##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632281.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	196211
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.19574335791571	33.0	31.0	34.0	30.0	34.0
2	32.37699211563062	34.0	31.0	34.0	31.0	34.0
3	32.44819097807972	34.0	31.0	34.0	31.0	34.0
4	36.0453440428926	37.0	35.0	37.0	35.0	37.0
5	35.77147560534323	37.0	35.0	37.0	35.0	37.0
6	35.77938545749219	37.0	35.0	37.0	35.0	37.0
7	35.7616086763739	37.0	35.0	37.0	35.0	37.0
8	35.73290997956282	37.0	35.0	37.0	35.0	37.0
9	37.4356075857113	39.0	37.0	39.0	35.0	39.0
10	37.314039477908985	39.0	37.0	39.0	34.0	39.0
11	37.40587938494784	39.0	37.0	39.0	35.0	39.0
12	37.347462680481726	39.0	37.0	39.0	34.0	39.0
13	37.402694038560526	39.0	37.0	39.0	35.0	39.0
14	38.610954533639806	40.0	38.0	41.0	35.0	41.0
15	38.587979267217435	40.0	38.0	41.0	35.0	41.0
16	38.57284250118495	40.0	38.0	41.0	35.0	41.0
17	38.56089108153977	40.0	38.0	41.0	35.0	41.0
18	38.58382557552839	40.0	38.0	41.0	35.0	41.0
19	38.612350989496	40.0	38.0	41.0	35.0	41.0
20	38.603722523202066	40.0	38.0	41.0	35.0	41.0
21	38.576175647644625	40.0	38.0	41.0	35.0	41.0
22	38.51563877662313	40.0	38.0	41.0	34.0	41.0
23	38.45974486649576	40.0	38.0	41.0	34.0	41.0
24	38.38327107042928	40.0	38.0	41.0	34.0	41.0
25	38.312571670293714	40.0	38.0	41.0	34.0	41.0
26	38.18088180581109	40.0	38.0	41.0	34.0	41.0
27	38.0632533344206	40.0	38.0	41.0	34.0	41.0
28	37.969344226368555	40.0	37.0	41.0	33.0	41.0
29	37.863096360550635	40.0	37.0	41.0	33.0	41.0
30	37.740957438675714	40.0	37.0	41.0	33.0	41.0
31	37.61087808532651	39.0	37.0	41.0	33.0	41.0
32	37.483693574774094	39.0	36.0	41.0	33.0	41.0
33	37.363730881550985	39.0	36.0	40.0	32.0	41.0
34	37.26219223183206	39.0	36.0	40.0	32.0	41.0
35	37.20147698141287	39.0	36.0	40.0	32.0	41.0
36	37.10794501837308	39.0	36.0	40.0	32.0	41.0
37	37.025518446978	39.0	35.0	40.0	31.0	41.0
38	36.932353435842025	39.0	35.0	40.0	31.0	41.0
39	36.82275203734755	39.0	35.0	40.0	31.0	41.0
40	36.706520021813255	39.0	35.0	40.0	31.0	41.0
41	36.590976041098614	39.0	35.0	40.0	31.0	41.0
42	36.469927781826705	39.0	35.0	40.0	30.0	41.0
43	35.53174898451157	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	1.0
16	1.0
17	2.0
18	3.0
19	8.0
20	31.0
21	56.0
22	97.0
23	157.0
24	262.0
25	423.0
26	619.0
27	978.0
28	1367.0
29	1894.0
30	2659.0
31	3464.0
32	4419.0
33	6000.0
34	8575.0
35	12240.0
36	19661.0
37	34841.0
38	57984.0
39	40468.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.50826406266723	18.21763305828929	12.846374566155822	25.427728312887655
2	19.59930890724781	20.634419069267267	33.187232112368825	26.57903991111609
3	20.553383857174165	22.280606082227806	29.25320191018852	27.912808150409507
4	15.34011854585115	15.799318081045405	32.53538282767021	36.325180545433234
5	15.767209789461345	35.37008628466294	32.38962137698702	16.473082548888694
6	34.73556528431128	33.673443384927445	15.466513090499515	16.12447824026176
7	29.952958804552242	29.776618028550892	20.20936644734495	20.06105671955191
8	29.167070143875723	31.661833434414994	18.894965114086364	20.276131307622915
9	27.10806223912013	13.45031624118933	19.45660538909643	39.985016130594104
10	18.646762923587364	26.335424619414816	30.90346616652481	24.11434629047301
11	36.719653842037395	21.13948759243875	20.020793941216343	22.120064624307506
12	22.39374958590497	25.95980857342351	27.851649499773202	23.79479234089832
13	32.14957367323952	18.85419268032883	23.417137673219138	25.579095973212514
14	23.33763142739194	20.26186095580778	24.857423895704116	31.543083721096167
15	26.780353802793933	26.215655595252052	21.814271371125983	25.18971923082804
16	25.47104902375504	24.840605266779132	23.778993022817275	25.909352686648557
17	23.868692377083853	26.200875588014945	24.947632905392663	24.98279912950854
18	24.100585594079842	23.914051709639114	26.30637426036257	25.678988435918477
19	24.6739479437952	24.82072870532233	26.833867622100698	23.67145572878177
20	25.374214493580887	24.16225390013812	26.434807426698807	24.028724179582184
21	26.11576313254609	23.844738572251302	25.636177380473065	24.40332091472955
22	26.055623792753718	24.44918990270678	25.542400782830725	23.952785521708773
23	25.022552252422138	24.337065709873553	25.505195937026976	25.13518610067733
24	24.646936206430833	24.780975582408736	25.722818802207826	24.84926940895261
25	25.016946042780475	24.35439399422051	25.807931257676685	24.82072870532233
26	25.10562608620312	24.456834734036317	25.784996763688074	24.652542416072492
27	24.71777831008455	24.702488647425476	25.740656741976746	24.839076300513224
28	24.150531825432825	24.370193312301552	26.38791912787764	25.09135573438798
29	24.05012970730489	25.01388811024866	26.458761231531362	24.477220950915086
30	24.327891912278112	25.279928240516586	26.244705954304294	24.147473892901008
31	24.556217541320315	24.957826013832047	25.73912777571084	24.746828669136796
32	23.9920289892004	24.99044396083808	26.26865975913685	24.748867290824673
33	23.825371666216473	24.704527269113353	26.607070959324403	24.863030105345775
34	24.437977483423456	24.331459500231894	26.294652185657274	24.935910830687373
35	24.001712442217816	24.402811259307583	26.76659310640076	24.82888319207384
36	23.736691622793828	24.553159608788498	26.56222128219111	25.147927486226564
37	23.67706193842343	23.74892335292109	27.15393122709736	25.42008348155812
38	23.238758275529914	23.585323962468973	27.987217842017014	25.1886999199841
39	22.928378123550665	23.064456121216445	28.75272028581476	25.254445469418123
40	23.00176850431423	23.057320945308877	28.71347681832313	25.22743373205376
41	22.12414186768326	23.091467858580813	29.469805464525432	25.314584809210494
42	21.711320975888203	23.21531412611933	29.661435903185858	25.41192899480661
43	21.37800632992034	22.8086090993879	29.92034085754621	25.89304371314554
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	29.0
1	36.5
2	44.0
3	106.5
4	169.0
5	169.0
6	216.5
7	264.0
8	277.5
9	291.0
10	412.0
11	533.0
12	533.0
13	981.5
14	1430.0
15	2161.0
16	2892.0
17	2524.5
18	2157.0
19	2157.0
20	2376.0
21	2595.0
22	2047.5
23	1500.0
24	1623.0
25	1746.0
26	1746.0
27	2031.0
28	2316.0
29	2733.0
30	3150.0
31	3684.5
32	4219.0
33	4219.0
34	4758.5
35	5298.0
36	6056.0
37	6814.0
38	7555.5
39	8297.0
40	8297.0
41	9105.5
42	9914.0
43	10637.5
44	11361.0
45	12037.0
46	12713.0
47	12713.0
48	13370.5
49	14028.0
50	13996.0
51	13964.0
52	13734.5
53	13505.0
54	13505.0
55	13391.0
56	13277.0
57	12780.5
58	12284.0
59	11761.0
60	11238.0
61	11238.0
62	10646.0
63	10054.0
64	9380.0
65	8706.0
66	7748.0
67	6790.0
68	6790.0
69	6006.5
70	5223.0
71	4486.5
72	3750.0
73	2957.0
74	2164.0
75	2164.0
76	1809.5
77	1455.0
78	1159.5
79	864.0
80	707.0
81	550.0
82	550.0
83	441.0
84	332.0
85	251.5
86	171.0
87	130.5
88	90.0
89	90.0
90	57.0
91	24.0
92	14.5
93	5.0
94	4.5
95	4.0
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	196211.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.64629142084907
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.61857661146068	60.48634914331543
2	8.547143685339096	12.076480072206712
3	2.2866670767982553	4.8463364606985175
4	1.1415868401563607	3.2259550636756997
5	0.6182093167217237	2.1837097774103436
6	0.40259131279666754	1.7064949924401347
7	0.28436909940328664	1.406273558727029
8	0.21313257372086264	1.204562073148773
9	0.16671397895389062	1.059995190699527
>10	0.6993104436919643	8.046005719495648
>50	0.015781135241611996	0.7574207027023925
>100	0.0029589628578022492	0.528173774285569
>500	9.863209526007497E-4	0.6431456380812404
>1k	0.0019726419052014995	1.8290978331129533
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1487	0.7578576124682103	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1138	0.5799878702009572	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	923	0.4704119544775777	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	489	0.24922150134294202	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	5.096554219692067E-4	0.0
11	0.0	0.0	0.0	5.096554219692067E-4	0.0
12	0.0	0.0	0.0	5.096554219692067E-4	0.0
13	0.0	0.0	0.0	0.0010193108439384133	0.0
14	0.0	0.0	0.0	0.0010193108439384133	0.0
15	0.0	0.0	0.0	0.0010193108439384133	0.0
16	0.0	0.0	0.0	0.0010193108439384133	0.0
17	0.0	0.0	0.0	0.0015289662659076198	0.0
18	0.0	0.0	0.0	0.0015289662659076198	0.0
19	0.0	0.0	0.0	0.0015289662659076198	0.0
20	0.0	0.0	0.0	0.0020386216878768267	0.0
21	0.0	0.0	0.0	0.0020386216878768267	0.0
22	0.0	0.0	0.0	0.0035675879537844465	0.0
23	0.0	0.0	0.0	0.005096554219692066	0.0
24	0.0	0.0	0.0	0.007135175907568893	0.0
25	0.0	0.0	0.0	0.010193108439384133	0.0
26	0.0	0.0	0.0	0.013760696393168579	0.0
27	0.0	0.0	0.0	0.04739795424313622	0.0
28	0.0	0.0	0.0	0.13709730850971658	0.0
29	0.0	0.0	0.0	0.2767428941292792	0.0
30	0.0	0.0	0.0	0.45919953519425516	0.0
31	0.0	0.0	0.0	0.9418432197990938	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACACCG	20	0.0018382185	37.0	5
GATCGGA	25	0.0054853777	29.6	16
ACACCGC	25	0.0054853777	29.6	6
CCTTATT	35	8.8430947E-4	26.42857	27
GCCTTAT	35	8.8430947E-4	26.42857	26
CTTATTG	40	0.0019255151	23.125	28
TATTCCA	40	0.0019255151	23.125	5
AGATCGG	45	0.00381468	20.555553	15
CTACAAG	45	0.00381468	20.555553	1
TTTGAAC	45	0.00381468	20.555553	3
ACAAGGT	45	0.00381468	20.555553	3
TCTACAC	65	6.8649184E-5	19.923075	3
ATCTCGT	50	0.007014614	18.5	37
ATCTCCG	50	0.007014614	18.5	10
GCGCGGC	50	0.007014614	18.5	17
GGTATCA	495	0.0	17.565657	1
CTTATAC	210	0.0	16.738094	37
CAGGAGT	90	4.4158027E-5	16.444443	4
CTGAGCT	80	3.3647157E-4	16.1875	4
CTACACC	80	3.3647157E-4	16.1875	4
>>END_MODULE