FastQCFastQC Report
Fri 10 Feb 2017
ERR1632283.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632283.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170530
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACAGGAGCATCCAGGCACTGCAGCTCGTCTGGGTAGTCGGC2440.14308332844660765No Hit
CTCTACCACCATGAATCTACTTCTGATCCTTACCTTTGTTGCA2230.13076877968685863No Hit
GGGCAGAGAGATGGCGGACACGCGGGAATTGATGACGGCAGGT2130.1249047088488829No Hit
GTTGTAGACCTTGGTGTAGACTCCAGGCCTGTTCTTCTGGGCA1920.11259016008913388No Hit
CTCCTCACCTGCCGTCATCAATTCCCGCGTGTCCGCCATCTCT1770.1037940538321703No Hit
GGATTAAGGACACCATAGCTGCCAACAGCTAAAGCCCCTGGTC1770.1037940538321703No Hit
GGAATGAACAGTTCATCAATGCAGCCAAGATCATCCGCCACCC1760.10320764674837271No Hit
CTGCTACAAGTCCCGCATCCAGGTGAGACTGGGAGAGCACAAC1730.10144842549698No Hit
GGTATAGAGACTGCAGAGGGACCAGGGGCTTTAGCTGTTGGCA1730.10144842549698No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCACC250.00548338529.611
ACATGTA405.910956E-527.758
CTAGACA706.508344E-926.428574
GACATGT451.3167663E-424.6666667
TCTAGAC751.3564204E-824.6666643
TAGACAT701.9030085E-723.7857135
CTCTAGT701.9030085E-723.78571327
TTCTAGA802.6913767E-823.1252
AAGGTAC400.001924485323.12523
GTAGCAC400.001924485323.1253
GTACTGA400.001924485323.12526
CGGTCGG400.001924485323.12521
TCTAGAG652.6606613E-622.769233
TCTAGTT806.8888585E-720.812528
ACTGAGA450.00381265920.55555510
GCTCTAG450.00381265920.5555551
GACTGAG450.00381265920.5555559
ATCTACT759.190111E-619.73333215
GTTCTAG851.2326291E-619.5882361
CTAGAGA500.007010933518.54
Produced by FastQC (version 0.11.5)