FastQCFastQC Report
Fri 10 Feb 2017
ERR1632287.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632287.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1089447
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT21520.1975314081364215No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18100.16613933491028018No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13770.12639440009472697No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAT250.005496219729.5999988
GGTATCA9100.020.3296721
CGTATAC500.007034653318.4999983
GCGTTAT1002.8745853E-718.4999981
CTTATAC11350.018.25550737
TAGCCCG852.7229042E-517.4117645
TATACAG852.7229042E-517.4117645
GTATTAC650.00157986217.0769231
AGTGCGT650.00157986217.0769238
CGCAATT650.00157986217.07692312
CGTTATT803.3823628E-416.18752
TAGAGTC1504.669346E-916.0333335
CTTATGC700.00259237815.8571423
TCTTATA19100.015.69109937
TATACTG1455.343827E-815.3103455
CCCTAGA855.3638313E-415.2352941
GTATCAA12050.015.1991712
TATGAAC1001.0932807E-414.7999993
ACTATGC1051.6555059E-414.0952388
CTAGAGT1854.8949005E-914.04
Produced by FastQC (version 0.11.5)