FastQCFastQC Report
Fri 10 Feb 2017
ERR1632296.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632296.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1186090
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT21450.1808463101450986No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17310.14594170762758307No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT14300.12056420676339907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8400.027.0892851
CCGTTTA551.9027251E-523.54545427
TCGTACC652.6829657E-622.769232
GTCTATA450.003825878720.5555551
GTCTACG450.003825878720.5555551
GTCGTAC851.2453766E-619.5882361
CTTATAC14700.018.537
TTAACGG500.00703501118.535
GGACCGT902.1523338E-618.56
GTCCTAT1351.1514203E-917.8148161
TAGACAA752.0673363E-417.2666665
TCCTATA752.0673363E-417.2666662
GTATCAA13450.017.0557612
TCTTATA24400.016.90778737
GTATTAG2450.016.6122441
TACCCCG1901.8189894E-1216.5526315
TCTAATA1504.669346E-916.0333332
AAAGTGT1851.8189894E-1116.06
GTGTAAT700.00259255915.8571431
ATCTTAC1059.343519E-615.8571421
Produced by FastQC (version 0.11.5)