##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632284.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1627445
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.524401746295574	31.0	31.0	34.0	30.0	34.0
2	31.71982278971025	31.0	31.0	34.0	30.0	34.0
3	31.80834928369315	31.0	31.0	34.0	30.0	34.0
4	35.59887861033706	37.0	35.0	37.0	33.0	37.0
5	34.83771433135989	37.0	35.0	37.0	32.0	37.0
6	34.79395432718157	36.0	35.0	37.0	32.0	37.0
7	34.77931420109435	35.0	35.0	37.0	32.0	37.0
8	34.71843656774883	35.0	35.0	37.0	32.0	37.0
9	36.338374568725825	38.0	35.0	39.0	32.0	39.0
10	36.090556670117884	37.0	35.0	39.0	32.0	39.0
11	36.23757546337971	37.0	35.0	39.0	32.0	39.0
12	36.16876023460086	37.0	35.0	39.0	32.0	39.0
13	36.248222213346686	37.0	35.0	39.0	32.0	39.0
14	37.27022049900304	39.0	36.0	40.0	32.0	41.0
15	37.221241885286446	38.0	36.0	40.0	32.0	41.0
16	37.1908107493648	38.0	36.0	40.0	32.0	41.0
17	37.16087425381503	38.0	36.0	40.0	32.0	41.0
18	37.13662089963102	38.0	36.0	40.0	32.0	41.0
19	37.1801769030597	39.0	36.0	40.0	32.0	41.0
20	37.14154825508696	39.0	36.0	40.0	32.0	41.0
21	37.099353280756034	39.0	36.0	40.0	32.0	41.0
22	37.05903056631714	39.0	36.0	40.0	31.0	41.0
23	36.99103809959784	39.0	36.0	40.0	31.0	41.0
24	36.907258924264724	38.0	36.0	40.0	31.0	41.0
25	36.81559745490631	38.0	36.0	40.0	31.0	41.0
26	36.65289456786558	38.0	36.0	40.0	31.0	41.0
27	36.54127482034723	38.0	35.0	40.0	30.0	41.0
28	36.43606696386053	38.0	35.0	40.0	30.0	41.0
29	36.33369791298631	38.0	35.0	40.0	30.0	41.0
30	36.232302166893504	38.0	35.0	40.0	30.0	41.0
31	36.11145814451487	38.0	35.0	40.0	30.0	41.0
32	35.982312151869955	38.0	35.0	40.0	29.0	41.0
33	35.880008233765196	38.0	34.0	40.0	29.0	41.0
34	35.79276350352854	38.0	34.0	40.0	29.0	41.0
35	35.74356675648025	38.0	34.0	40.0	29.0	41.0
36	35.68677282488809	38.0	34.0	40.0	29.0	41.0
37	35.62627861463828	38.0	34.0	40.0	28.0	41.0
38	35.54883882404628	38.0	34.0	40.0	28.0	41.0
39	35.49003745134244	38.0	34.0	40.0	28.0	41.0
40	35.40738396689289	38.0	34.0	40.0	27.0	41.0
41	35.35385527621517	38.0	34.0	40.0	27.0	41.0
42	35.29486403534374	38.0	34.0	40.0	27.0	41.0
43	34.30207165219101	37.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	2.0
13	2.0
14	10.0
15	24.0
16	59.0
17	110.0
18	182.0
19	394.0
20	682.0
21	1181.0
22	1841.0
23	3128.0
24	4874.0
25	7098.0
26	10262.0
27	14953.0
28	21004.0
29	28994.0
30	39279.0
31	52980.0
32	70416.0
33	95173.0
34	129573.0
35	177131.0
36	237527.0
37	301031.0
38	308550.0
39	120981.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.802825901950605	19.22602607154159	13.013281554829808	25.957866471678
2	19.051580852194697	21.424072702917762	34.246441508007955	25.277904936879587
3	20.38815443839884	22.59375892887317	30.18172657140487	26.836360061323116
4	15.891719843066893	16.100144705351024	33.345765909139786	34.66236954244229
5	15.523719695596473	35.8775258150045	33.21992448285503	15.378830006544
6	34.76467714730759	34.253569245043614	15.797338773353323	15.184414834295476
7	29.39638513129476	30.26179072103819	20.824236763761604	19.517587383905447
8	26.916178426920723	33.59419212323612	20.114596806650916	19.375032643192245
9	26.73485125457389	14.88037998211921	19.36544706579946	39.01932169750744
10	17.28304182322598	26.980819628313093	32.476489220833884	23.259649327627045
11	36.155569005404175	21.519314016756326	21.420078712337435	20.90503826550206
12	22.177339326367402	25.180820242773184	28.07720076561727	24.564639665242144
13	30.488157817929327	19.284399780023286	24.656808678634302	25.570633723413078
14	23.451668105527375	20.52272119795139	24.091443950486806	31.934166746034425
15	25.92800371133894	27.394535606426025	21.832381432245022	24.845079249990015
16	25.806463505679144	25.967267711044002	23.1003812724854	25.125887510791458
17	24.67567260337523	26.14533824491765	24.35725938510979	24.821729766597336
18	23.91398787670244	24.947448300864238	26.06060419860579	25.077959623827535
19	25.42912356485165	25.032735361256446	25.932796500035334	23.605344573856566
20	25.49185993996725	24.404449919966574	25.712266773992365	24.391423366073813
21	25.440982644574778	25.454562212547888	24.41526441753792	24.68919072533941
22	25.5101094046189	24.759239175517454	24.54270344005481	25.18794797980884
23	24.695150988205437	25.25338797931727	24.869964883605896	25.18149614887139
24	25.816540651143356	25.042935398738514	24.646301411107597	24.494222539010536
25	25.485285217011942	24.259744568940885	25.150896036425195	25.10407417762198
26	25.241160223540582	24.739699344678314	25.01510035669408	25.004040075087026
27	25.36767755592355	24.322665282083268	24.83580090264187	25.47385625935131
28	24.44568019195733	25.002442478854892	25.47680566777986	25.07507166140791
29	24.68120274417876	25.42107413768207	25.173815397755376	24.723907720383792
30	24.199711818218127	26.20721437590825	24.92667954984654	24.666394256027086
31	25.1614033039519	25.004347305131663	25.155381595076946	24.67886779583949
32	24.00111831736249	25.003671399033458	25.36073415691467	25.634476126689382
33	24.401746295573737	24.549769731081543	25.598591657475367	25.449892315869356
34	24.864127512757726	24.429458445600314	25.5586517516721	25.14776228996986
35	24.928031362042958	24.380055854422118	25.62808574176086	25.06382704177407
36	24.335077375886744	24.660864115223553	25.847140763589554	25.15691774530015
37	25.092891003997064	23.252337252564605	25.977774978570707	25.67699676486763
38	23.978076064014452	23.915216796881	27.079133242598058	25.02757389650649
39	23.987845979434024	23.176021309475896	27.466120206827267	25.370012504262817
40	23.620767522097523	23.10161019266396	28.182765008955755	25.094857276282763
41	22.73195100295248	23.55231666815161	28.661552310523554	25.054180018372357
42	23.488965832946736	22.689614702801016	28.340619805892057	25.480799658360194
43	22.25371671546504	22.71277984816691	28.89701341673605	26.136490019631996
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	74.0
1	82.0
2	90.0
3	211.5
4	333.0
5	333.0
6	456.0
7	579.0
8	625.0
9	671.0
10	995.0
11	1319.0
12	1319.0
13	2153.5
14	2988.0
15	5157.5
16	7327.0
17	7204.0
18	7081.0
19	7081.0
20	7973.0
21	8865.0
22	9784.0
23	10703.0
24	12547.0
25	14391.0
26	14391.0
27	16807.0
28	19223.0
29	26260.5
30	33298.0
31	36630.5
32	39963.0
33	39963.0
34	45635.5
35	51308.0
36	56198.0
37	61088.0
38	73022.5
39	84957.0
40	84957.0
41	94471.5
42	103986.0
43	101018.0
44	98050.0
45	105460.0
46	112870.0
47	112870.0
48	117343.0
49	121816.0
50	128482.5
51	135149.0
52	142645.0
53	150141.0
54	150141.0
55	132898.0
56	115655.0
57	108000.0
58	100345.0
59	91593.5
60	82842.0
61	82842.0
62	76670.0
63	70498.0
64	63410.5
65	56323.0
66	49356.5
67	42390.0
68	42390.0
69	36628.5
70	30867.0
71	27332.5
72	23798.0
73	19264.5
74	14731.0
75	14731.0
76	12021.0
77	9311.0
78	7735.0
79	6159.0
80	4869.0
81	3579.0
82	3579.0
83	2814.0
84	2049.0
85	1689.0
86	1329.0
87	1052.0
88	775.0
89	775.0
90	596.5
91	418.0
92	249.0
93	80.0
94	50.0
95	20.0
96	20.0
97	12.5
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1627445.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.658891544961044
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.20414199129377	47.664817393735085
2	7.092183252193818	7.753017503973019
3	2.0938005865933316	3.433344575381421
4	0.9411952789936074	2.0577876270856374
5	0.5482082736751006	1.4982228287428825
6	0.35843890470035444	1.1755123930506781
7	0.24706377181324463	0.9452962335760398
8	0.18425131634430908	0.8056778173663917
9	0.14082603790675344	0.6927655619386626
>10	0.9472730028935417	10.201259162659532
>50	0.11482248852870344	4.413955187085195
>100	0.1143727852620044	12.803101214648136
>500	0.009925741701889753	3.65893355632985
>1k	0.003496568099529345	2.8963089444273704
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2921	0.17948379207899498	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	2594	0.1593909471595046	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2406	0.14783909748102086	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	2249	0.13819207407930836	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2068	0.12707034646332133	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	1958	0.12031128548122978	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	1862	0.11441246862413168	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	1742	0.10703894755275907	No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC	1629	0.10009554854388321	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	6.14460089281051E-5	0.0	0.0
11	0.0	0.0	6.14460089281051E-5	0.0	0.0
12	0.0	0.0	6.14460089281051E-5	0.0	0.0
13	0.0	0.0	6.14460089281051E-5	0.0	0.0
14	0.0	0.0	6.14460089281051E-5	6.14460089281051E-5	0.0
15	0.0	0.0	6.14460089281051E-5	1.228920178562102E-4	0.0
16	0.0	0.0	6.14460089281051E-5	1.8433802678431528E-4	0.0
17	0.0	0.0	6.14460089281051E-5	3.072300446405255E-4	0.0
18	0.0	6.14460089281051E-5	6.14460089281051E-5	3.072300446405255E-4	0.0
19	0.0	6.14460089281051E-5	6.14460089281051E-5	4.3012206249673566E-4	0.0
20	0.0	6.14460089281051E-5	6.14460089281051E-5	4.3012206249673566E-4	0.0
21	0.0	6.14460089281051E-5	6.14460089281051E-5	7.987981160653663E-4	0.0
22	0.0	6.14460089281051E-5	6.14460089281051E-5	9.216901339215765E-4	0.0
23	0.0	6.14460089281051E-5	6.14460089281051E-5	0.001228920178562102	0.0
24	0.0	6.14460089281051E-5	6.14460089281051E-5	0.002457840357124204	0.0
25	0.0	6.14460089281051E-5	6.14460089281051E-5	0.003748206544614411	0.0
26	0.0	6.14460089281051E-5	6.14460089281051E-5	0.005960262866026194	0.0
27	0.0	6.14460089281051E-5	6.14460089281051E-5	0.018741032723072055	0.0
28	6.14460089281051E-5	6.14460089281051E-5	6.14460089281051E-5	0.06482553941915088	0.0
29	6.14460089281051E-5	6.14460089281051E-5	6.14460089281051E-5	0.13198602717756974	0.0
30	6.14460089281051E-5	6.14460089281051E-5	6.14460089281051E-5	0.21862489976619795	0.0
31	6.14460089281051E-5	6.14460089281051E-5	6.14460089281051E-5	0.553075526361874	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	840	0.0	25.767857	1
TGTGCGT	55	5.144358E-4	20.181818	10
CTTATAC	1130	0.0	19.318583	37
GTATTAT	90	2.153336E-6	18.5	1
TAACGGT	60	9.2390657E-4	18.5	4
TTTACGC	75	2.067932E-4	17.266666	33
ACACTAT	100	5.880811E-6	16.650002	6
TCTTATA	2025	0.0	16.353085	37
TAACCCG	170	8.54925E-11	16.32353	28
GTATCAA	1400	0.0	15.592857	2
ATCTCGC	765	0.0	14.9934635	11
GTATAGA	175	2.239176E-9	14.8	1
ATAACGA	150	8.106872E-8	14.8	12
TAGTACA	100	1.0938072E-4	14.8	4
CTAGCAC	305	0.0	14.557377	3
TATTAGA	90	8.2766206E-4	14.388888	2
ATACCAT	90	8.2766206E-4	14.388888	6
CAAGACG	300	0.0	14.183333	4
CGGCCTT	785	0.0	14.140127	24
ATTTACG	105	1.6562993E-4	14.095238	32
>>END_MODULE