##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632286.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	187482
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.64069083965394	34.0	31.0	34.0	31.0	34.0
2	32.819086632316704	34.0	31.0	34.0	31.0	34.0
3	32.902006592632894	34.0	31.0	34.0	31.0	34.0
4	36.3286822201598	37.0	37.0	37.0	35.0	37.0
5	36.18379364418984	37.0	35.0	37.0	35.0	37.0
6	36.20075527250616	37.0	36.0	37.0	35.0	37.0
7	36.18002261550442	37.0	36.0	37.0	35.0	37.0
8	36.162138231936936	37.0	36.0	37.0	35.0	37.0
9	37.95376622822457	39.0	38.0	39.0	35.0	39.0
10	37.90452416765343	39.0	38.0	39.0	35.0	39.0
11	37.974109514513394	39.0	38.0	39.0	35.0	39.0
12	37.9362125430708	39.0	38.0	39.0	35.0	39.0
13	37.95889738748253	39.0	38.0	39.0	35.0	39.0
14	39.32263363949606	40.0	39.0	41.0	36.0	41.0
15	39.33703502202878	40.0	39.0	41.0	36.0	41.0
16	39.337621745020854	40.0	39.0	41.0	36.0	41.0
17	39.32094814435519	40.0	39.0	41.0	36.0	41.0
18	39.28619280784289	40.0	39.0	41.0	36.0	41.0
19	39.30824825849948	40.0	39.0	41.0	36.0	41.0
20	39.28470999882656	40.0	39.0	41.0	36.0	41.0
21	39.27335424200723	40.0	39.0	41.0	36.0	41.0
22	39.211204275610456	40.0	39.0	41.0	36.0	41.0
23	39.16913090323338	40.0	39.0	41.0	36.0	41.0
24	39.12274244994186	40.0	39.0	41.0	36.0	41.0
25	39.058874985331926	40.0	39.0	41.0	36.0	41.0
26	38.968007595395825	40.0	38.0	41.0	35.0	41.0
27	38.884724933593624	40.0	38.0	41.0	35.0	41.0
28	38.807496186300554	40.0	38.0	41.0	35.0	41.0
29	38.75055738684247	40.0	38.0	41.0	35.0	41.0
30	38.679297212532404	40.0	38.0	41.0	35.0	41.0
31	38.612277445301416	40.0	38.0	41.0	35.0	41.0
32	38.532333770708654	40.0	38.0	41.0	35.0	41.0
33	38.45568641256227	40.0	38.0	41.0	34.0	41.0
34	38.370904940207595	40.0	38.0	41.0	34.0	41.0
35	38.33372803789164	40.0	38.0	41.0	34.0	41.0
36	38.297217866248495	40.0	38.0	41.0	34.0	41.0
37	38.23451317993194	40.0	38.0	41.0	34.0	41.0
38	38.19951782037742	40.0	38.0	41.0	34.0	41.0
39	38.1461847003979	40.0	38.0	41.0	34.0	41.0
40	38.06129655113558	40.0	37.0	41.0	33.0	41.0
41	38.01095038457025	40.0	37.0	41.0	33.0	41.0
42	37.95797463223136	40.0	37.0	41.0	33.0	41.0
43	37.06314206163792	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.0
16	1.0
17	2.0
18	2.0
19	9.0
20	10.0
21	18.0
22	51.0
23	71.0
24	132.0
25	191.0
26	323.0
27	522.0
28	658.0
29	1023.0
30	1390.0
31	1902.0
32	2518.0
33	3602.0
34	5236.0
35	8066.0
36	11967.0
37	21499.0
38	51317.0
39	76969.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.29695650782475	17.412871635677025	11.816601060368463	27.47357079612976
2	20.633447477624518	19.282917826778036	32.28523271567404	27.798401979923405
3	21.410588749853318	20.67078439530195	28.363789590467352	29.554837264377383
4	16.35890378809699	14.99823983102378	31.931598766814943	36.71125761406429
5	17.54835130839227	33.430409319294654	32.045743058000234	16.975496314312842
6	37.14009878281648	32.023340907393774	14.722480024749043	16.114080285040696
7	31.335274853052557	27.657588461825668	19.80776821241506	21.199368472706716
8	28.884372899798382	30.78962247042383	19.06209662794295	21.263908001834846
9	27.509307560192447	13.434356364877695	18.571382852753864	40.484953222175996
10	18.73993236684055	24.735707961297617	31.446218837008356	25.07814083485348
11	38.182332170555036	19.976317726501744	19.745895605978173	22.095454496965043
12	23.867891317566485	23.477987220106463	26.80310643155076	25.851015030776285
13	32.6810040430548	17.959590787382254	22.163727717860915	27.195677451702032
14	24.561291217290194	19.512273178225108	22.588301810307122	33.33813379417758
15	27.686391226891118	25.314430185297788	19.920845734523848	27.07833285328725
16	27.293286822201594	23.786283483214387	21.62500933422942	27.295420360354594
17	26.061701923384646	24.461548308637628	23.01340928729158	26.46334048068615
18	26.006763315944998	22.882730075420575	24.163386351756436	26.947120256877994
19	26.774837051023564	23.39957969298386	23.739345643848473	26.086237612144096
20	26.61002122870462	23.20116064475523	23.745212873769216	26.443605252770936
21	27.816003669685625	22.84592654228139	22.946202835472203	26.39186695256078
22	27.887477197810988	22.920066993098004	22.806456086450964	26.38599972264004
23	27.09060069766698	22.77711993684727	22.99794113568236	27.134338229803394
24	26.940719642419005	23.197960337525735	22.794721626609487	27.066598393445773
25	26.801506277936017	22.818190546292445	23.063014049348737	27.317289126422807
26	26.996191634396904	23.22196264174694	23.181425416840018	26.600420307016144
27	27.174875454710318	22.94513606639571	22.912599609562516	26.967388869331455
28	26.41586925678198	22.964337909772674	23.751080103689954	26.86871272975539
29	26.281456353143234	22.973405446922904	24.024706371811693	26.720431828122166
30	25.948624401275854	23.50145613978942	23.764414717146177	26.785504741788547
31	26.693762601209713	23.12542004032387	23.100350967026166	27.080466391440243
32	26.059568385231646	23.172357879689784	23.720677185009762	27.047396550068807
33	26.238252205545066	22.8677953083496	23.729211337621745	27.16474114848359
34	26.726832442581156	22.771252706926532	23.669472269337856	26.83244258115446
35	26.468674326068637	22.759518247085055	24.264196029485497	26.507611397360815
36	26.152377294886975	22.86939546196435	23.642803042425406	27.335424200723267
37	25.58325599257529	22.05811757928761	24.993866077810136	27.364760350326968
38	25.663263673312635	21.925838213801857	25.61365891125548	26.797239201630024
39	25.05040483886453	21.743420701720698	26.21958374670635	26.98659071270842
40	24.97786454166267	21.424990132386043	27.0767326996725	26.520412626278787
41	24.04337483065041	21.451125974760245	27.462369720826533	27.043129473762818
42	23.9660340726043	21.724218858343736	27.16474114848359	27.145005920568376
43	23.18569249314601	21.03455265038777	28.058160250050673	27.72159460641555
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5.0
1	4.5
2	4.0
3	10.0
4	16.0
5	16.0
6	23.0
7	30.0
8	32.5
9	35.0
10	46.5
11	58.0
12	58.0
13	105.0
14	152.0
15	214.0
16	276.0
17	313.0
18	350.0
19	350.0
20	451.5
21	553.0
22	585.5
23	618.0
24	844.5
25	1071.0
26	1071.0
27	1306.0
28	1541.0
29	1913.5
30	2286.0
31	2781.5
32	3277.0
33	3277.0
34	3925.5
35	4574.0
36	5335.5
37	6097.0
38	6740.0
39	7383.0
40	7383.0
41	7990.5
42	8598.0
43	9265.5
44	9933.0
45	10580.0
46	11227.0
47	11227.0
48	11556.5
49	11886.0
50	12536.0
51	13186.0
52	13966.0
53	14746.0
54	14746.0
55	14808.0
56	14870.0
57	14696.0
58	14522.0
59	14069.0
60	13616.0
61	13616.0
62	12956.0
63	12296.0
64	10774.5
65	9253.0
66	8323.5
67	7394.0
68	7394.0
69	6360.5
70	5327.0
71	4710.5
72	4094.0
73	3671.0
74	3248.0
75	3248.0
76	2860.0
77	2472.0
78	1795.5
79	1119.0
80	889.0
81	659.0
82	659.0
83	491.5
84	324.0
85	266.5
86	209.0
87	157.0
88	105.0
89	105.0
90	82.0
91	59.0
92	33.0
93	7.0
94	5.0
95	3.0
96	3.0
97	3.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	187482.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.11770476883301
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.77874379207515	63.304015297759086
2	7.017442468424148	10.121636883401669
3	1.9563792957348565	4.232687533969914
4	0.9450889440666554	2.7263058179394872
5	0.5306586030699597	1.9134940234620343
6	0.32941871607099316	1.4254153026561545
7	0.2546994805460795	1.28578393599581
8	0.18921187548882637	1.0916420940208296
9	0.14659322036794004	0.9514769928846818
>10	0.8052139701996756	10.437609480861878
>50	0.03961670974954874	1.900663274668693
>100	0.00693292420617103	0.609269362379705
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0016001536147470157	0.0
18	0.0	0.0	0.0	0.0016001536147470157	0.0
19	0.0	0.0	0.0	0.0016001536147470157	0.0
20	0.0	0.0	0.0	0.002133538152996021	0.0
21	0.0	0.0	0.0	0.002133538152996021	0.0
22	0.0	0.0	0.0	0.0037336917677430366	0.0
23	0.0	0.0	0.0	0.005333845382490052	0.0
24	0.0	0.0	0.0	0.006400614458988063	0.0
25	0.0	0.0	0.0	0.009067537150233089	0.0
26	0.0	0.0	0.0	0.01440138253272314	0.0
27	0.0	0.0	0.0	0.05013814659540649	0.0
28	5.333845382490053E-4	0.0	0.0	0.11467767572353613	0.0
29	5.333845382490053E-4	0.0	0.0	0.203219509072871	0.0
30	5.333845382490053E-4	0.0	0.0	0.3168304157199091	0.0
31	5.333845382490053E-4	0.0	0.0	0.5979240673771349	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCAC	25	0.0054847603	29.6	23
GGTATCA	100	0.0	29.6	1
CGCACGG	25	0.0054847603	29.6	25
CAGAGCT	40	0.0019251964	23.125	4
GTATCAA	145	7.2759576E-12	19.13793	2
TGCATCA	50	0.007013473	18.5	14
TTTATTC	75	2.0556622E-4	17.266666	3
GCTTTAT	70	0.0025816564	15.857143	1
CTTATAC	160	1.0766598E-8	15.03125	37
TCTCTTA	560	0.0	14.205358	37
CCAGGAC	95	0.0012382838	13.631579	3
TCTTATA	245	7.2759576E-12	13.591836	37
TAGAGCT	85	0.009366385	13.058825	4
CCGGCGT	85	0.009366385	13.058825	26
GGGCATC	85	0.009366385	13.058825	32
GCTGCGT	115	3.5523516E-4	12.869565	35
CGGGTGT	165	3.7552072E-6	12.333333	14
GTCTAGT	105	0.002629361	12.333333	32
GTCTCTT	810	0.0	11.876543	37
TGCGTCT	125	7.169264E-4	11.840001	37
>>END_MODULE