FastQCFastQC Report
Fri 10 Feb 2017
ERR1632292.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632292.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9720
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT1691.7386831275720165Illumina PCR Primer Index 10 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGT1021.0493827160493827No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT710.7304526748971194No Hit
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC670.6893004115226338No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATG660.6790123456790124No Hit
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC580.5967078189300412No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT540.5555555555555556No Hit
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTC390.4012345679012346Illumina PCR Primer Index 10 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT300.30864197530864196No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.22633744855967078No Hit
GTTCCGACCTGCACGAATGGCGTAACGATGGCCACACTGTCTC220.22633744855967078No Hit
CACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCG200.205761316872428No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC180.1851851851851852No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA160.1646090534979424No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT140.1440329218106996No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.1337448559670782No Hit
GGTAAGTTCCGACCTGCACGAATGGCGTAACGATGGCCACACT130.1337448559670782No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT130.1337448559670782No Hit
GTCTCAGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGC120.12345679012345678No Hit
GGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCG120.12345679012345678No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA120.12345679012345678No Hit
GTGCTGGAAGATTAAATGATGGGGTGCAAGCTCTTGATTGAAG120.12345679012345678No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG120.12345679012345678No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA120.12345679012345678No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC110.11316872427983539No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT110.11316872427983539No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACTGGGACTTC110.11316872427983539No Hit
CGTATAGGGTGTGACGCCTGCCCGGTGCTGGAAGATTAAATGA110.11316872427983539No Hit
GGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCG100.102880658436214No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTG100.102880658436214No Hit
GCATGAATGGGTTGCAAAAAATCGGTGGCTGCGACTGTTTATT100.102880658436214No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGATT200.001753438437.015
CAGTCGG200.001753438437.010
GGGCAGT200.001753438437.07
TCGGTGA200.001753438437.013
TGATTCC200.001753438437.017
GCAGTCG200.001753438437.09
AGTCGGT200.001753438437.011
CGGTGAT200.001753438437.014
GTGATTC200.001753438437.016
TGGGCAG200.001753438437.06
ATTCCTC200.001753438437.019
GGCAGTC200.001753438437.08
GTCGGTG200.001753438437.012
GGTATCA401.3948502E-632.3751
TTATTGA250.005235229.59999829
CTTATTG250.005235229.59999828
GATTCCT250.005235229.59999818
CCGTCTT405.3646305E-527.7537
GCCGTCT405.3646305E-527.7536
GTATCAA603.260708E-521.5833322
Produced by FastQC (version 0.11.5)