##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632289.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3091888
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.57148480151933	34.0	31.0	34.0	31.0	34.0
2	32.74873507707912	34.0	31.0	34.0	31.0	34.0
3	32.83713737366942	34.0	31.0	34.0	31.0	34.0
4	36.29111533147385	37.0	37.0	37.0	35.0	37.0
5	36.130725951263436	37.0	35.0	37.0	35.0	37.0
6	36.14891419094094	37.0	36.0	37.0	35.0	37.0
7	36.12264803899753	37.0	35.0	37.0	35.0	37.0
8	36.109592908928136	37.0	35.0	37.0	35.0	37.0
9	37.87014536102213	39.0	38.0	39.0	35.0	39.0
10	37.810852786388125	39.0	37.0	39.0	35.0	39.0
11	37.886518851911845	39.0	38.0	39.0	35.0	39.0
12	37.84031375004528	39.0	38.0	39.0	35.0	39.0
13	37.87016282607908	39.0	38.0	39.0	35.0	39.0
14	39.22196826016984	40.0	39.0	41.0	36.0	41.0
15	39.22106557546716	40.0	39.0	41.0	36.0	41.0
16	39.21887597480892	40.0	39.0	41.0	36.0	41.0
17	39.201080052058806	40.0	39.0	41.0	36.0	41.0
18	39.190782783852455	40.0	39.0	41.0	36.0	41.0
19	39.21300609853915	40.0	39.0	41.0	36.0	41.0
20	39.18623604736006	40.0	39.0	41.0	36.0	41.0
21	39.155532800670656	40.0	39.0	41.0	36.0	41.0
22	39.11640880911598	40.0	39.0	41.0	36.0	41.0
23	39.06505377943832	40.0	39.0	41.0	36.0	41.0
24	39.01674316792846	40.0	39.0	41.0	36.0	41.0
25	38.9576491774605	40.0	38.0	41.0	35.0	41.0
26	38.86455977706825	40.0	38.0	41.0	35.0	41.0
27	38.78709901522953	40.0	38.0	41.0	35.0	41.0
28	38.710564871690046	40.0	38.0	41.0	35.0	41.0
29	38.64428756798435	40.0	38.0	41.0	35.0	41.0
30	38.57161449573853	40.0	38.0	41.0	35.0	41.0
31	38.49332155627888	40.0	38.0	41.0	34.0	41.0
32	38.40778061818539	40.0	38.0	41.0	34.0	41.0
33	38.32543772607546	40.0	38.0	41.0	34.0	41.0
34	38.25271969747934	40.0	38.0	41.0	34.0	41.0
35	38.21847557220701	40.0	38.0	41.0	34.0	41.0
36	38.185018668205316	40.0	38.0	41.0	34.0	41.0
37	38.126332195732836	40.0	38.0	41.0	34.0	41.0
38	38.07861054475453	40.0	37.0	41.0	33.0	41.0
39	38.03123366693748	40.0	37.0	41.0	33.0	41.0
40	37.953926856341496	40.0	37.0	41.0	33.0	41.0
41	37.90537787914698	40.0	37.0	41.0	33.0	41.0
42	37.84808505353364	40.0	37.0	41.0	33.0	41.0
43	36.963111535734804	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	3.0
12	2.0
13	2.0
14	2.0
15	5.0
16	15.0
17	20.0
18	57.0
19	128.0
20	240.0
21	454.0
22	908.0
23	1480.0
24	2344.0
25	3862.0
26	6029.0
27	8635.0
28	12641.0
29	17915.0
30	24788.0
31	33994.0
32	45424.0
33	62413.0
34	90763.0
35	133676.0
36	212124.0
37	381747.0
38	876375.0
39	1175839.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.2375299493384	17.54782191334227	11.895547316073545	27.31910082124579
2	19.742726774061673	19.583341958052813	32.6763776695663	27.997553598319215
3	20.258042982152006	20.833516608622304	28.707152393618397	30.201288015607293
4	15.48836827207195	14.93715166914196	32.704030676402255	36.87044938238383
5	16.98677313020394	33.75154598096697	32.502018184358555	16.75966270447054
6	36.76158386073493	32.97587105354398	14.954293299110446	15.308251786610642
7	31.61194713391947	28.535897807423815	19.966182474915005	19.885972583741715
8	28.97818420330879	31.393407523170307	18.9818001169512	20.646608156569705
9	27.64110472306888	13.551493456425328	18.348271347474423	40.459130473031365
10	18.249755489202713	24.966201880533838	31.2327289992393	25.551313631024154
11	38.769030443534824	19.982095082357446	20.1263111729791	21.12256330112863
12	23.766643552418458	23.60522114643221	27.246717863001507	25.381417438147825
13	32.13040058372101	17.844630853381492	22.828931707746207	27.196036855151284
14	24.26986359143669	18.930213513555472	22.970528039825506	33.829394855182336
15	27.05819227604622	25.655069006380565	20.31577469817794	26.97096401939527
16	27.195551714680484	24.02270069290996	22.22405856874505	26.557689023664505
17	25.988425195220525	24.255503433500824	23.47051380903836	26.285557562240285
18	26.25648147668997	23.050964329885172	24.351852331002934	26.340701862421923
19	26.754882453698194	23.464077612125667	23.87977831021046	25.90126162396568
20	26.869116863224026	23.125093793824355	23.507578541007952	26.498210801943667
21	27.650904560579164	23.291917430385578	23.144273013770228	25.91290499526503
22	27.676131865061087	23.06949669586997	23.040129526037166	26.214241913031778
23	26.710346558478186	22.955876797607157	23.47630315198998	26.857473491924676
24	26.52162691533458	23.415563565045048	23.25404413096464	26.808765388655736
25	26.71694446888115	22.600333517902328	23.57844139244371	27.10428062077281
26	26.44219324891458	23.437071459250788	23.625370647319695	26.495364644514936
27	26.61470920033326	23.054004543502224	23.41051810414866	26.920768152015857
28	26.294613517695336	23.253558990493833	23.835695212763206	26.616132279047623
29	25.97687885201534	23.549721076571984	24.04796034008994	26.425439731322736
30	25.768753590039484	23.83795920162697	23.83524241499045	26.558044793343093
31	26.60173977841371	23.479246337512873	23.255564237773164	26.66344964630025
32	25.651931764669357	23.422937700201302	23.6560638677727	27.269066667356647
33	25.47246213316912	23.013608513633095	24.507808821018095	27.00612053217969
34	26.393000005174834	22.578211112433568	24.31132693034159	26.71746195205001
35	26.015819460472052	22.791964003870774	24.977424796758484	26.21479173889869
36	25.473594127601	22.86893962523869	24.702414835207485	26.955051411952823
37	25.374431415368214	22.0073301490869	25.452733087356332	27.16550534818855
38	24.713281981753543	21.84542260262985	26.408395129448415	27.032900286168193
39	24.640025770661808	21.353231423647944	26.863521576460727	27.143221229229518
40	24.397973018427578	21.482634558560985	27.59847704703405	26.520915375977395
41	23.571520055060212	21.344660608663705	28.428034909414574	26.655784426861516
42	23.46343075816459	21.502137205487394	28.553783319447533	26.480648716900486
43	22.596743478418365	20.757123155819357	29.06596228582665	27.580171079935624
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	73.0
1	92.0
2	111.0
3	305.5
4	500.0
5	500.0
6	659.5
7	819.0
8	786.0
9	753.0
10	1107.0
11	1461.0
12	1461.0
13	2534.0
14	3607.0
15	5989.0
16	8371.0
17	8091.0
18	7811.0
19	7811.0
20	9150.0
21	10489.0
22	10358.5
23	10228.0
24	12773.5
25	15319.0
26	15319.0
27	19261.5
28	23204.0
29	28755.0
30	34306.0
31	42004.0
32	49702.0
33	49702.0
34	60557.0
35	71412.0
36	83855.5
37	96299.0
38	110459.5
39	124620.0
40	124620.0
41	137610.0
42	150600.0
43	162601.0
44	174602.0
45	186016.5
46	197431.0
47	197431.0
48	208589.0
49	219747.0
50	229027.5
51	238308.0
52	243870.0
53	249432.0
54	249432.0
55	247103.0
56	244774.0
57	239924.0
58	235074.0
59	228536.5
60	221999.0
61	221999.0
62	205001.0
63	188003.0
64	169710.0
65	151417.0
66	133935.0
67	116453.0
68	116453.0
69	100524.5
70	84596.0
71	72646.0
72	60696.0
73	48811.0
74	36926.0
75	36926.0
76	29997.5
77	23069.0
78	19346.0
79	15623.0
80	13190.0
81	10757.0
82	10757.0
83	8319.0
84	5881.0
85	4806.5
86	3732.0
87	3049.5
88	2367.0
89	2367.0
90	1715.5
91	1064.0
92	618.0
93	172.0
94	115.5
95	59.0
96	59.0
97	39.0
98	19.0
99	10.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3091888.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.7197681640561
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.80688682448964	48.373140780608296
2	8.968692362983775	10.353416878523278
3	2.676167132082368	4.634032392961835
4	1.2827174820873346	2.9615262274425094
5	0.7256973446345549	2.0943541244788815
6	0.4790172447950792	1.6589258589694122
7	0.3327502443200509	1.3444386871080451
8	0.24246381272162373	1.1195964046772189
9	0.18461748257810334	0.959047046409581
>10	1.1343450849848689	12.275216594158843
>50	0.0976687436202175	3.8950275083869896
>100	0.06236987328823491	7.0019512992248485
>500	0.00492678247869281	1.911307980107145
>1k	0.0016795849355139383	1.4180182169431244
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4213	0.13625978690043106	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3361	0.10870380815863963	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	3.2342698053745805E-5	0.0	0.0	0.0
2	0.0	6.468539610749161E-5	0.0	0.0	0.0
3	0.0	6.468539610749161E-5	0.0	0.0	0.0
4	0.0	6.468539610749161E-5	0.0	0.0	0.0
5	0.0	1.61713490268729E-4	0.0	0.0	0.0
6	0.0	1.61713490268729E-4	0.0	0.0	3.2342698053745805E-5
7	0.0	1.61713490268729E-4	0.0	0.0	3.2342698053745805E-5
8	0.0	1.61713490268729E-4	0.0	0.0	3.2342698053745805E-5
9	0.0	1.61713490268729E-4	0.0	0.0	3.2342698053745805E-5
10	0.0	1.9405618832247482E-4	0.0	3.2342698053745805E-5	3.2342698053745805E-5
11	0.0	2.910842824837122E-4	0.0	6.468539610749161E-5	3.2342698053745805E-5
12	0.0	2.910842824837122E-4	0.0	9.702809416123741E-5	3.2342698053745805E-5
13	0.0	2.910842824837122E-4	0.0	9.702809416123741E-5	3.2342698053745805E-5
14	0.0	2.910842824837122E-4	0.0	1.61713490268729E-4	3.2342698053745805E-5
15	0.0	2.910842824837122E-4	0.0	2.263988863762206E-4	3.2342698053745805E-5
16	0.0	3.23426980537458E-4	0.0	5.174831688599329E-4	6.468539610749161E-5
17	0.0	3.23426980537458E-4	0.0	8.08567451343645E-4	6.468539610749161E-5
18	0.0	3.557696785912038E-4	0.0	0.0010349663377198658	6.468539610749161E-5
19	0.0	3.557696785912038E-4	0.0	0.0012613652240960863	6.468539610749161E-5
20	0.0	3.557696785912038E-4	0.0	0.0014877641104723068	6.468539610749161E-5
21	0.0	3.557696785912038E-4	0.0	0.0020052472793322396	6.468539610749161E-5
22	0.0	3.557696785912038E-4	0.0	0.0025227304481921725	6.468539610749161E-5
23	0.0	3.557696785912038E-4	0.0	0.0035253540878582923	6.468539610749161E-5
24	0.0	3.557696785912038E-4	0.0	0.004722033915846887	6.468539610749161E-5
25	0.0	3.557696785912038E-4	0.0	0.006112769932157956	6.468539610749161E-5
26	0.0	3.557696785912038E-4	0.0	0.00908829815310257	6.468539610749161E-5
27	0.0	3.557696785912038E-4	0.0	0.029787624907499883	6.468539610749161E-5
28	0.0	3.8811237664494963E-4	0.0	0.11142059479515429	6.468539610749161E-5
29	0.0	3.8811237664494963E-4	0.0	0.22077125691486885	6.468539610749161E-5
30	0.0	3.8811237664494963E-4	0.0	0.365795914987865	6.468539610749161E-5
31	0.0	3.8811237664494963E-4	0.0	0.8347326940691254	6.468539610749161E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1790	0.0	22.73743	1
CTTATAC	2830	0.0	19.676678	37
TCTTATA	4910	0.0	15.97556	37
CTAGACT	230	0.0	15.282609	4
TAGCGTG	85	5.3677935E-4	15.235294	7
GTATCAA	2725	0.0	14.73211	2
GTACTAT	365	0.0	14.69863	1
TTAATAC	215	1.2732926E-11	14.627907	3
GTATAAC	165	1.6481863E-8	14.575757	1
GCGTTAT	610	0.0	13.95082	1
TAATACT	310	0.0	13.129032	4
CGTATCT	100	0.0018336985	12.949999	27
ATACCGG	100	0.0018336985	12.949999	6
CTCTTAT	7630	0.0	12.899082	37
TAGATAA	560	0.0	12.883929	4
GTACTAG	130	7.0052716E-5	12.807693	1
CGTTATT	565	0.0	12.769912	2
TATACAC	955	0.0	12.591622	37
ATACCGT	370	0.0	12.500001	6
TTATACA	1415	0.0	12.420494	37
>>END_MODULE