FastQCFastQC Report
Fri 10 Feb 2017
ERR1632284.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632284.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1627445
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT29210.17948379207899498No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA25940.1593909471595046No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT24060.14783909748102086No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC22490.13819207407930836No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT20680.12707034646332133No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG19580.12031128548122978No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT18620.11441246862413168No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG17420.10703894755275907No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC16290.10009554854388321No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8400.025.7678571
TGTGCGT555.144358E-420.18181810
CTTATAC11300.019.31858337
GTATTAT902.153336E-618.51
TAACGGT609.2390657E-418.54
TTTACGC752.067932E-417.26666633
ACACTAT1005.880811E-616.6500026
TCTTATA20250.016.35308537
TAACCCG1708.54925E-1116.3235328
GTATCAA14000.015.5928572
ATCTCGC7650.014.993463511
GTATAGA1752.239176E-914.81
ATAACGA1508.106872E-814.812
TAGTACA1001.0938072E-414.84
CTAGCAC3050.014.5573773
TATTAGA908.2766206E-414.3888882
ATACCAT908.2766206E-414.3888886
CAAGACG3000.014.1833334
CGGCCTT7850.014.14012724
ATTTACG1051.6562993E-414.09523832
Produced by FastQC (version 0.11.5)