##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632282.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	288250
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.18063833477884	33.0	31.0	34.0	30.0	34.0
2	32.37270078057242	34.0	31.0	34.0	31.0	34.0
3	32.464728534258455	34.0	31.0	34.0	31.0	34.0
4	36.03677710320902	37.0	35.0	37.0	35.0	37.0
5	35.7625568083261	37.0	35.0	37.0	35.0	37.0
6	35.763379011274935	37.0	35.0	37.0	35.0	37.0
7	35.728763226366	37.0	35.0	37.0	33.0	37.0
8	35.701453599306156	37.0	35.0	37.0	33.0	37.0
9	37.430109280138765	39.0	37.0	39.0	35.0	39.0
10	37.2993130962706	39.0	37.0	39.0	34.0	39.0
11	37.40935646140503	39.0	37.0	39.0	35.0	39.0
12	37.326754553339114	39.0	37.0	39.0	34.0	39.0
13	37.39167736339983	39.0	37.0	39.0	35.0	39.0
14	38.61665568083261	40.0	38.0	41.0	35.0	41.0
15	38.593814397224634	40.0	38.0	41.0	35.0	41.0
16	38.583607979184734	40.0	38.0	41.0	35.0	41.0
17	38.553106678230705	40.0	38.0	41.0	35.0	41.0
18	38.53642671292281	40.0	38.0	41.0	35.0	41.0
19	38.54240763226366	40.0	38.0	41.0	34.0	41.0
20	38.4983972246314	40.0	38.0	41.0	34.0	41.0
21	38.480010407632264	40.0	38.0	41.0	34.0	41.0
22	38.42718820468343	40.0	38.0	41.0	34.0	41.0
23	38.38973807458803	40.0	38.0	41.0	34.0	41.0
24	38.3136409366869	40.0	38.0	41.0	34.0	41.0
25	38.241113616652214	40.0	38.0	41.0	34.0	41.0
26	38.10484301821336	40.0	38.0	41.0	34.0	41.0
27	38.00795490026019	40.0	38.0	41.0	33.0	41.0
28	37.92326799653079	40.0	37.0	41.0	33.0	41.0
29	37.84875628794449	40.0	37.0	41.0	33.0	41.0
30	37.753880312228965	40.0	37.0	41.0	33.0	41.0
31	37.681665221162184	40.0	37.0	41.0	33.0	41.0
32	37.57529921942758	39.0	37.0	41.0	33.0	41.0
33	37.48896097137901	39.0	37.0	41.0	32.0	41.0
34	37.40216131830009	39.0	37.0	40.0	32.0	41.0
35	37.364659150043366	39.0	36.0	40.0	32.0	41.0
36	37.330990459670424	39.0	36.0	40.0	32.0	41.0
37	37.2799236773634	39.0	36.0	40.0	32.0	41.0
38	37.22581092801388	39.0	36.0	40.0	32.0	41.0
39	37.15901821335646	39.0	36.0	40.0	31.0	41.0
40	37.0876565481353	39.0	36.0	40.0	31.0	41.0
41	37.041474414570686	39.0	36.0	40.0	31.0	41.0
42	36.99384908933218	39.0	35.0	40.0	31.0	41.0
43	36.02104423243712	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	3.0
17	2.0
18	9.0
19	25.0
20	49.0
21	77.0
22	156.0
23	257.0
24	377.0
25	583.0
26	897.0
27	1352.0
28	1838.0
29	2557.0
30	3569.0
31	4808.0
32	6349.0
33	8686.0
34	12236.0
35	17962.0
36	28397.0
37	48424.0
38	88148.0
39	61489.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.226366001734604	17.3918473547268	11.723156981786644	27.65862966175195
2	21.612836079791848	19.210754553339115	31.345359930615786	27.831049436253252
3	22.104423243712056	20.05481352992194	27.213876843018213	30.62688638334779
4	17.046313963573287	14.583868169991327	30.895403295750217	37.47441457068517
5	18.377450130095404	32.79722463139636	31.29505637467476	17.530268863833477
6	38.60121422376409	31.081352992194276	14.072506504770166	16.244926279271464
7	32.299739809193404	27.041803989592367	19.175715524718125	21.4827406764961
8	28.825672159583693	30.63555941023417	18.40381613183001	22.134952298352125
9	27.844926279271466	13.161838681699914	17.902862098872507	41.090372940156115
10	19.207285342584562	24.296964440589765	30.598438855160452	25.89731136166522
11	39.285689505637464	19.225672159583695	19.310320901994796	22.17831743278404
12	24.116218560277535	22.66921075455334	26.65151777970512	26.56305290546401
13	33.16912402428447	17.22983521248916	21.526799653078925	28.07424111014744
14	24.84614050303556	18.713616652211623	22.06175195143105	34.37849089332177
15	28.25117085862966	24.88326105810928	19.630182133564613	27.235385949696443
16	28.193235039028618	23.240589765828272	21.398785776235908	27.167389418907202
17	26.705290546400697	23.579531656548134	22.577276669557676	27.137901127493496
18	27.048395490026017	22.31673894189072	23.372419774501303	27.262445793581957
19	27.541023417172596	22.814570685169127	22.932523850823937	26.711882046834347
20	27.767215958369473	22.337901127493495	22.71153512575889	27.183347788378143
21	28.04301821335646	22.453078924544666	22.28551604509974	27.218386816999136
22	28.004509973980916	22.541890719861232	22.17033824804857	27.283261058109282
23	27.741890719861235	22.177970511708587	22.305984388551607	27.77415437987858
24	27.738421509106676	22.910320901994798	22.088117953165654	27.26313963573287
25	27.83694709453599	22.018733738074587	22.153686036426713	27.990633130962706
26	27.46296617519514	22.694535993061578	22.68482220294883	27.157675628794447
27	27.616652211621854	22.25568083261058	22.315351257588897	27.812315698178665
28	26.952298352124892	22.502688638334778	22.991500433651343	27.553512575888984
29	26.861405030355595	22.83052905464007	23.14518647007806	27.16287944492628
30	26.61543798785776	22.99045967042498	22.920381613183	27.473720728534257
31	27.424111014744145	22.53599306157849	22.350043365134432	27.68985255854293
32	26.781613183000868	22.64562012142238	22.76287944492628	27.809887250650476
33	26.568950563746746	22.317085862966174	23.44457935819601	27.669384215091064
34	27.16634865568083	21.961144839549	23.117779705117087	27.754726799653078
35	26.89679098005204	21.98751084128361	23.915004336513444	27.20069384215091
36	26.37502168256722	21.9316565481353	23.809887250650476	27.88343451864701
37	26.185949696444062	20.901994796183867	24.92072853425846	27.991326973113615
38	25.48308759757155	20.871812662619256	25.77762359063313	27.867476149176063
39	25.11882046834345	20.49262792714657	26.59392888117953	27.79462272333044
40	24.73894189071986	20.611621856027753	26.928360797918472	27.72107545533391
41	24.07875108412836	20.65498699045967	27.8959236773634	27.37033824804857
42	24.07007805724198	20.970685169124025	27.738421509106676	27.22081526452732
43	23.210754553339115	20.512055507372075	28.278577623590635	27.99861231569818
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4.0
1	3.5
2	3.0
3	10.0
4	17.0
5	17.0
6	20.5
7	24.0
8	26.0
9	28.0
10	37.5
11	47.0
12	47.0
13	102.0
14	157.0
15	214.5
16	272.0
17	300.0
18	328.0
19	328.0
20	416.5
21	505.0
22	570.5
23	636.0
24	813.5
25	991.0
26	991.0
27	1324.5
28	1658.0
29	2029.5
30	2401.0
31	3055.0
32	3709.0
33	3709.0
34	4436.5
35	5164.0
36	6249.0
37	7334.0
38	8425.0
39	9516.0
40	9516.0
41	10596.0
42	11676.0
43	12885.5
44	14095.0
45	15209.0
46	16323.0
47	16323.0
48	17760.5
49	19198.0
50	20340.0
51	21482.0
52	22556.5
53	23631.0
54	23631.0
55	23742.0
56	23853.0
57	24508.5
58	25164.0
59	24636.5
60	24109.0
61	24109.0
62	22490.0
63	20871.0
64	18587.0
65	16303.0
66	14469.5
67	12636.0
68	12636.0
69	11026.0
70	9416.0
71	8098.5
72	6781.0
73	5398.5
74	4016.0
75	4016.0
76	3202.0
77	2388.0
78	1907.0
79	1426.0
80	1202.0
81	978.0
82	978.0
83	754.0
84	530.0
85	419.0
86	308.0
87	237.0
88	166.0
89	166.0
90	124.0
91	82.0
92	50.5
93	19.0
94	11.5
95	4.0
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	288250.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.86291044612284
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.04743490177853	72.71313139909937
2	5.507150780734562	8.796341792301995
3	1.450753819559594	3.4758426711257546
4	0.6753280405466686	2.1573465129573686
5	0.33631636341589277	1.3429601806524574
6	0.20334049152261965	0.9743618078724944
7	0.13619016148559338	0.7613579869256867
8	0.08657107317488671	0.5531054291352578
9	0.06802371174638111	0.48893144394726984
>10	0.45280386360206837	6.728598553912011
>50	0.032608547379417416	1.6587103847191358
>100	0.0034782450538029903	0.34931183735123394
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	6.938421509106678E-4	0.0
17	0.0	0.0	0.0	0.0017346053772766695	0.0
18	0.0	0.0	0.0	0.0020815264527320036	0.0
19	0.0	0.0	0.0	0.0024284475281873374	0.0
20	0.0	0.0	0.0	0.0024284475281873374	0.0
21	0.0	0.0	0.0	0.0027753686036426713	0.0
22	0.0	0.0	0.0	0.003122289679098005	0.0
23	0.0	0.0	0.0	0.004856895056374675	0.0
24	0.0	0.0	0.0	0.006938421509106678	0.0
25	0.0	0.0	0.0	0.010060711188204683	0.0
26	0.0	0.0	0.0	0.014917606244579358	0.0
27	0.0	0.0	0.0	0.044058976582827404	0.0
28	0.0	0.0	0.0	0.13321769297484823	0.0
29	0.0	0.0	0.0	0.23243712055507373	0.0
30	0.0	0.0	0.0	0.37606244579358195	0.0
31	0.0	0.0	0.0	0.7302688638334779	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACCGA	20	0.0018396496	37.0	6
TATATAA	25	0.005489598	29.6	2
TGTACCG	50	9.06027E-6	25.900002	5
GGTATCA	150	0.0	20.966667	1
TTTCATC	45	0.0038189616	20.555555	17
GCTTTAT	45	0.0038189616	20.555555	1
TAACCCG	55	5.1302573E-4	20.181818	28
CGGGTAA	60	9.2139485E-4	18.5	24
CTGACCG	60	9.2139485E-4	18.5	9
GCGGGTA	70	1.2151802E-4	18.5	23
GTCAAAT	50	0.0070224125	18.5	13
TTCGGGC	50	0.0070224125	18.5	9
GGTTGAT	65	0.0015760545	17.076923	1
GAACACT	65	0.0015760545	17.076923	25
GTATCAA	185	1.8189894E-12	17.000002	2
CGCGGGT	80	3.3715813E-4	16.1875	22
CTATTGG	80	3.3715813E-4	16.1875	1
ACCATCC	70	0.00258618	15.857143	8
GTAACCC	70	0.00258618	15.857143	27
TACCGCA	70	0.00258618	15.857143	23
>>END_MODULE