FastQCFastQC Report
Fri 10 Feb 2017
ERR1632290.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632290.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1937434
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT39530.20403275672874532No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT29650.15303747121192257No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT29080.15009543550902896No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14750.027.8440671
TTAGACG502.7030296E-422.1999994
CTTATAC26500.019.33773637
GTATCAA22900.017.6113532
ACTGATC2150.017.2093038
TATACAC8050.016.54658537
AATCTAC3850.015.85714414
TAGACTA700.002593353915.8571425
GTACGAC700.002593353915.8571423
TCTTATA45500.015.69450737
TTCTAGC1302.5914414E-715.6538462
ATACACA6150.015.64227637
GAATCTA3850.014.89610513
TATACTG1406.000464E-714.5357145
TAATACA3300.014.0151514
GGACCGT1203.3026536E-513.8750016
GTTAGAC800.006300678513.8753
TACCGAT800.006300678513.8757
ATCTACT4300.013.76744215
TAGAACT3650.013.6849324
Produced by FastQC (version 0.11.5)