FastQCFastQC Report
Fri 10 Feb 2017
ERR1632294.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632294.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14067
Sequences flagged as poor quality0
Sequence length43
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT700.49761853984502735TruSeq Adapter, Index 8 (95% over 22bp)
CATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTC190.13506788938650743No Hit
CTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTC160.11374138053600626RNA PCR Primer, Index 8 (95% over 23bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTAT400.001838387223.12500237
Produced by FastQC (version 0.11.5)