FastQCFastQC Report
Fri 10 Feb 2017
ERR1632282.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632282.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences288250
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGA200.001839649637.06
TATATAA250.00548959829.62
TGTACCG509.06027E-625.9000025
GGTATCA1500.020.9666671
TTTCATC450.003818961620.55555517
GCTTTAT450.003818961620.5555551
TAACCCG555.1302573E-420.18181828
CGGGTAA609.2139485E-418.524
CTGACCG609.2139485E-418.59
GCGGGTA701.2151802E-418.523
GTCAAAT500.007022412518.513
TTCGGGC500.007022412518.59
GGTTGAT650.001576054517.0769231
GAACACT650.001576054517.07692325
GTATCAA1851.8189894E-1217.0000022
CGCGGGT803.3715813E-416.187522
CTATTGG803.3715813E-416.18751
ACCATCC700.0025861815.8571438
GTAACCC700.0025861815.85714327
TACCGCA700.0025861815.85714323
Produced by FastQC (version 0.11.5)