FastQCFastQC Report
Fri 10 Feb 2017
ERR1632281.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632281.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196211
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14870.7578576124682103No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11380.5799878702009572No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9230.4704119544775777No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4890.24922150134294202No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG200.001838218537.05
GATCGGA250.005485377729.616
ACACCGC250.005485377729.66
CCTTATT358.8430947E-426.4285727
GCCTTAT358.8430947E-426.4285726
CTTATTG400.001925515123.12528
TATTCCA400.001925515123.1255
AGATCGG450.0038146820.55555315
CTACAAG450.0038146820.5555531
TTTGAAC450.0038146820.5555533
ACAAGGT450.0038146820.5555533
TCTACAC656.8649184E-519.9230753
ATCTCGT500.00701461418.537
ATCTCCG500.00701461418.510
GCGCGGC500.00701461418.517
GGTATCA4950.017.5656571
CTTATAC2100.016.73809437
CAGGAGT904.4158027E-516.4444434
CTGAGCT803.3647157E-416.18754
CTACACC803.3647157E-416.18754
Produced by FastQC (version 0.11.5)