##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632290.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1937434
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.747038092652446	34.0	31.0	34.0	31.0	34.0
2	32.915439183992845	34.0	31.0	34.0	31.0	34.0
3	33.000834092929104	34.0	33.0	34.0	31.0	34.0
4	36.40013904989796	37.0	37.0	37.0	35.0	37.0
5	36.268010678041165	37.0	37.0	37.0	35.0	37.0
6	36.29435944656695	37.0	37.0	37.0	35.0	37.0
7	36.27149982915547	37.0	37.0	37.0	35.0	37.0
8	36.25796181960263	37.0	37.0	37.0	35.0	37.0
9	38.04894257043079	39.0	38.0	39.0	35.0	39.0
10	38.010738946462176	39.0	38.0	39.0	35.0	39.0
11	38.07923779597137	39.0	38.0	39.0	36.0	39.0
12	38.04757891107516	39.0	38.0	39.0	35.0	39.0
13	38.0594487347698	39.0	38.0	39.0	35.0	39.0
14	39.44724671911405	40.0	39.0	41.0	37.0	41.0
15	39.454158954576	40.0	39.0	41.0	37.0	41.0
16	39.4588935674712	40.0	39.0	41.0	37.0	41.0
17	39.44633107501985	40.0	39.0	41.0	37.0	41.0
18	39.4445973385416	40.0	39.0	41.0	37.0	41.0
19	39.46370405391874	40.0	39.0	41.0	37.0	41.0
20	39.435549288388664	40.0	39.0	41.0	37.0	41.0
21	39.405488393411076	40.0	39.0	41.0	37.0	41.0
22	39.37648353440685	40.0	39.0	41.0	37.0	41.0
23	39.319478753856906	40.0	39.0	41.0	36.0	41.0
24	39.26968763839181	40.0	39.0	41.0	36.0	41.0
25	39.21169288863518	40.0	39.0	41.0	36.0	41.0
26	39.133063113375734	40.0	39.0	41.0	36.0	41.0
27	39.05751370111188	40.0	39.0	41.0	36.0	41.0
28	38.97810299602464	40.0	39.0	41.0	35.0	41.0
29	38.91732828060208	40.0	38.0	41.0	35.0	41.0
30	38.843789259401866	40.0	38.0	41.0	35.0	41.0
31	38.7698646766806	40.0	38.0	41.0	35.0	41.0
32	38.68392471691939	40.0	38.0	41.0	35.0	41.0
33	38.60009992598457	40.0	38.0	41.0	35.0	41.0
34	38.522383730232875	40.0	38.0	41.0	35.0	41.0
35	38.487971203148085	40.0	38.0	41.0	35.0	41.0
36	38.449303563373	40.0	38.0	41.0	34.0	41.0
37	38.397363213404944	40.0	38.0	41.0	34.0	41.0
38	38.34640818732406	40.0	38.0	41.0	34.0	41.0
39	38.292304150747846	40.0	38.0	41.0	34.0	41.0
40	38.215583601815595	40.0	38.0	41.0	34.0	41.0
41	38.15909290329374	40.0	37.0	41.0	34.0	41.0
42	38.09218481765056	40.0	37.0	41.0	34.0	41.0
43	37.20191449102266	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	1.0
13	3.0
14	2.0
15	0.0
16	4.0
17	6.0
18	34.0
19	46.0
20	102.0
21	205.0
22	372.0
23	742.0
24	1101.0
25	1850.0
26	2864.0
27	4238.0
28	6329.0
29	8878.0
30	12505.0
31	17243.0
32	23823.0
33	32785.0
34	48260.0
35	72418.0
36	117093.0
37	217576.0
38	503387.0
39	865565.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.29695876091779	17.299789308951944	12.037571344365796	27.36568058576447
2	19.902716686090987	19.520510118022084	33.0762751144039	27.500498081483034
3	20.529318676145873	20.559358409112257	28.61537476889535	30.29594814584652
4	15.726574427825671	14.895165461120225	32.157172837887636	37.22108727316646
5	16.916137530362324	33.91232940064023	32.224065439132374	16.947467629865066
6	36.7469549930475	32.41282025607066	15.008924174965443	15.831300575916393
7	31.58559207694301	28.331442516235395	19.75427291974849	20.32869248707311
8	28.865860720932947	31.294124083710724	19.00875074970296	20.831264445653375
9	27.925235130590252	13.465490953498287	18.537612119948342	40.07166179596312
10	18.470667904042152	25.232807930489503	30.803423497264937	25.493100668203404
11	38.329408898574094	20.068038446729027	19.883412802707085	21.719139851989798
12	23.997514237904362	23.56849317189644	27.210165610802743	25.223826979396456
13	32.63373100709495	17.872557207110024	22.80108638539429	26.692625400400736
14	24.636142444078097	18.858552084871018	23.08450249143971	33.420802979611175
15	27.170473936144408	25.65935149274762	20.481265426331944	26.68890914477603
16	27.369758144019357	23.62965654571975	22.063151570582534	26.937433739678358
17	25.712617823368433	24.51830617197799	23.47047692979477	26.298599074858807
18	26.360949585895572	22.73636159993063	24.551339555308722	26.351349258865074
19	26.671308545220125	23.292406347777526	24.005566125091228	26.03071898191113
20	26.97815770756578	23.115161600343548	23.94569311780427	25.960987574286403
21	27.88559507059337	22.942458943117547	23.5897584124156	25.582187573873483
22	27.714440853211	22.891618501585086	22.855230165259822	26.53871047994409
23	26.53907178257427	22.928213296556166	23.52916279986828	27.003552121001285
24	26.361465732510116	23.27594127077361	23.609113910460948	26.75347908625533
25	26.881380217339018	22.70002487826682	23.578661260202928	26.839933644191234
26	26.630120045379613	23.046720559255178	23.738150564096635	26.585008831268574
27	26.768447338077067	22.842584573203524	23.67765818087223	26.711309907847188
28	25.991595068528785	23.191964216587508	23.93444112160724	26.88199959327647
29	25.937864205954885	23.47651584518492	23.814127345757328	26.771492603102864
30	25.55854805892743	23.61344954202311	24.360210463943545	26.467791935105918
31	26.589705765460913	23.36373780990733	23.445288975005084	26.60126744962667
32	25.59937525613776	22.963208037022163	24.24010314673945	27.19731356010063
33	25.509978662498956	22.776001659927513	24.39541166305536	27.31860801451817
34	26.255552447205943	22.17577476187576	24.74690750755897	26.821765283359326
35	25.59700098171086	22.196833543749104	25.503217141848445	26.70294833269159
36	25.240756588353463	22.004827003139205	25.560509416062686	27.193906992444646
37	25.255673225513746	21.30131916751745	26.14829718070396	27.294710426264846
38	24.428754734354822	20.976095185694067	27.261935116241382	27.33321496370973
39	24.386121023993592	20.615308702128694	27.936848429417466	27.06172184446025
40	24.04179961743213	20.998031416812136	28.076879005942914	26.883289959812824
41	23.156814632137145	20.626509083664267	29.538502989005046	26.67817329519354
42	22.818996672918924	20.844684257631485	29.419531194352942	26.91678787509665
43	21.537972390285294	20.65190349709977	29.790021234271723	28.020102878343213
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	100.0
1	120.5
2	141.0
3	351.0
4	561.0
5	561.0
6	677.0
7	793.0
8	802.0
9	811.0
10	1147.0
11	1483.0
12	1483.0
13	2516.0
14	3549.0
15	5595.5
16	7642.0
17	7352.0
18	7062.0
19	7062.0
20	7813.0
21	8564.0
22	8185.5
23	7807.0
24	9405.5
25	11004.0
26	11004.0
27	13173.5
28	15343.0
29	18716.0
30	22089.0
31	25867.0
32	29645.0
33	29645.0
34	35632.5
35	41620.0
36	48587.0
37	55554.0
38	62990.0
39	70426.0
40	70426.0
41	78484.0
42	86542.0
43	93689.5
44	100837.0
45	111378.5
46	121920.0
47	121920.0
48	129401.5
49	136883.0
50	142254.5
51	147626.0
52	151629.0
53	155632.0
54	155632.0
55	157475.0
56	159318.0
57	154061.5
58	148805.0
59	147875.5
60	146946.0
61	146946.0
62	136090.0
63	125234.0
64	112947.5
65	100661.0
66	89644.5
67	78628.0
68	78628.0
69	66514.0
70	54400.0
71	46455.5
72	38511.0
73	29778.5
74	21046.0
75	21046.0
76	16462.0
77	11878.0
78	9595.0
79	7312.0
80	6066.0
81	4820.0
82	4820.0
83	3728.0
84	2636.0
85	2234.0
86	1832.0
87	1483.0
88	1134.0
89	1134.0
90	826.0
91	518.0
92	303.0
93	88.0
94	54.0
95	20.0
96	20.0
97	15.0
98	10.0
99	6.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1937434.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.420975000070506
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.14870683712698	47.190243529087475
2	7.880383110295399	8.734770306933184
3	2.3196691013605184	3.8567496982481195
4	1.206583324639564	2.67480097081405
5	0.7170532595933099	1.986989538681995
6	0.4859937402179658	1.6160548156086387
7	0.3400300989343888	1.3191359728619982
8	0.24452178984859102	1.0841308801737006
9	0.2143938031501013	1.069372224409662
>10	1.217078810849037	12.919722745292159
>50	0.1255160041299806	4.842429368591837
>100	0.0940031396669104	10.037681080529254
>500	0.005506951246918615	1.9831314720689737
>1k	5.60028940364605E-4	0.6847873966989871
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3953	0.20403275672874532	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2965	0.15303747121192257	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2908	0.15009543550902896	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	5.1614661454274055E-5	0.0
5	0.0	0.0	0.0	1.0322932290854811E-4	0.0
6	0.0	0.0	0.0	1.0322932290854811E-4	0.0
7	0.0	0.0	0.0	1.0322932290854811E-4	0.0
8	0.0	0.0	0.0	1.5484398436282218E-4	0.0
9	0.0	0.0	0.0	3.0968796872564436E-4	0.0
10	0.0	0.0	0.0	3.0968796872564436E-4	0.0
11	0.0	0.0	0.0	3.0968796872564436E-4	0.0
12	0.0	0.0	0.0	3.0968796872564436E-4	0.0
13	0.0	0.0	0.0	4.1291729163419244E-4	0.0
14	0.0	0.0	0.0	5.677612759970146E-4	0.0
15	0.0	0.0	0.0	6.709905989055627E-4	0.0
16	0.0	0.0	0.0	0.0011355225519940292	0.0
17	0.0	0.0	0.0	0.0015484398436282217	0.0
18	0.0	0.0	0.0	0.0017032838279910439	0.0
19	0.0	0.0	0.0	0.0022710451039880584	0.0
20	0.0	0.0	0.0	0.0025807330727137027	0.0
21	0.0	0.0	0.0	0.0032517236716192656	0.0
22	0.0	0.0	0.0	0.004490475546521842	0.0
23	0.0	5.1614661454274055E-5	0.0	0.006142144713058613	0.0
24	0.0	5.1614661454274055E-5	0.0	0.008722877785772316	0.0
25	0.0	5.1614661454274055E-5	0.0	0.010890693566851826	0.0
26	0.0	5.1614661454274055E-5	0.0	0.015845701066462135	0.0
27	0.0	5.1614661454274055E-5	0.0	0.04975653364192019	0.0
28	0.0	5.1614661454274055E-5	0.0	0.17198005196564115	0.0
29	5.1614661454274055E-5	5.1614661454274055E-5	0.0	0.3452504704676392	0.0
30	5.1614661454274055E-5	5.1614661454274055E-5	0.0	0.5848973435998336	0.0
31	5.1614661454274055E-5	5.1614661454274055E-5	0.0	1.2661592601347968	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1475	0.0	27.844067	1
TTAGACG	50	2.7030296E-4	22.199999	4
CTTATAC	2650	0.0	19.337736	37
GTATCAA	2290	0.0	17.611353	2
ACTGATC	215	0.0	17.209303	8
TATACAC	805	0.0	16.546585	37
AATCTAC	385	0.0	15.857144	14
TAGACTA	70	0.0025933539	15.857142	5
GTACGAC	70	0.0025933539	15.857142	3
TCTTATA	4550	0.0	15.694507	37
TTCTAGC	130	2.5914414E-7	15.653846	2
ATACACA	615	0.0	15.642276	37
GAATCTA	385	0.0	14.896105	13
TATACTG	140	6.000464E-7	14.535714	5
TAATACA	330	0.0	14.015151	4
GGACCGT	120	3.3026536E-5	13.875001	6
GTTAGAC	80	0.0063006785	13.875	3
TACCGAT	80	0.0063006785	13.875	7
ATCTACT	430	0.0	13.767442	15
TAGAACT	365	0.0	13.684932	4
>>END_MODULE