##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632293.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	352779
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.54259749021342	34.0	31.0	34.0	31.0	34.0
2	32.725499533702404	34.0	31.0	34.0	31.0	34.0
3	32.80978743065772	34.0	31.0	34.0	31.0	34.0
4	36.27490014995224	37.0	37.0	37.0	35.0	37.0
5	36.110474262923816	37.0	35.0	37.0	35.0	37.0
6	36.12987451067099	37.0	35.0	37.0	35.0	37.0
7	36.10734765958291	37.0	35.0	37.0	35.0	37.0
8	36.086952454652916	37.0	35.0	37.0	35.0	37.0
9	37.840449686631004	39.0	38.0	39.0	35.0	39.0
10	37.77592203617562	39.0	37.0	39.0	35.0	39.0
11	37.86568644959026	39.0	38.0	39.0	35.0	39.0
12	37.81608882614895	39.0	38.0	39.0	35.0	39.0
13	37.84562289705453	39.0	38.0	39.0	35.0	39.0
14	39.19514200108283	40.0	39.0	41.0	36.0	41.0
15	39.201967804206035	40.0	39.0	41.0	36.0	41.0
16	39.18761604290505	40.0	39.0	41.0	36.0	41.0
17	39.17867276680301	40.0	39.0	41.0	36.0	41.0
18	39.16773957633533	40.0	39.0	41.0	36.0	41.0
19	39.199544190555564	40.0	39.0	41.0	36.0	41.0
20	39.16881390332191	40.0	39.0	41.0	36.0	41.0
21	39.14122722724425	40.0	39.0	41.0	36.0	41.0
22	39.1017719308689	40.0	39.0	41.0	36.0	41.0
23	39.05077116268259	40.0	39.0	41.0	36.0	41.0
24	39.0070072198175	40.0	38.0	41.0	36.0	41.0
25	38.949622851700354	40.0	38.0	41.0	36.0	41.0
26	38.86345275654163	40.0	38.0	41.0	35.0	41.0
27	38.78632798437549	40.0	38.0	41.0	35.0	41.0
28	38.720697660575034	40.0	38.0	41.0	35.0	41.0
29	38.66729028655334	40.0	38.0	41.0	35.0	41.0
30	38.5832291604659	40.0	38.0	41.0	35.0	41.0
31	38.51072484473282	40.0	38.0	41.0	34.0	41.0
32	38.422162316917955	40.0	38.0	41.0	34.0	41.0
33	38.35660569364956	40.0	38.0	41.0	34.0	41.0
34	38.28234957296211	40.0	38.0	41.0	34.0	41.0
35	38.262189642807535	40.0	38.0	41.0	34.0	41.0
36	38.237840120868874	40.0	38.0	41.0	34.0	41.0
37	38.1847785724207	40.0	38.0	41.0	34.0	41.0
38	38.14629555614138	40.0	38.0	41.0	34.0	41.0
39	38.10406231663449	40.0	38.0	41.0	34.0	41.0
40	38.04409559525936	40.0	37.0	41.0	33.0	41.0
41	38.01025287786405	40.0	37.0	41.0	33.0	41.0
42	37.98173360659223	40.0	37.0	41.0	33.0	41.0
43	37.095082190266425	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	1.0
14	0.0
15	0.0
16	1.0
17	4.0
18	6.0
19	16.0
20	34.0
21	56.0
22	97.0
23	150.0
24	289.0
25	410.0
26	621.0
27	945.0
28	1446.0
29	2034.0
30	2698.0
31	3771.0
32	5189.0
33	6959.0
34	10320.0
35	15011.0
36	24069.0
37	43035.0
38	103952.0
39	131664.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.39821531326978	18.202897564764342	12.486003985498002	26.912883136467876
2	19.42490907905516	20.5607476635514	33.314057809563494	26.70028544782995
3	20.14348926665136	21.44231941243668	30.05309272944251	28.361098591469446
4	15.896637838420089	15.592481411875422	33.17345987147761	35.33742087822688
5	16.203345437228407	34.652572857227895	33.29053033202089	15.853551373522802
6	35.587719223649934	33.74180435910301	15.332261840982596	15.33821457626446
7	30.368020772211494	29.335646396185716	20.405126155468437	19.891206676134352
8	27.834423250817085	32.13286505149116	19.91983649820426	20.112875199487497
9	27.190960913206286	14.150218692155711	18.711714699571118	39.94710569506688
10	17.628316878272233	26.147531457371333	32.287069241649874	23.937082422706567
11	37.16746178202217	20.694542475600873	20.583141286754596	21.55485445562236
12	22.889967940268555	24.42038783487679	27.598865011806257	25.090779213048396
13	31.785905623634058	18.641132266943327	23.29560433018972	26.27735777923289
14	24.079097678716703	19.770451188988005	23.307226337168597	32.843224795126694
15	26.57357722540174	26.313357654508913	20.852431692362643	26.26063342772671
16	26.37628657034574	25.027283370041868	22.418284535077202	26.17814552453519
17	25.133298750775985	25.167881308127747	23.98782240439482	25.710997536701445
18	24.8705280076195	24.055570201174106	25.00517321042352	26.068728580782867
19	26.11493314511351	24.169522562284033	24.674938134072608	25.040606158529844
20	26.531624614843853	24.02779076985875	24.234719186799666	25.205865428497727
21	26.49108932221022	24.308419718860815	23.302974383395835	25.897516575533125
22	26.80743468290346	24.169239098699187	23.28029729660779	25.743028921789563
23	26.037831050034153	24.13550693210197	23.531162569200546	26.295499448663328
24	26.408884882603555	24.041963949101277	23.48835956788811	26.06079160040705
25	26.29209788564512	23.54306803976427	23.883224341584956	26.281609733005652
26	25.8379892227145	23.84892524781804	23.984420841376615	26.328664688090846
27	25.898933893457375	23.88492512309406	23.85459451951505	26.36154646393351
28	25.58882473163085	24.127853415311	24.13550693210197	26.14781492095618
29	25.25320384716777	24.424639788649554	24.310970891124473	26.0111854730582
30	25.408825355250737	24.936291559304834	23.9030667925245	25.751816292919933
31	25.818713698944666	24.41897051695254	23.896830593657786	25.86548519044501
32	24.995251984953754	24.164703681341575	24.471127816565044	26.368916517139624
33	25.086243795690788	23.812358445372315	24.74580403028525	26.355593728651648
34	25.581171214839888	23.526627151842938	24.61172575465093	26.28047587866625
35	25.276731324710372	23.41465903582696	25.409675746005288	25.898933893457375
36	25.079157206069524	23.54788692070673	25.110905127572785	26.26205074565096
37	25.18857414982184	22.37264689791626	25.782997287253494	26.655781665008405
38	24.19049886756298	22.825054779337773	26.81225356384592	26.17219278925333
39	24.092420467204683	22.248206384166856	27.36245638204088	26.29691676658758
40	23.626973260880042	21.938947613094882	28.270957171486966	26.16312195453811
41	23.082723177966944	21.83775111330323	28.811522227797003	26.268003480932823
42	22.93277094158099	21.582633886937714	28.964592563616314	26.520002607864978
43	21.915420135552285	21.464429572055028	29.574889661799595	27.04526063059309
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	6.0
2	9.0
3	20.5
4	32.0
5	32.0
6	32.0
7	32.0
8	38.0
9	44.0
10	62.5
11	81.0
12	81.0
13	142.0
14	203.0
15	328.0
16	453.0
17	542.0
18	631.0
19	631.0
20	887.0
21	1143.0
22	1423.5
23	1704.0
24	2247.0
25	2790.0
26	2790.0
27	3297.5
28	3805.0
29	5003.5
30	6202.0
31	7132.5
32	8063.0
33	8063.0
34	9191.5
35	10320.0
36	11931.0
37	13542.0
38	14854.0
39	16166.0
40	16166.0
41	17706.5
42	19247.0
43	19706.0
44	20165.0
45	21360.0
46	22555.0
47	22555.0
48	23367.5
49	24180.0
50	25730.0
51	27280.0
52	28698.0
53	30116.0
54	30116.0
55	28741.0
56	27366.0
57	26495.5
58	25625.0
59	23540.0
60	21455.0
61	21455.0
62	19992.0
63	18529.0
64	16860.0
65	15191.0
66	13204.0
67	11217.0
68	11217.0
69	9728.5
70	8240.0
71	7211.5
72	6183.0
73	5162.5
74	4142.0
75	4142.0
76	3307.0
77	2472.0
78	2043.5
79	1615.0
80	1268.0
81	921.0
82	921.0
83	724.5
84	528.0
85	409.0
86	290.0
87	215.5
88	141.0
89	141.0
90	106.5
91	72.0
92	44.0
93	16.0
94	11.5
95	7.0
96	7.0
97	5.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	352779.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.58714865659785
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.20042958454165	68.93580428552335
2	5.195877858874217	7.854831842405016
3	1.3285254542226035	3.0125835300719435
4	0.6059979991607128	1.8322264339264667
5	0.33496299039719185	1.265944867480555
6	0.2257680676939914	1.0239098696819147
7	0.15914478991297012	0.8420510625152295
8	0.12163828009816441	0.7355432608090273
9	0.10099534850020449	0.6870555378638876
>10	0.6598047568660318	9.664015571682908
>50	0.05258248181249046	2.6706203351354434
>100	0.014272387919774344	1.475413402904281
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	2.834635848505722E-4
5	0.0	0.0	0.0	0.0	2.834635848505722E-4
6	0.0	0.0	0.0	0.0	2.834635848505722E-4
7	0.0	0.0	0.0	0.0	2.834635848505722E-4
8	0.0	0.0	0.0	0.0	2.834635848505722E-4
9	0.0	0.0	0.0	0.0	2.834635848505722E-4
10	0.0	0.0	0.0	0.0	5.669271697011444E-4
11	0.0	0.0	0.0	0.0	5.669271697011444E-4
12	0.0	0.0	0.0	0.0	5.669271697011444E-4
13	0.0	0.0	0.0	0.0	5.669271697011444E-4
14	0.0	0.0	0.0	0.0	5.669271697011444E-4
15	0.0	0.0	0.0	0.0	5.669271697011444E-4
16	0.0	0.0	0.0	0.0	5.669271697011444E-4
17	0.0	0.0	0.0	2.834635848505722E-4	5.669271697011444E-4
18	0.0	0.0	0.0	2.834635848505722E-4	5.669271697011444E-4
19	0.0	0.0	0.0	2.834635848505722E-4	5.669271697011444E-4
20	0.0	0.0	0.0	5.669271697011444E-4	5.669271697011444E-4
21	0.0	0.0	0.0	5.669271697011444E-4	5.669271697011444E-4
22	0.0	0.0	0.0	8.503907545517165E-4	5.669271697011444E-4
23	0.0	0.0	0.0	0.001984245093954005	5.669271697011444E-4
24	0.0	0.0	0.0	0.0028346358485057217	5.669271697011444E-4
25	0.0	0.0	0.0	0.00396849018790801	5.669271697011444E-4
26	0.0	0.0	0.0	0.007086589621264305	5.669271697011444E-4
27	0.0	0.0	0.0	0.0325983122578158	5.669271697011444E-4
28	0.0	0.0	0.0	0.10516498997956228	5.669271697011444E-4
29	0.0	0.0	0.0	0.20607802618636598	5.669271697011444E-4
30	0.0	0.0	0.0	0.35121138162985893	5.669271697011444E-4
31	0.0	0.0	0.0	0.7755563681511655	5.669271697011444E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	145	0.0	26.793102	1
TAGAGTG	35	8.8571635E-4	26.42857	5
GCGGGTA	35	8.8571635E-4	26.42857	23
TATGCCG	55	1.8978157E-5	23.545454	33
ATATGAC	40	0.0019285494	23.125	3
AGAGTGT	50	2.69696E-4	22.2	6
TATCAGT	45	0.0038206326	20.555555	6
CCCTAGA	45	0.0038206326	20.555555	1
ATATGTG	45	0.0038206326	20.555555	5
CCTAAGC	45	0.0038206326	20.555555	3
GAGCCGT	45	0.0038206326	20.555555	6
GCCGTCT	65	6.8848116E-5	19.923077	36
AGGTCGC	85	1.240307E-6	19.588236	10
TATATCA	70	1.21616E-4	18.5	2
AGAGGTC	120	5.136826E-9	18.5	8
GTATCAA	220	0.0	17.65909	2
TCGCCAT	105	4.7740104E-7	17.619047	13
CCGTCTT	85	2.7144613E-5	17.411764	37
TCTTATA	565	0.0	17.026548	37
CTTATAC	340	0.0	16.867647	37
>>END_MODULE