FastQCFastQC Report
Fri 10 Feb 2017
ERR1632291.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632291.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423928
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5570.13139023607782455No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5240.12360589534071824No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1950.028.461541
TACACTC358.8601245E-426.428575
TTATAAC502.6982045E-422.22
GTTCTAG603.7191494E-521.5833321
ATTAGAC450.003821884320.5555573
GTCTAAA450.003821884320.5555571
TAACCCG450.003821884320.55555728
CCAGTAC450.003821884320.5555573
TGCCCCG609.223713E-418.55
TAAGGGG500.00702773618.54
CGATCTT500.00702773618.529
TATGCCG953.5955345E-617.52631633
TCGTATG953.5955345E-617.52631630
GGATAAG650.001577710617.0769231
TGTAGGT650.001577710617.0769233
CGTATGC1005.8602E-616.65000231
TAGAACT803.3762687E-416.18754
GTATCAA3450.016.0869562
CTCGTAT1059.315221E-615.85714229
ATTAAGA700.00258887615.8571422
Produced by FastQC (version 0.11.5)