FastQCFastQC Report
Fri 10 Feb 2017
ERR1632293.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632293.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352779
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1450.026.7931021
TAGAGTG358.8571635E-426.428575
GCGGGTA358.8571635E-426.4285723
TATGCCG551.8978157E-523.54545433
ATATGAC400.001928549423.1253
AGAGTGT502.69696E-422.26
TATCAGT450.003820632620.5555556
CCCTAGA450.003820632620.5555551
ATATGTG450.003820632620.5555555
CCTAAGC450.003820632620.5555553
GAGCCGT450.003820632620.5555556
GCCGTCT656.8848116E-519.92307736
AGGTCGC851.240307E-619.58823610
TATATCA701.21616E-418.52
AGAGGTC1205.136826E-918.58
GTATCAA2200.017.659092
TCGCCAT1054.7740104E-717.61904713
CCGTCTT852.7144613E-517.41176437
TCTTATA5650.017.02654837
CTTATAC3400.016.86764737
Produced by FastQC (version 0.11.5)