##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632295.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3374864
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.30085034537688	33.0	31.0	34.0	31.0	34.0
2	32.48823952609646	34.0	31.0	34.0	31.0	34.0
3	32.58531988251971	34.0	31.0	34.0	31.0	34.0
4	36.13000494242139	37.0	35.0	37.0	35.0	37.0
5	35.87737994775493	37.0	35.0	37.0	35.0	37.0
6	35.878526364321644	37.0	35.0	37.0	35.0	37.0
7	35.853704030740204	37.0	35.0	37.0	35.0	37.0
8	35.825708828563165	37.0	35.0	37.0	35.0	37.0
9	37.55600373822471	39.0	37.0	39.0	35.0	39.0
10	37.445263275794225	39.0	37.0	39.0	35.0	39.0
11	37.54242363544131	39.0	37.0	39.0	35.0	39.0
12	37.4793882064581	39.0	37.0	39.0	35.0	39.0
13	37.52279084431254	39.0	37.0	39.0	35.0	39.0
14	38.804914805455866	40.0	38.0	41.0	36.0	41.0
15	38.786704293861916	40.0	38.0	41.0	36.0	41.0
16	38.78064656827653	40.0	38.0	41.0	35.0	41.0
17	38.7508412190832	40.0	38.0	41.0	35.0	41.0
18	38.73869317400642	40.0	38.0	41.0	35.0	41.0
19	38.759588830838815	40.0	38.0	41.0	35.0	41.0
20	38.729385835992204	40.0	38.0	41.0	35.0	41.0
21	38.69757003541476	40.0	38.0	41.0	35.0	41.0
22	38.657441603572764	40.0	38.0	41.0	35.0	41.0
23	38.60459917792243	40.0	38.0	41.0	35.0	41.0
24	38.54964111146405	40.0	38.0	41.0	34.0	41.0
25	38.48106294061035	40.0	38.0	41.0	34.0	41.0
26	38.37003268872464	40.0	38.0	41.0	34.0	41.0
27	38.28657777024496	40.0	38.0	41.0	34.0	41.0
28	38.21232381512262	40.0	38.0	41.0	34.0	41.0
29	38.13309306686136	40.0	38.0	41.0	34.0	41.0
30	38.05307828700653	40.0	38.0	41.0	33.0	41.0
31	37.97020265112905	40.0	37.0	41.0	33.0	41.0
32	37.8783592464763	40.0	37.0	41.0	33.0	41.0
33	37.7914464108776	40.0	37.0	41.0	33.0	41.0
34	37.71475265373657	40.0	37.0	41.0	33.0	41.0
35	37.691595868752046	40.0	37.0	41.0	33.0	41.0
36	37.65592361647758	40.0	37.0	41.0	33.0	41.0
37	37.59783772027554	40.0	37.0	41.0	33.0	41.0
38	37.55143822091794	39.0	37.0	41.0	33.0	41.0
39	37.50722962466043	39.0	37.0	41.0	33.0	41.0
40	37.43374073740453	39.0	36.0	41.0	32.0	41.0
41	37.38291172622067	39.0	36.0	41.0	32.0	41.0
42	37.329572391657855	39.0	36.0	41.0	32.0	41.0
43	36.40490520506901	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	1.0
12	1.0
13	0.0
14	3.0
15	10.0
16	20.0
17	32.0
18	101.0
19	172.0
20	370.0
21	683.0
22	1315.0
23	2257.0
24	3653.0
25	5773.0
26	8899.0
27	13036.0
28	18397.0
29	26220.0
30	35753.0
31	48541.0
32	65298.0
33	89003.0
34	127140.0
35	186625.0
36	295930.0
37	527794.0
38	1055585.0
39	862249.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.17424346581077	17.47261519278999	11.918643240142417	27.43449810125682
2	19.391003607849086	19.643310071161384	32.99303320074527	27.97265312024425
3	20.14229314129399	21.041025653181876	28.81621303851059	30.000468167013544
4	15.516595631705455	15.058591990669845	32.90227398792959	36.52253838969511
5	16.72879262690289	34.19868178391781	32.791869539039205	16.280656050140095
6	36.83250643581489	33.2173977973631	15.044428456968934	14.90566730985308
7	31.71040373775062	28.782552422853186	19.956922708589143	19.55012113080705
8	29.08025330798515	31.89752238905034	18.99620251364203	20.026021789322474
9	27.88784970298062	13.669024885150927	17.948486220481776	40.494639191386675
10	17.8234441447122	25.348754794267265	31.63688966429462	25.190911396725912
11	38.77951822651224	19.997339152036943	20.197406473268256	21.025736148182563
12	23.82125620469447	23.40429125440314	27.61100891769268	25.16344362320971
13	32.34613305899142	17.967183270199925	22.648705251530135	27.037978419278524
14	24.234576563677827	18.374251525394804	23.183363833327803	34.20780807759957
15	26.832696073086204	25.429350634573716	20.53718312797197	27.200770164368105
16	27.15552389666665	24.06588828468347	22.13330670509982	26.645281113550055
17	26.005314584528445	24.239524911226052	23.526370247808504	26.228790256437
18	26.38491506620711	23.376379018532305	24.04790237473273	26.190803540527856
19	26.865764072270764	23.535200233253846	23.714674132053915	25.884361562421475
20	27.050808565915546	23.30864295568651	23.365326721313807	26.27522175708414
21	27.49621910690327	23.427788497551308	22.970762673695887	26.105229721849533
22	27.69302703753396	23.218061527812676	22.870195658254673	26.218715776398692
23	27.019755462738647	23.096278842643734	23.16182222454001	26.722143470077608
24	26.720632298071862	23.350926141023756	23.036306055592163	26.892135505312215
25	26.92271451531084	22.677210103873815	23.380823642078614	27.01925173873673
26	26.522816919437346	23.24102541613529	23.50808802962134	26.728069634806022
27	26.633013952562234	23.153199654860167	23.327932621877505	26.88585377070009
28	26.519883467896783	23.291931171152378	23.49027990461245	26.69790545633839
29	26.28594811524257	23.56109757311702	23.624122335003722	26.528831976636692
30	26.2111895471936	23.76140194093747	23.371460301807716	26.655948210061204
31	26.63005087019803	23.431196042270148	23.1508291889688	26.787923898563022
32	25.993225208482475	23.481864750698104	23.50708058161751	27.017829459201913
33	25.89876214271153	23.161733332069083	24.131787236463452	26.807717288755935
34	26.54471409810884	22.559072010012848	24.11620142322772	26.78001246865059
35	26.0499978665807	22.81940842653215	24.684313204917295	26.44628050196986
36	25.83475956364464	22.733093837262775	24.493994424664223	26.938152174428364
37	25.571548957232054	21.985152586889427	25.072891826159516	27.370406629719007
38	24.992799709854975	21.97919679133737	25.93126715624689	27.096736342560767
39	25.003644591307978	21.50907414343215	26.375462833465292	27.11181843179459
40	24.768612898179008	21.492332728074377	27.07080937187395	26.66824500187267
41	24.00357466256418	21.312118058683254	27.77015607147429	26.91415120727828
42	23.650967861223446	21.393839870288105	28.05867140127721	26.896520867211244
43	23.013638475505978	20.740332054862062	28.55048381208843	27.695545657543537
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	35.0
1	37.5
2	40.0
3	130.0
4	220.0
5	220.0
6	330.0
7	440.0
8	434.0
9	428.0
10	615.0
11	802.0
12	802.0
13	1353.5
14	1905.0
15	3201.5
16	4498.0
17	4757.0
18	5016.0
19	5016.0
20	6350.5
21	7685.0
22	8709.5
23	9734.0
24	12930.5
25	16127.0
26	16127.0
27	20330.5
28	24534.0
29	31113.5
30	37693.0
31	46043.5
32	54394.0
33	54394.0
34	66317.0
35	78240.0
36	91994.0
37	105748.0
38	120929.5
39	136111.0
40	136111.0
41	152070.5
42	168030.0
43	181019.0
44	194008.0
45	207805.0
46	221602.0
47	221602.0
48	232237.0
49	242872.0
50	252905.0
51	262938.0
52	268697.5
53	274457.0
54	274457.0
55	273104.5
56	271752.0
57	265293.5
58	258835.0
59	250094.5
60	241354.0
61	241354.0
62	223491.0
63	205628.0
64	184890.0
65	164152.0
66	145062.5
67	125973.0
68	125973.0
69	108427.5
70	90882.0
71	77461.0
72	64040.0
73	52235.5
74	40431.0
75	40431.0
76	33109.5
77	25788.0
78	21137.0
79	16486.0
80	13500.5
81	10515.0
82	10515.0
83	8078.5
84	5642.0
85	4477.0
86	3312.0
87	2450.5
88	1589.0
89	1589.0
90	1172.5
91	756.0
92	441.0
93	126.0
94	78.0
95	30.0
96	30.0
97	21.0
98	12.0
99	8.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3374864.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.24994404738921
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.1890733596751	50.95326088634257
2	9.456805748256992	11.584576458955386
3	2.879803485146711	5.2916340699813755
4	1.3061724319587067	3.2001195349485227
5	0.7533066391208296	2.3069994748338813
6	0.48623734213668124	1.7869205999773783
7	0.33471234857735693	1.435077883563334
8	0.24082926683319175	1.1800623294805408
9	0.17662008778187285	0.9736173444856213
>10	1.0417190443041364	11.87338226735001
>50	0.08921801882678196	3.744222018681082
>100	0.043471332001409305	4.736178435055434
>500	0.0017891221220477061	0.7152256958469217
>1k	2.41773258319168E-4	0.21872300049789337
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.9630823642078615E-5	2.9630823642078615E-5
2	0.0	2.9630823642078615E-5	0.0	5.926164728415723E-5	2.9630823642078615E-5
3	0.0	2.9630823642078615E-5	0.0	5.926164728415723E-5	2.9630823642078615E-5
4	0.0	2.9630823642078615E-5	0.0	5.926164728415723E-5	2.9630823642078615E-5
5	0.0	2.9630823642078615E-5	0.0	5.926164728415723E-5	2.9630823642078615E-5
6	0.0	2.9630823642078615E-5	0.0	5.926164728415723E-5	2.9630823642078615E-5
7	0.0	2.9630823642078615E-5	0.0	5.926164728415723E-5	2.9630823642078615E-5
8	0.0	2.9630823642078615E-5	0.0	5.926164728415723E-5	8.889247092623585E-5
9	0.0	5.926164728415723E-5	0.0	1.1852329456831446E-4	8.889247092623585E-5
10	0.0	5.926164728415723E-5	0.0	1.1852329456831446E-4	8.889247092623585E-5
11	0.0	5.926164728415723E-5	0.0	1.777849418524717E-4	8.889247092623585E-5
12	0.0	5.926164728415723E-5	0.0	2.074157654945503E-4	8.889247092623585E-5
13	0.0	5.926164728415723E-5	0.0	2.074157654945503E-4	8.889247092623585E-5
14	0.0	5.926164728415723E-5	0.0	2.074157654945503E-4	8.889247092623585E-5
15	0.0	5.926164728415723E-5	0.0	2.3704658913662892E-4	8.889247092623585E-5
16	0.0	1.1852329456831446E-4	0.0	4.148315309891006E-4	8.889247092623585E-5
17	0.0	1.1852329456831446E-4	0.0	5.629856491994936E-4	8.889247092623585E-5
18	0.0	1.1852329456831446E-4	0.0	6.815089437678081E-4	1.1852329456831446E-4
19	0.0	1.1852329456831446E-4	0.0	7.70401414694044E-4	1.1852329456831446E-4
20	0.0	1.1852329456831446E-4	0.0	0.001007448003830673	1.4815411821039306E-4
21	0.0	1.1852329456831446E-4	0.0	0.0011852329456831445	2.3704658913662892E-4
22	0.0	1.1852329456831446E-4	0.0	0.001600064476672245	2.3704658913662892E-4
23	0.0	1.777849418524717E-4	0.0	0.0022815734204400533	2.3704658913662892E-4
24	0.0	2.3704658913662892E-4	0.0	0.004000161191680613	2.3704658913662892E-4
25	0.0	2.3704658913662892E-4	0.0	0.004800193430016735	2.3704658913662892E-4
26	0.0	2.3704658913662892E-4	0.0	0.006607673672183531	2.3704658913662892E-4
27	0.0	2.3704658913662892E-4	0.0	0.02311204244082132	2.3704658913662892E-4
28	0.0	3.2593906006286475E-4	0.0	0.09416675753452583	2.3704658913662892E-4
29	0.0	3.555698837049434E-4	0.0	0.2022896330044707	2.3704658913662892E-4
30	0.0	3.555698837049434E-4	0.0	0.35248827804616717	2.3704658913662892E-4
31	0.0	3.555698837049434E-4	0.0	0.8343743629372917	2.3704658913662892E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1130	0.0	26.19469	1
CTTATAC	3465	0.0	18.793652	37
GACGTAT	50	0.0070376354	18.5	1
GTATCAA	1920	0.0	15.223959	2
ACCGTCG	210	9.094947E-12	14.97619	8
TATACAC	1100	0.0	14.968182	37
CGTTATT	355	0.0	14.59155	2
TACCGTC	245	0.0	14.34694	7
TCTTATA	5575	0.0	14.235874	37
GACGGAC	470	0.0	14.170214	7
GTATAAA	315	0.0	14.095238	1
GCGTTAT	450	0.0	13.566667	1
ATCAACG	2955	0.0	13.522841	2
TCAACGC	3010	0.0	13.460134	3
ATAACGA	220	2.8558134E-10	13.454545	12
ATACCGT	265	1.8189894E-12	13.264152	6
CAACGCA	3075	0.0	13.17561	4
CGTCGTA	225	4.0745363E-10	13.155556	10
CGTATTG	380	0.0	12.657895	27
AGACGGA	570	0.0	12.657895	6
>>END_MODULE