FastQCFastQC Report
Fri 10 Feb 2017
ERR1632288.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632288.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154782
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGCT358.834613E-426.4285711
GGTATCA802.687375E-823.1251
GCGGTCG450.003811089320.5555559
GTATCAA909.3459676E-820.5555552
CTGGACA801.6019907E-518.54
AAGCTGT803.3589668E-416.187537
TCAACGC1151.2252203E-616.0869565
CAACGCA1252.9187286E-614.7999996
ATCAACG1252.9187286E-614.7999994
TCTCTTA2550.014.50980437
CAGTTCT800.00626620313.8754
GGACAGC1203.2642387E-513.8749996
AACGCAG1356.4790056E-613.7037047
TATCAAC1409.431375E-613.2142853
CAGGCCT850.00935672413.0588244
TGTCTCT6000.012.9537
CTCTGTC1000.001820062312.94999936
CATGGGG1552.6842043E-511.9354844
TCTGTCT1757.218043E-611.628571537
ACGCAGA1603.7138485E-511.56258
Produced by FastQC (version 0.11.5)