##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632291.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	423928
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.01717744522655	33.0	31.0	34.0	30.0	34.0
2	32.219185805136725	33.0	31.0	34.0	30.0	34.0
3	32.30570285520183	34.0	31.0	34.0	30.0	34.0
4	35.959684191655185	37.0	35.0	37.0	35.0	37.0
5	35.555325432620634	37.0	35.0	37.0	33.0	37.0
6	35.54454294125418	37.0	35.0	37.0	33.0	37.0
7	35.518823951237	37.0	35.0	37.0	33.0	37.0
8	35.48379205902889	37.0	35.0	37.0	33.0	37.0
9	37.17782500802023	39.0	37.0	39.0	34.0	39.0
10	37.02792927100828	39.0	37.0	39.0	33.0	39.0
11	37.13125577928327	39.0	37.0	39.0	33.0	39.0
12	37.07114415655489	39.0	37.0	39.0	33.0	39.0
13	37.121775395821935	39.0	37.0	39.0	33.0	39.0
14	38.29756939857712	40.0	38.0	41.0	34.0	41.0
15	38.27595487913042	40.0	38.0	41.0	34.0	41.0
16	38.25775131626125	40.0	38.0	41.0	34.0	41.0
17	38.24161414202412	40.0	38.0	41.0	34.0	41.0
18	38.22653610990545	40.0	38.0	41.0	34.0	41.0
19	38.27650685965541	40.0	38.0	41.0	34.0	41.0
20	38.243055424506046	40.0	38.0	41.0	34.0	41.0
21	38.21424392821422	40.0	38.0	41.0	34.0	41.0
22	38.17568077598083	40.0	38.0	41.0	34.0	41.0
23	38.11490158706195	40.0	38.0	41.0	34.0	41.0
24	38.046757940027554	40.0	37.0	41.0	33.0	41.0
25	37.97720839387821	40.0	37.0	41.0	33.0	41.0
26	37.86294606631315	39.0	37.0	41.0	33.0	41.0
27	37.75768998509181	39.0	37.0	40.0	33.0	41.0
28	37.68842822366062	39.0	37.0	40.0	33.0	41.0
29	37.606076975335434	39.0	37.0	40.0	33.0	41.0
30	37.515207771130946	39.0	37.0	40.0	32.0	41.0
31	37.42186173123738	39.0	37.0	40.0	32.0	41.0
32	37.31591921269649	39.0	36.0	40.0	32.0	41.0
33	37.22618935290898	39.0	36.0	40.0	32.0	41.0
34	37.14595402992961	39.0	36.0	40.0	31.0	41.0
35	37.12864684569078	39.0	36.0	40.0	31.0	41.0
36	37.075338264988396	39.0	36.0	40.0	31.0	41.0
37	37.02782312090732	39.0	36.0	40.0	31.0	41.0
38	36.96819978864335	39.0	36.0	40.0	31.0	41.0
39	36.91502566473552	39.0	36.0	40.0	31.0	41.0
40	36.843480496688116	39.0	36.0	40.0	31.0	41.0
41	36.806684153912926	39.0	35.0	40.0	31.0	41.0
42	36.764353852541	39.0	35.0	40.0	31.0	41.0
43	35.85230039063237	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	5.0
17	11.0
18	19.0
19	31.0
20	91.0
21	121.0
22	265.0
23	373.0
24	661.0
25	926.0
26	1437.0
27	2051.0
28	3020.0
29	4215.0
30	5783.0
31	7851.0
32	10335.0
33	14422.0
34	20681.0
35	30592.0
36	48447.0
37	80468.0
38	124409.0
39	67713.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.779736181615746	19.221424392821422	12.928374629654094	26.070464795908737
2	19.194768923024665	21.112783302825008	34.011435904210146	25.68101186994018
3	20.18031363816497	22.23773848389349	30.4768734313374	27.10507444660414
4	15.701251155856655	16.111934102017322	33.731435526787564	34.45537921533845
5	15.70361004698911	35.1946556962503	33.671047913796684	15.4306863429639
6	34.742456266158406	34.320214753448695	15.622228302919364	15.315100677473534
7	29.15801739918099	30.089307618274802	20.992243965956483	19.760431016587724
8	27.145175595855903	32.77018739031156	20.246834368100245	19.837802645732296
9	26.482327187635633	14.410465928176484	19.627153667603935	39.48005321658395
10	17.037091204166746	26.74109754486611	32.97281613858957	23.248995112377575
11	36.0969315544149	21.14887433715159	21.373204883848203	21.380989224585306
12	22.165556415240324	25.111339661451943	28.356702081485537	24.366401841822196
13	31.217801136041967	19.35069162688004	23.7974373006737	25.6340699364043
14	23.551640844671738	20.633456624709858	24.253882734804023	31.56101979581438
15	25.95134079371969	27.097761884093526	21.792143948972466	25.15875337321432
16	25.69775999698062	25.90581419486328	23.119020210979222	25.277405597176877
17	24.33196203128833	26.157507878696386	24.784869128719972	24.725660961295315
18	24.217084033137702	24.674001245494516	25.976109150610483	25.1328055707573
19	25.13256968164405	24.929469155139554	25.9291672170746	24.0087939461418
20	25.539242512879547	24.389283085807023	25.604112019022097	24.467362382291334
21	25.746589043422468	24.99080032458342	24.494725519427828	24.767885112566283
22	25.891189069842046	24.834405842501557	24.487177067803966	24.787228019852428
23	25.30877884923855	24.85799475382612	24.62257741880697	25.210648978128365
24	25.68667320865807	24.88347077805665	24.578466154630032	24.851389858655242
25	25.32717820007171	24.347058934536054	24.925694929327623	25.400067936064612
26	25.06534128436904	24.850682191315506	24.77637712064313	25.30759940367232
27	25.135636240116245	24.740286086316544	24.836292955407522	25.28778471815969
28	24.75844954803646	24.892434564359984	25.303589288747148	25.04552659885641
29	24.714810062086016	25.059679945651148	25.208761865222396	25.01674812704044
30	24.589788832065825	25.57368232341341	24.976411088675434	24.86011775584533
31	24.975467532222453	25.089637863033342	24.876865882885774	25.058028721858427
32	24.42891245683229	24.991272102809912	25.2382480043781	25.341567435979695
33	24.320403464739297	24.712687060066806	25.679596535260707	25.287312939933198
34	24.920269479722972	24.115179936215583	25.601989017002886	25.362561567058556
35	24.489535958936422	24.151270970542168	26.244786850597272	25.114406219924142
36	24.321582910305523	24.234775716631127	26.007954180898647	25.435687192164707
37	24.591911834085035	23.225642090166254	26.313194693438508	25.869251382310203
38	23.721716895321848	23.486299560302694	27.616010265894207	25.17597327848125
39	23.64316582061105	23.01404955558491	28.05830235322979	25.284482270574248
40	23.106046309750713	22.847040063406993	28.927789624653244	25.119124002189054
41	22.516087637523352	22.950831273235078	29.04856485063501	25.48451623860656
42	22.51514408107037	22.64464720424223	29.123105810420636	25.717102904266763
43	21.718546545639825	22.468673925760978	29.655035760789566	26.157743767809627
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	19.0
1	21.0
2	23.0
3	49.5
4	76.0
5	76.0
6	109.5
7	143.0
8	153.5
9	164.0
10	212.0
11	260.0
12	260.0
13	471.0
14	682.0
15	1093.0
16	1504.0
17	1528.0
18	1552.0
19	1552.0
20	2007.0
21	2462.0
22	2827.0
23	3192.0
24	3897.5
25	4603.0
26	4603.0
27	5392.5
28	6182.0
29	7785.0
30	9388.0
31	10515.0
32	11642.0
33	11642.0
34	13061.0
35	14480.0
36	16233.5
37	17987.0
38	19630.0
39	21273.0
40	21273.0
41	23218.0
42	25163.0
43	25369.0
44	25575.0
45	26614.5
46	27654.0
47	27654.0
48	28207.5
49	28761.0
50	30131.0
51	31501.0
52	32619.0
53	33737.0
54	33737.0
55	32104.5
56	30472.0
57	29169.0
58	27866.0
59	25723.0
60	23580.0
61	23580.0
62	21915.0
63	20250.0
64	18233.0
65	16216.0
66	14145.5
67	12075.0
68	12075.0
69	10463.0
70	8851.0
71	7652.5
72	6454.0
73	5234.5
74	4015.0
75	4015.0
76	3232.0
77	2449.0
78	2040.0
79	1631.0
80	1297.5
81	964.0
82	964.0
83	750.0
84	536.0
85	416.0
86	296.0
87	217.5
88	139.0
89	139.0
90	112.0
91	85.0
92	52.0
93	19.0
94	12.0
95	5.0
96	5.0
97	3.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	423928.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.27839068242123
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.39879851984777	68.80354462881004
2	5.187109526281381	7.809545148638374
3	1.2690270010035674	2.865909311042637
4	0.5675112543935	1.7088533569961935
5	0.318133196738816	1.1974277536576083
6	0.21622808128061097	0.9766381187491298
7	0.15382428141553084	0.8105751046988727
8	0.09284577039610674	0.5591424141670802
9	0.07975576425802555	0.5403497022891662
>10	0.6380347110316203	9.399080079305785
>50	0.0614798849204022	3.085955492596644
>100	0.016624662671634965	1.9877246329098097
>500	6.273457611937721E-4	0.2552542561386914
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	557	0.13139023607782455	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	524	0.12360589534071824	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	2.3588911324564548E-4	0.0
13	0.0	0.0	0.0	2.3588911324564548E-4	0.0
14	0.0	0.0	0.0	4.7177822649129095E-4	0.0
15	0.0	0.0	0.0	4.7177822649129095E-4	0.0
16	0.0	0.0	0.0	4.7177822649129095E-4	0.0
17	0.0	0.0	0.0	7.076673397369364E-4	0.0
18	0.0	0.0	0.0	9.435564529825819E-4	0.0
19	0.0	0.0	0.0	9.435564529825819E-4	0.0
20	0.0	0.0	0.0	0.0011794455662282274	0.0
21	0.0	0.0	0.0	0.0014153346794738729	0.0
22	0.0	0.0	0.0	0.0016512237927195183	0.0
23	0.0	0.0	0.0	0.0023588911324564548	0.0
24	0.0	0.0	0.0	0.004246004038421619	0.0
25	0.0	0.0	0.0	0.0056613387178954915	0.0
26	0.0	0.0	0.0	0.008256118963597592	0.0
27	0.0	0.0	0.0	0.02453246777754713	0.0
28	0.0	0.0	0.0	0.08067407673001076	0.0
29	0.0	0.0	0.0	0.17125549621633862	0.0
30	0.0	0.0	0.0	0.31255307505048024	0.0
31	0.0	0.0	0.0	0.6918627691494782	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	195	0.0	28.46154	1
TACACTC	35	8.8601245E-4	26.42857	5
TTATAAC	50	2.6982045E-4	22.2	2
GTTCTAG	60	3.7191494E-5	21.583332	1
ATTAGAC	45	0.0038218843	20.555557	3
GTCTAAA	45	0.0038218843	20.555557	1
TAACCCG	45	0.0038218843	20.555557	28
CCAGTAC	45	0.0038218843	20.555557	3
TGCCCCG	60	9.223713E-4	18.5	5
TAAGGGG	50	0.007027736	18.5	4
CGATCTT	50	0.007027736	18.5	29
TATGCCG	95	3.5955345E-6	17.526316	33
TCGTATG	95	3.5955345E-6	17.526316	30
GGATAAG	65	0.0015777106	17.076923	1
TGTAGGT	65	0.0015777106	17.076923	3
CGTATGC	100	5.8602E-6	16.650002	31
TAGAACT	80	3.3762687E-4	16.1875	4
GTATCAA	345	0.0	16.086956	2
CTCGTAT	105	9.315221E-6	15.857142	29
ATTAAGA	70	0.002588876	15.857142	2
>>END_MODULE