##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632288.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	154782
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.562274683102686	31.0	31.0	34.0	30.0	34.0
2	31.771284774715408	31.0	31.0	34.0	30.0	34.0
3	31.862425863472495	31.0	31.0	34.0	30.0	34.0
4	35.626913982245995	37.0	35.0	37.0	33.0	37.0
5	34.88180796216614	37.0	35.0	37.0	32.0	37.0
6	34.83171815844219	36.0	35.0	37.0	32.0	37.0
7	34.81183858588208	35.0	35.0	37.0	32.0	37.0
8	34.7600237753744	35.0	35.0	37.0	32.0	37.0
9	36.41615304105129	38.0	35.0	39.0	32.0	39.0
10	36.120220697497125	37.0	35.0	39.0	32.0	39.0
11	36.283941285162356	38.0	35.0	39.0	32.0	39.0
12	36.18989288160122	37.0	35.0	39.0	32.0	39.0
13	36.30336214805339	38.0	35.0	39.0	32.0	39.0
14	37.31855125272965	39.0	36.0	40.0	32.0	41.0
15	37.27404995412903	39.0	36.0	40.0	32.0	41.0
16	37.26860358439612	39.0	36.0	40.0	32.0	41.0
17	37.20677468956339	39.0	36.0	40.0	32.0	41.0
18	37.18317375405409	38.0	36.0	40.0	32.0	41.0
19	37.1977878564691	39.0	36.0	40.0	32.0	41.0
20	37.155767466501274	39.0	36.0	40.0	32.0	41.0
21	37.146166866948356	39.0	36.0	40.0	32.0	41.0
22	37.07941491904744	39.0	36.0	40.0	32.0	41.0
23	37.010556783088475	39.0	36.0	40.0	31.0	41.0
24	36.927058701916245	38.0	36.0	40.0	31.0	41.0
25	36.81837035314184	38.0	36.0	40.0	31.0	41.0
26	36.663662441369155	38.0	36.0	40.0	31.0	41.0
27	36.5447403444845	38.0	35.0	40.0	30.0	41.0
28	36.43215619387267	38.0	35.0	40.0	30.0	41.0
29	36.32340323810262	38.0	35.0	40.0	30.0	41.0
30	36.19877634350247	38.0	35.0	40.0	30.0	41.0
31	36.089480688969005	38.0	35.0	40.0	30.0	41.0
32	35.95378661601478	38.0	35.0	40.0	29.0	41.0
33	35.82136165704022	38.0	34.0	40.0	29.0	41.0
34	35.72947112713364	38.0	34.0	40.0	29.0	41.0
35	35.71685338088408	38.0	34.0	40.0	29.0	41.0
36	35.65584499489605	38.0	34.0	40.0	28.0	41.0
37	35.589390239175096	38.0	34.0	40.0	28.0	41.0
38	35.50745564729749	38.0	34.0	40.0	28.0	41.0
39	35.42383481283353	38.0	34.0	40.0	27.0	41.0
40	35.330968717292706	38.0	34.0	40.0	27.0	41.0
41	35.26773785065447	38.0	33.0	40.0	27.0	41.0
42	35.20183871509607	38.0	33.0	40.0	27.0	41.0
43	34.201890400692584	37.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	1.0
16	6.0
17	12.0
18	15.0
19	29.0
20	46.0
21	107.0
22	204.0
23	272.0
24	475.0
25	662.0
26	1009.0
27	1381.0
28	2001.0
29	2769.0
30	3709.0
31	4843.0
32	6647.0
33	8960.0
34	12387.0
35	16882.0
36	22868.0
37	29124.0
38	29495.0
39	10877.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.99207918233387	18.417516248659403	11.863782610381051	26.726621958625678
2	21.389438048351874	20.27625951338011	31.35958961636366	26.974712821904355
3	22.038738354589036	21.22210592963006	28.168004031476528	28.571151684304375
4	16.872116912819322	15.59871302864674	31.710405602718662	35.81876445581528
5	17.463916992931992	33.560103888049	32.184620950756546	16.79135816826246
6	36.95132508948069	32.38619477717047	14.572753937796385	16.089726195552455
7	30.803969453812456	28.09176777661485	20.008786551407788	21.0954762181649
8	28.512359318266984	30.672817252652116	19.141114599888876	21.673708829192027
9	27.016061299117467	13.325192851882	18.73538266723521	40.92336318176532
10	18.382628471010843	24.936362109289195	31.90358051969867	24.77742890000129
11	38.19371761574343	20.098590275355015	19.942887415849388	21.764804693052163
12	23.218462095075655	23.54472742308537	27.48510808750371	25.751702394335258
13	32.32481813130726	18.365830652143014	22.2855370779548	27.023814138594926
14	24.198550219017715	19.73162254008864	22.71388145908439	33.355945781809254
15	27.1788709281441	25.84667467793413	20.349911488415966	26.62454290550581
16	27.10651109302115	24.33680918969906	22.210592963006036	26.346086754273752
17	25.836337558630852	24.520939127288703	23.367058185060277	26.27566512902017
18	26.005607887222027	23.59059838999367	24.23343799666628	26.17035572611802
19	26.510188523213294	23.84967244253208	23.84967244253208	25.79046659172255
20	26.545722370818314	23.41615950175085	23.850318512488535	26.187799614942307
21	27.08389864454523	23.514362135131993	23.406468452404024	25.99527076791875
22	27.288702820741428	23.51113178534972	23.056298536005478	26.143866857903376
23	26.77959969505498	23.111214482304142	23.42520448114122	26.683981341499656
24	26.905583336563684	23.646806476205242	23.03368608752956	26.413924099701514
25	26.904291196650775	23.149332609734984	23.23913633368221	26.707239859932034
26	26.87069555891512	23.780542957191404	23.251411662854853	26.097349821038623
27	26.846790970526293	23.341215386802084	23.149978679691436	26.66201496298019
28	26.177462495639027	23.584783760385577	23.615149048338953	26.622604695636443
29	25.924203072708714	23.786357586799497	23.919447997829206	26.369991342662587
30	26.085720561822434	23.84321174296753	23.910403018438835	26.1606646767712
31	26.78864467444535	23.500148596089986	23.127366231215515	26.58384049824915
32	26.245945911023245	23.56023310204029	23.558294892170924	26.635526094765545
33	25.995916837875203	23.490457546743162	23.989223553126333	26.524402062255305
34	26.403586980398234	23.142871910170435	23.799278985928595	26.654262123502733
35	26.3454406843173	22.93483738419196	24.395601555736455	26.324120375754283
36	25.90094455427634	23.370934604799007	23.819953224535155	26.9081676163895
37	26.146451137729194	22.473543435283172	24.658552028013595	26.72145339897404
38	25.32788050290085	22.430256748200694	25.482937292450025	26.758925456448424
39	25.196728301740514	22.385677921205307	25.88543887532142	26.532154901732756
40	25.042317582147795	22.173766975488103	26.307322556886458	26.47659288547764
41	24.730265793180088	22.16084557635901	26.485637864868007	26.6232507655929
42	24.64369241901513	22.332054114819552	26.43330619839516	26.590947267770154
43	24.123606104068948	22.03033944515512	26.856482019873113	26.989572430902818
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	1.5
2	3.0
3	7.0
4	11.0
5	11.0
6	18.0
7	25.0
8	25.5
9	26.0
10	32.0
11	38.0
12	38.0
13	71.0
14	104.0
15	148.0
16	192.0
17	199.0
18	206.0
19	206.0
20	305.0
21	404.0
22	467.0
23	530.0
24	669.5
25	809.0
26	809.0
27	1048.0
28	1287.0
29	1579.5
30	1872.0
31	2282.0
32	2692.0
33	2692.0
34	3184.0
35	3676.0
36	4313.0
37	4950.0
38	5621.0
39	6292.0
40	6292.0
41	6917.0
42	7542.0
43	8174.5
44	8807.0
45	9389.5
46	9972.0
47	9972.0
48	10644.5
49	11317.0
50	11540.0
51	11763.0
52	12017.0
53	12271.0
54	12271.0
55	12095.5
56	11920.0
57	11852.5
58	11785.0
59	11314.5
60	10844.0
61	10844.0
62	10096.0
63	9348.0
64	8500.5
65	7653.0
66	6718.5
67	5784.0
68	5784.0
69	4992.5
70	4201.0
71	3659.5
72	3118.0
73	2585.0
74	2052.0
75	2052.0
76	1663.0
77	1274.0
78	1064.0
79	854.0
80	696.0
81	538.0
82	538.0
83	421.0
84	304.0
85	239.5
86	175.0
87	131.5
88	88.0
89	88.0
90	63.0
91	38.0
92	27.0
93	16.0
94	8.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	154782.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.31121117988152
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.87788767639002	74.80269004112891
2	5.844376901133001	9.6211548264796
3	1.3785073130511667	3.403998196726969
4	0.6203156565558623	2.0423573201982577
5	0.3409442314471742	1.403176631760538
6	0.205169174204507	1.0132633935329445
7	0.15035328707206796	0.8663032814644837
8	0.12843899914324694	0.8457575665769937
9	0.07765943031442649	0.5753017591848048
>10	0.36937793846773626	4.993294107723645
>50	0.005973764760655368	0.3228881303746266
>100	9.95627460109228E-4	0.10981474484822325
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	6.460699564548849E-4	0.0
18	0.0	0.0	0.0	6.460699564548849E-4	0.0
19	0.0	0.0	0.0	6.460699564548849E-4	0.0
20	0.0	0.0	0.0	0.0012921399129097698	0.0
21	0.0	0.0	0.0	0.0012921399129097698	0.0
22	0.0	0.0	0.0	0.0025842798258195396	0.0
23	0.0	0.0	0.0	0.0032303497822744245	0.0
24	0.0	0.0	0.0	0.005814629608093964	0.0
25	0.0	0.0	0.0	0.007106769521003735	0.0
26	0.0	0.0	0.0	0.010983189259733043	0.0
27	0.0	0.0	0.0	0.035533847605018674	0.0
28	0.0	0.0	0.0	0.08269695442622527	0.0
29	0.0	0.0	0.0	0.13373648098616117	0.0
30	0.0	0.0	0.0	0.19511312684937526	0.0
31	0.0	0.0	0.0	0.359214895788916	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGCT	35	8.834613E-4	26.42857	11
GGTATCA	80	2.687375E-8	23.125	1
GCGGTCG	45	0.0038110893	20.555555	9
GTATCAA	90	9.3459676E-8	20.555555	2
CTGGACA	80	1.6019907E-5	18.5	4
AAGCTGT	80	3.3589668E-4	16.1875	37
TCAACGC	115	1.2252203E-6	16.086956	5
CAACGCA	125	2.9187286E-6	14.799999	6
ATCAACG	125	2.9187286E-6	14.799999	4
TCTCTTA	255	0.0	14.509804	37
CAGTTCT	80	0.006266203	13.875	4
GGACAGC	120	3.2642387E-5	13.874999	6
AACGCAG	135	6.4790056E-6	13.703704	7
TATCAAC	140	9.431375E-6	13.214285	3
CAGGCCT	85	0.009356724	13.058824	4
TGTCTCT	600	0.0	12.95	37
CTCTGTC	100	0.0018200623	12.949999	36
CATGGGG	155	2.6842043E-5	11.935484	4
TCTGTCT	175	7.218043E-6	11.6285715	37
ACGCAGA	160	3.7138485E-5	11.5625	8
>>END_MODULE