FastQCFastQC Report
Fri 10 Feb 2017
ERR1632289.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632289.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3091888
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT42130.13625978690043106No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT33610.10870380815863963No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17900.022.737431
CTTATAC28300.019.67667837
TCTTATA49100.015.9755637
CTAGACT2300.015.2826094
TAGCGTG855.3677935E-415.2352947
GTATCAA27250.014.732112
GTACTAT3650.014.698631
TTAATAC2151.2732926E-1114.6279073
GTATAAC1651.6481863E-814.5757571
GCGTTAT6100.013.950821
TAATACT3100.013.1290324
CGTATCT1000.001833698512.94999927
ATACCGG1000.001833698512.9499996
CTCTTAT76300.012.89908237
TAGATAA5600.012.8839294
GTACTAG1307.0052716E-512.8076931
CGTTATT5650.012.7699122
TATACAC9550.012.59162237
ATACCGT3700.012.5000016
TTATACA14150.012.42049437
Produced by FastQC (version 0.11.5)