257	19.770671604586568	24.35367851292643	30.051278398974436
19	24.36436151271277	25.68193148636137	25.268855494622887	24.68485150630297
20	25.518125489637487	20.83541058329179	24.43914251121715	29.20732141585357
21	31.888754362224915	19.642475607150487	23.26401253471975	25.20475749590485
22	26.67545046649099	26.47603447047931	21.319706573605867	25.52880848942383
23	27.48735845025283	20.23716259525675	22.59810554803789	29.677373406452535
24	27.747311445053768	23.61299052774019	24.78099850438003	23.85869952282601
25	23.54889252902215	23.03610853927783	24.46050851078983	28.95449042091019
26	28.83697742326045	21.362438572751227	23.63791752724165	26.162666476746672
27	23.53820952923581	25.870664482586708	24.232604515347912	26.358521472829572
28	24.143579517128412	20.77487358450253	25.47183249056335	29.60971440780571
29	24.74538850509223	23.43137953137241	26.44398547112029	25.37924649241507
30	23.05035253899295	21.08468057830639	29.06488141870237	26.80008546399829
31	26.753792464924153	22.05327255893455	22.85805854283883	28.33487643330247
32	24.22904351541913	25.31158749376825	27.341357453172847	23.118011537639767
33	22.22063955558721	21.29834057403319	28.33487643330247	28.146143437077132
34	26.15910547681789	24.49611851007763	24.65636350687273	24.68841250623175
35	22.43429955131401	21.230681575386367	32.34456235310875	23.990456520190868
36	24.15426251691475	22.78683854426323	23.972651520546968	29.08624741827505
37	22.33103055337939	23.21059753578805	27.14906345701873	27.30930845381383
38	22.886546542269066	20.52204258955915	28.67673242646535	27.91467844170643
39	24.004700519905988	20.796239584075206	27.768677444626448	27.430382451392347
40	21.009899579802006	19.015739619685206	33.97906132041877	25.99529948009401
41	22.23488355530233	19.970087600598248	29.09693041806139	28.698098426038033
42	20.94224058115519	19.567694608646107	33.29891033402179	26.19115447617691
43	19.653158606936827	19.428815611423687	33.15647033687059	27.76155544476889
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	1.0
2	2.0
3	8.5
4	15.0
5	15.0
6	26.0
7	37.0
8	32.0
9	27.0
10	38.5
11	50.0
12	50.0
13	97.5
14	145.0
15	235.5
16	326.0
17	289.0
18	252.0
19	252.0
20	261.5
21	271.0
22	193.5
23	116.0
24	118.5
25	121.0
26	121.0
27	155.0
28	189.0
29	200.5
30	212.0
31	256.5
32	301.0
33	301.0
34	312.5
35	324.0
36	369.5
37	415.0
38	539.0
39	663.0
40	663.0
41	775.5
42	888.0
43	1131.0
44	1374.0
45	1649.0
46	1924.0
47	1924.0
48	2251.0
49	2578.0
50	2519.5
51	2461.0
52	2881.5
53	3302.0
54	3302.0
55	2676.5
56	2051.0
57	2565.5
58	3080.0
59	2663.5
60	2247.0
61	2247.0
62	1782.5
63	1318.0
64	1139.0
65	960.0
66	910.5
67	861.0
68	861.0
69	742.5
70	624.0
71	522.0
72	420.0
73	347.5
74	275.0
75	275.0
76	201.5
77	128.0
78	97.5
79	67.0
80	51.5
81	36.0
82	36.0
83	24.5
84	13.0
85	8.0
86	3.0
87	3.5
88	4.0
89	4.0
90	3.0
91	2.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	28082.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.05035253899294
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.53397679633422	65.40488569190228
2	6.722238471287902	9.821237803575244
3	1.73052549478405	3.7924649241507016
4	0.7214585161353222	2.1081119578377607
5	0.33148093984595883	1.2107399757852004
6	0.19498878814468168	0.8546399829072003
7	0.2096129472555328	1.0718609785627804
8	0.12186799259042605	0.7121999857560003
9	0.053621916739787466	0.35253899294922014
>10	0.3266062201423418	3.6678299266434014
>50	0.004874719703617042	0.20297699594046006
>100	0.038997757628936335	6.406238871875222
>500	0.009749439407234084	4.394273912114522
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT	622	2.214941955701161	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGT	612	2.179331956413361	No Hit
CATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC	383	1.3638629727227407	No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCC	368	1.3104479737910406	No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATG	320	1.1395199772096005	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	205	0.7300049853999003	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	154	0.5483939890321202	No Hit
CTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTC	133	0.4736129905277402	Illumina PCR Primer Index 7 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	125	0.4451249910975002	No Hit
CACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCG	111	0.39527099209458016	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	57	0.20297699594046006	No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTG	36	0.12819599743608004	No Hit
TTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTA	35	0.12463499750730006	No Hit
GTTCCGACCTGCACGAATGGCGTAACGATGGCCACACTGTCTC	30	0.10682999786340004	No Hit
TATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTAT	29	0.10326899793462004	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0035609999287800013	0.0
10	0.0	0.0	0.0	0.0035609999287800013	0.0
11	0.0	0.0	0.0	0.0035609999287800013	0.0
12	0.0	0.0	0.0	0.0071219998575600026	0.0
13	0.0	0.0	0.0	0.0071219998575600026	0.0
14	0.0	0.0	0.0	0.0071219998575600026	0.0
15	0.0	0.0	0.0	0.010682999786340004	0.0
16	0.0	0.0	0.0	0.010682999786340004	0.0
17	0.0	0.0	0.0	0.010682999786340004	0.0
18	0.0	0.0	0.0	0.010682999786340004	0.0
19	0.0	0.0	0.0	0.010682999786340004	0.0
20	0.0	0.0	0.0	0.014243999715120005	0.0035609999287800013
21	0.0	0.0	0.0	0.017804999643900008	0.0035609999287800013
22	0.0	0.0	0.0	0.021365999572680008	0.0035609999287800013
23	0.0	0.0	0.0	0.03204899935902001	0.0035609999287800013
24	0.0	0.0	0.0	0.042731999145360015	0.0035609999287800013
25	0.0	0.0	0.0	0.042731999145360015	0.0035609999287800013
26	0.0	0.0	0.0	0.042731999145360015	0.0035609999287800013
27	0.0	0.0	0.0	0.07478099850438003	0.0035609999287800013
28	0.0	0.0	0.0	0.12463499750730005	0.0035609999287800013
29	0.0	0.0	0.0	0.2991239940175201	0.0035609999287800013
30	0.0	0.0	0.0	0.5056619898867603	0.0035609999287800013
31	0.0	0.0	0.0	1.1074709778505805	0.0035609999287800013
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACAC	95	0.0	31.157896	3
TTATACA	95	0.0	31.157896	2
CCGTCTT	85	0.0	30.470589	37
GCCGTCT	80	0.0	30.0625	36
TACACAT	105	0.0	28.190475	5
ATACACA	110	0.0	26.90909	4
TGCCGTC	95	9.094947E-12	25.31579	35
ATGCCGT	95	9.094947E-12	25.31579	34
CTTATAC	120	0.0	24.666668	1
TATGCCG	100	1.8189894E-11	24.05	33
CACATCT	130	0.0	22.76923	7
ACACATC	130	0.0	22.76923	6
ACATCTC	135	0.0	21.925926	8
CAGTCGG	45	0.0037349127	20.555557	10
GCAGTCG	45	0.0037349127	20.555557	9
GGCAGTC	45	0.0037349127	20.555557	8
GTGATTC	55	4.973305E-4	20.181818	16
ATCTCCG	150	0.0	19.733334	10
GTATGCC	125	3.5652192E-10	19.24	32
CATCTCC	160	1.8189894E-12	18.5	9
>>END_MODULE