##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632292.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9720
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.47582304526749	33.0	31.0	34.0	31.0	34.0
2	32.6179012345679	34.0	31.0	34.0	31.0	34.0
3	32.66152263374486	34.0	31.0	34.0	31.0	34.0
4	36.19578189300412	37.0	35.0	37.0	35.0	37.0
5	36.059567901234566	37.0	35.0	37.0	35.0	37.0
6	36.08189300411522	37.0	35.0	37.0	35.0	37.0
7	36.053086419753086	37.0	35.0	37.0	35.0	37.0
8	36.05833333333333	37.0	35.0	37.0	35.0	37.0
9	37.77355967078189	39.0	37.0	39.0	35.0	39.0
10	37.71296296296296	39.0	37.0	39.0	35.0	39.0
11	37.790946502057615	39.0	37.0	39.0	35.0	39.0
12	37.711934156378604	39.0	37.0	39.0	35.0	39.0
13	37.80493827160494	39.0	37.0	39.0	35.0	39.0
14	38.98127572016461	40.0	38.0	41.0	36.0	41.0
15	39.00236625514403	40.0	38.0	41.0	36.0	41.0
16	39.007921810699585	40.0	38.0	41.0	36.0	41.0
17	38.98497942386831	40.0	38.0	41.0	36.0	41.0
18	39.03559670781893	40.0	38.0	41.0	36.0	41.0
19	39.074382716049385	40.0	39.0	41.0	36.0	41.0
20	39.08837448559671	40.0	39.0	41.0	36.0	41.0
21	39.0787037037037	40.0	39.0	41.0	36.0	41.0
22	39.02232510288066	40.0	38.0	41.0	36.0	41.0
23	39.01358024691358	40.0	38.0	41.0	36.0	41.0
24	38.906172839506176	40.0	38.0	41.0	35.0	41.0
25	38.877263374485594	40.0	38.0	41.0	35.0	41.0
26	38.72818930041152	40.0	38.0	41.0	35.0	41.0
27	38.56923868312757	40.0	38.0	41.0	35.0	41.0
28	38.5488683127572	40.0	38.0	41.0	35.0	41.0
29	38.492181069958846	40.0	38.0	41.0	35.0	41.0
30	38.3761316872428	40.0	38.0	41.0	34.0	41.0
31	38.255041152263374	40.0	38.0	41.0	34.0	41.0
32	38.1309670781893	40.0	37.0	41.0	34.0	41.0
33	37.961831275720165	40.0	37.0	41.0	33.0	41.0
34	37.85699588477366	40.0	37.0	41.0	33.0	41.0
35	37.82520576131687	40.0	37.0	41.0	33.0	41.0
36	37.77098765432099	40.0	37.0	41.0	33.0	41.0
37	37.675205761316874	40.0	37.0	41.0	33.0	41.0
38	37.637962962962966	40.0	37.0	41.0	33.0	41.0
39	37.49094650205761	40.0	36.0	41.0	33.0	41.0
40	37.297942386831274	39.0	36.0	41.0	32.0	41.0
41	37.18477366255144	39.0	36.0	41.0	32.0	41.0
42	37.04927983539095	39.0	35.0	41.0	32.0	41.0
43	36.163065843621396	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	0.0
21	2.0
22	0.0
23	6.0
24	6.0
25	12.0
26	26.0
27	33.0
28	47.0
29	82.0
30	94.0
31	129.0
32	164.0
33	230.0
34	332.0
35	476.0
36	799.0
37	1399.0
38	2759.0
39	3122.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.93621399176955	17.63374485596708	15.37037037037037	26.059670781893
2	20.061728395061728	20.09259259259259	33.01440329218107	26.83127572016461
3	19.166666666666668	22.078189300411523	33.7037037037037	25.051440329218106
4	14.722222222222223	17.58230452674897	30.648148148148145	37.047325102880656
5	15.20576131687243	34.825102880658434	32.03703703703704	17.9320987654321
6	32.55144032921811	32.34567901234568	16.33744855967078	18.765432098765434
7	27.067901234567906	32.19135802469136	18.641975308641975	22.098765432098766
8	30.843621399176957	30.29835390946502	18.919753086419753	19.938271604938272
9	26.810699588477366	12.602880658436213	17.530864197530864	43.05555555555556
10	20.432098765432098	24.372427983539094	26.75925925925926	28.43621399176955
11	35.56584362139918	21.64609053497942	17.551440329218106	25.236625514403293
12	21.54320987654321	28.189300411522634	24.022633744855966	26.24485596707819
13	35.46296296296296	16.862139917695472	23.106995884773664	24.567901234567902
14	24.25925925925926	21.54320987654321	22.026748971193417	32.17078189300411
15	29.886831275720166	25.08230452674897	19.279835390946502	25.751028806584365
16	28.08641975308642	23.220164609053498	22.05761316872428	26.6358024691358
17	21.903292181069958	29.845679012345677	22.273662551440328	25.977366255144034
18	24.609053497942387	22.314814814814817	24.382716049382715	28.69341563786008
19	24.403292181069958	28.06584362139918	24.372427983539094	23.15843621399177
20	25.833333333333336	21.32716049382716	25.87448559670782	26.96502057613169
21	29.72222222222222	20.627572016460903	24.969135802469136	24.681069958847736
22	28.230452674897123	22.726337448559672	22.232510288065843	26.810699588477366
23	27.29423868312757	22.00617283950617	23.508230452674898	27.191358024691358
24	25.267489711934154	23.065843621399175	24.691358024691358	26.97530864197531
25	24.578189300411523	23.569958847736626	25.092592592592595	26.75925925925926
26	26.38888888888889	23.51851851851852	23.53909465020576	26.553497942386834
27	24.506172839506174	24.012345679012345	24.567901234567902	26.913580246913583
28	24.362139917695476	22.890946502057613	26.224279835390945	26.522633744855966
29	23.590534979423868	23.281893004115226	26.03909465020576	27.08847736625514
30	23.569958847736626	22.829218106995885	28.51851851851852	25.08230452674897
31	23.631687242798353	23.220164609053498	25.545267489711932	27.602880658436213
32	24.547325102880656	22.674897119341566	27.41769547325103	25.36008230452675
33	23.827160493827158	23.15843621399177	27.34567901234568	25.66872427983539
34	24.72222222222222	24.4238683127572	24.609053497942387	26.24485596707819
35	23.44650205761317	22.633744855967077	29.835390946502056	24.084362139917694
36	24.753086419753085	24.310699588477366	23.96090534979424	26.97530864197531
37	22.283950617283953	23.909465020576132	27.26337448559671	26.543209876543212
38	23.32304526748971	21.59465020576132	27.15020576131687	27.9320987654321
39	23.713991769547324	21.54320987654321	28.641975308641975	26.10082304526749
40	22.469135802469136	19.34156378600823	31.954732510288064	26.234567901234566
41	21.563786008230455	21.450617283950617	29.475308641975307	27.510288065843618
42	20.493827160493826	20.246913580246915	33.26131687242798	25.997942386831273
43	20.195473251028805	20.88477366255144	32.27366255144033	26.64609053497942
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	2.5
4	4.0
5	4.0
6	3.5
7	3.0
8	7.0
9	11.0
10	13.0
11	15.0
12	15.0
13	32.0
14	49.0
15	87.5
16	126.0
17	102.0
18	78.0
19	78.0
20	87.0
21	96.0
22	64.0
23	32.0
24	42.0
25	52.0
26	52.0
27	60.5
28	69.0
29	82.0
30	95.0
31	98.5
32	102.0
33	102.0
34	105.5
35	109.0
36	138.0
37	167.0
38	218.0
39	269.0
40	269.0
41	293.5
42	318.0
43	425.5
44	533.0
45	636.5
46	740.0
47	740.0
48	862.0
49	984.0
50	1051.5
51	1119.0
52	986.0
53	853.0
54	853.0
55	893.0
56	933.0
57	872.0
58	811.0
59	670.0
60	529.0
61	529.0
62	487.0
63	445.0
64	391.0
65	337.0
66	301.5
67	266.0
68	266.0
69	247.0
70	228.0
71	181.5
72	135.0
73	114.0
74	93.0
75	93.0
76	76.5
77	60.0
78	46.5
79	33.0
80	24.5
81	16.0
82	16.0
83	10.5
84	5.0
85	4.0
86	3.0
87	2.0
88	1.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	9720.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.06995884773663
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.84263959390863	75.26748971193416
2	4.555837563451777	7.386831275720164
3	1.015228426395939	2.4691358024691357
4	0.5456852791878173	1.7695473251028806
5	0.20304568527918782	0.823045267489712
6	0.24111675126903556	1.1728395061728396
7	0.08883248730964467	0.5041152263374485
8	0.03807106598984771	0.24691358024691357
9	0.07614213197969542	0.5555555555555556
>10	0.3045685279187817	3.7654320987654324
>50	0.06345177664974619	3.251028806584362
>100	0.025380710659898477	2.788065843621399
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT	169	1.7386831275720165	Illumina PCR Primer Index 10 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGT	102	1.0493827160493827	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	71	0.7304526748971194	No Hit
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC	67	0.6893004115226338	No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATG	66	0.6790123456790124	No Hit
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC	58	0.5967078189300412	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	54	0.5555555555555556	No Hit
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTC	39	0.4012345679012346	Illumina PCR Primer Index 10 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	30	0.30864197530864196	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22	0.22633744855967078	No Hit
GTTCCGACCTGCACGAATGGCGTAACGATGGCCACACTGTCTC	22	0.22633744855967078	No Hit
CACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCG	20	0.205761316872428	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	18	0.1851851851851852	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	16	0.1646090534979424	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	14	0.1440329218106996	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13	0.1337448559670782	No Hit
GGTAAGTTCCGACCTGCACGAATGGCGTAACGATGGCCACACT	13	0.1337448559670782	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	13	0.1337448559670782	No Hit
GTCTCAGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGC	12	0.12345679012345678	No Hit
GGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCG	12	0.12345679012345678	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	12	0.12345679012345678	No Hit
GTGCTGGAAGATTAAATGATGGGGTGCAAGCTCTTGATTGAAG	12	0.12345679012345678	No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG	12	0.12345679012345678	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	12	0.12345679012345678	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	11	0.11316872427983539	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT	11	0.11316872427983539	No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACTGGGACTTC	11	0.11316872427983539	No Hit
CGTATAGGGTGTGACGCCTGCCCGGTGCTGGAAGATTAAATGA	11	0.11316872427983539	No Hit
GGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCG	10	0.102880658436214	No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTG	10	0.102880658436214	No Hit
GCATGAATGGGTTGCAAAAAATCGGTGGCTGCGACTGTTTATT	10	0.102880658436214	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0102880658436214	0.0
18	0.0	0.0	0.0	0.0102880658436214	0.0
19	0.0	0.0	0.0	0.0205761316872428	0.0
20	0.0	0.0	0.0	0.0205761316872428	0.0
21	0.0	0.0	0.0	0.0205761316872428	0.0
22	0.0	0.0	0.0	0.0205761316872428	0.0
23	0.0	0.0	0.0	0.0205761316872428	0.0
24	0.0	0.0	0.0	0.0205761316872428	0.0
25	0.0	0.0	0.0	0.0205761316872428	0.0
26	0.0	0.0	0.0	0.0411522633744856	0.0
27	0.0	0.0	0.0	0.0720164609053498	0.0
28	0.0	0.0	0.0	0.1440329218106996	0.0
29	0.0	0.0	0.0	0.2880658436213992	0.0
30	0.0	0.0	0.0	0.5967078189300411	0.0
31	0.0	0.0	0.0	1.0905349794238683	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTGATT	20	0.0017534384	37.0	15
CAGTCGG	20	0.0017534384	37.0	10
GGGCAGT	20	0.0017534384	37.0	7
TCGGTGA	20	0.0017534384	37.0	13
TGATTCC	20	0.0017534384	37.0	17
GCAGTCG	20	0.0017534384	37.0	9
AGTCGGT	20	0.0017534384	37.0	11
CGGTGAT	20	0.0017534384	37.0	14
GTGATTC	20	0.0017534384	37.0	16
TGGGCAG	20	0.0017534384	37.0	6
ATTCCTC	20	0.0017534384	37.0	19
GGCAGTC	20	0.0017534384	37.0	8
GTCGGTG	20	0.0017534384	37.0	12
GGTATCA	40	1.3948502E-6	32.375	1
TTATTGA	25	0.0052352	29.599998	29
CTTATTG	25	0.0052352	29.599998	28
GATTCCT	25	0.0052352	29.599998	18
CCGTCTT	40	5.3646305E-5	27.75	37
GCCGTCT	40	5.3646305E-5	27.75	36
GTATCAA	60	3.260708E-5	21.583332	2
>>END_MODULE