FastQCFastQC Report
Fri 10 Feb 2017
ERR1632285.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632285.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11232
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2952.626424501424501No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2592.305911680911681No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1851.6470797720797723No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1080.9615384615384616No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT630.5608974358974359No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT570.5074786324786325RNA PCR Primer, Index 39 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT340.30270655270655267No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.2581908831908832No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.2136752136752137No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210.18696581196581197No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA200.17806267806267806No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.17806267806267806No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.15135327635327633No Hit
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC170.15135327635327633RNA PCR Primer, Index 39 (95% over 21bp)
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA150.13354700854700854No Hit
GTTCCGACCTGCACGAATGGCGTAACGATGGCCACACTGTCTC150.13354700854700854No Hit
GGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCG140.12464387464387464No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTG140.12464387464387464No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.12464387464387464No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG130.11574074074074073No Hit
GTCTCAGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGC120.10683760683760685No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA120.10683760683760685No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA120.10683760683760685No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.10683760683760685No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC120.10683760683760685No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA120.10683760683760685No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.10683760683760685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2001.8189894E-1215.7251
GTATCAA2508.731149E-1112.582
AACGCAG2601.70985E-1012.0961547
AGTACAT1755.6961508E-611.62857157
GAGTACA1755.6961508E-611.62857156
ATCAACG2754.3473847E-1011.4363644
CAACGCA2754.3473847E-1011.4363646
TCAACGC2805.8935257E-1011.2321425
TATCAAC2951.4024408E-910.6610163
ATGGGAA1250.00872936310.3612
CATGGGA1250.00872936310.3611
CTGTCTC1250.00872936310.3637
ACATGGG2002.5135396E-510.17510
GTACATG2053.3016724E-59.9268288
TACATGG2053.3016724E-59.9268289
AGTACTT1707.126379E-49.79411713
GAGTACT1707.126379E-49.79411712
CGCAGAG3103.700916E-89.5483889
ACGCAGA3351.1452357E-89.388068
CAGAGTA3206.0557795E-89.2511
Produced by FastQC (version 0.11.5)