FastQCFastQC Report
Fri 10 Feb 2017
ERR1632280.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632280.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences950143
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4000.021.2750021
TAGTAGT555.142191E-420.1818184
CTTATAC7300.014.44520637
GTCCTAT908.272154E-414.3888881
ATTCGTG908.272154E-414.38888811
TCGTATG908.272154E-414.38888830
TAGTCCG1551.2091368E-714.322585
TCTTATA12050.014.27800937
GTATTAG2353.6379788E-1214.1702141
GGATAGA1051.6551543E-414.0952381
CTAGATA800.0062975613.8750013
GTATCAA6300.013.8015872
GTACACT1356.5636195E-613.7037044
CGTATGC950.001244795213.63157931
ATTCCGT950.001244795213.63157929
CTCGATT950.001244795213.63157925
TAAGAGT1652.5927693E-713.4545454
AGTCCGC1254.842655E-513.326
ATAGTTC1409.553891E-613.2142868
ATACACT850.0094031613.0588244
Produced by FastQC (version 0.11.5)