##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632280.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	950143
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.848181800002735	34.0	31.0	34.0	31.0	34.0
2	33.006297999353784	34.0	33.0	34.0	31.0	34.0
3	33.001880769526274	34.0	33.0	34.0	31.0	34.0
4	36.42821133239944	37.0	37.0	37.0	35.0	37.0
5	36.34909271551756	37.0	37.0	37.0	35.0	37.0
6	36.38087214240383	37.0	37.0	37.0	35.0	37.0
7	36.37753580250552	37.0	37.0	37.0	35.0	37.0
8	36.360746750752256	37.0	37.0	37.0	35.0	37.0
9	38.15519769129489	39.0	39.0	39.0	37.0	39.0
10	38.095348805390344	39.0	38.0	39.0	37.0	39.0
11	38.19523482254776	39.0	39.0	39.0	37.0	39.0
12	38.16645073425789	39.0	39.0	39.0	37.0	39.0
13	38.19044185980426	39.0	39.0	39.0	37.0	39.0
14	39.63355831701123	41.0	39.0	41.0	37.0	41.0
15	39.652212351193455	41.0	39.0	41.0	37.0	41.0
16	39.54046601406315	41.0	39.0	41.0	37.0	41.0
17	39.578663422242755	41.0	39.0	41.0	37.0	41.0
18	39.59449051353322	41.0	39.0	41.0	37.0	41.0
19	39.63786082726495	41.0	39.0	41.0	37.0	41.0
20	39.61083121172287	41.0	39.0	41.0	37.0	41.0
21	39.59033219210161	41.0	39.0	41.0	37.0	41.0
22	39.569697403443485	41.0	39.0	41.0	37.0	41.0
23	39.52934874013701	41.0	39.0	41.0	37.0	41.0
24	39.48225477638629	41.0	39.0	41.0	37.0	41.0
25	39.423323647072074	41.0	39.0	41.0	37.0	41.0
26	39.33498957525341	41.0	39.0	41.0	36.0	41.0
27	39.27758979437832	40.0	39.0	41.0	36.0	41.0
28	39.22163611161688	40.0	39.0	41.0	36.0	41.0
29	39.0852303284874	40.0	39.0	41.0	36.0	41.0
30	39.04824536938124	40.0	39.0	41.0	35.0	41.0
31	38.98959630287231	40.0	39.0	41.0	35.0	41.0
32	38.93292272847351	40.0	38.0	41.0	35.0	41.0
33	38.79787884560535	40.0	38.0	41.0	35.0	41.0
34	38.791715562815284	40.0	38.0	41.0	35.0	41.0
35	38.777373511145164	40.0	38.0	41.0	35.0	41.0
36	38.74765693164082	40.0	38.0	41.0	35.0	41.0
37	38.71332841477546	40.0	38.0	41.0	35.0	41.0
38	38.64155500803563	40.0	38.0	41.0	35.0	41.0
39	38.44377741034771	40.0	38.0	41.0	35.0	41.0
40	38.45426425285457	40.0	38.0	41.0	34.0	41.0
41	38.452515042472555	40.0	38.0	41.0	34.0	41.0
42	38.32925570151019	40.0	38.0	41.0	34.0	41.0
43	37.48052240557474	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	1.0
12	0.0
13	0.0
14	1.0
15	1.0
16	1.0
17	7.0
18	7.0
19	14.0
20	42.0
21	78.0
22	197.0
23	303.0
24	545.0
25	783.0
26	1200.0
27	1746.0
28	2432.0
29	3573.0
30	4898.0
31	6917.0
32	9526.0
33	13667.0
34	20483.0
35	31892.0
36	51663.0
37	96089.0
38	228771.0
39	475303.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.92675628826398	17.150576281675495	11.686030418579097	27.23663701148143
2	20.58963755981994	19.095230928397093	32.300611592149814	28.014519919633152
3	21.658739789694813	20.297260517627347	27.625525841899588	30.41847385077825
4	16.361852900037153	14.74346493106827	31.309602870304786	37.58507929858979
5	17.68712709560561	33.00734731508836	32.082539154632514	17.22298643467352
6	37.84598739347656	31.3945374538359	14.523498041873697	16.235977110813845
7	31.585350836663533	27.45618291141439	19.777654521477295	21.180811730444784
8	28.377307415831094	31.12426234787816	18.83095491941739	21.667475316873354
9	27.068978038042697	13.723723692117924	18.581413534594265	40.625884735245116
10	18.836743521764618	24.850048887378005	31.540515480301384	24.77269211055599
11	38.234560481948506	20.06203276769918	19.693561916469417	22.009844833882898
12	23.218925993245225	23.262077392560908	27.353566778895388	26.165429835298475
13	32.540049234694145	18.218310296450113	22.098463073453154	27.143177395402585
14	24.45305601367373	19.154800908915817	22.761521160498997	33.63062191691145
15	27.289997400391307	25.422910025122537	20.186645589137637	27.10044698534852
16	27.376615940968886	23.7979967225986	22.068151846616775	26.757235489815745
17	25.853371545125313	24.302657599961268	23.429525871368835	26.414444983544584
18	26.116910822897182	23.464994216660017	24.086795356067455	26.331299604375342
19	26.696823530773788	23.60991976997147	23.80967917460845	25.883577524646288
20	26.72092516600133	23.338486943544286	23.72905973100891	26.211528159445475
21	27.030878509866408	23.184404873792683	23.315648276101598	26.46906834023931
22	27.160964191705876	23.089892784559797	23.06810659027115	26.681036433463174
23	26.73944869351245	23.170406980843936	23.311754125431648	26.778390200211966
24	26.820383879058205	23.38858466567664	22.986539920832968	26.804491534432184
25	27.1256010937301	22.881503100059675	23.184931110369703	26.807964695840518
26	26.744079575390227	23.349222169715507	23.317963717040488	26.588734537853774
27	26.57757832242094	23.207348788550778	23.31859520093291	26.89647768809537
28	26.363715777519804	23.224714595592452	23.538351595496678	26.873218031391065
29	26.165850824560096	23.46425748545219	23.882510316868093	26.48738137311962
30	26.19479383629622	23.606762350509346	23.54361396126688	26.654829851927552
31	26.834276524691546	23.179353002653286	23.365430256287738	26.620940216367433
32	26.16364063093661	23.375639245881935	23.4208955915057	27.03982453167576
33	26.079232283982517	23.05168800906811	24.00017681548988	26.868902891459495
34	26.544109676122435	22.629856768928466	23.64591435183967	27.180119203109427
35	25.95440896791325	22.838351700743996	24.33991514961432	26.867324181728435
36	26.003454216891562	22.53218726023346	24.38085635530652	27.08350216756846
37	25.827375458220498	21.880495883251257	25.118955778235485	27.173172880292757
38	25.288824945297705	21.89828267955455	25.91515171926752	26.89774065588022
39	25.058754313824338	21.73757002893249	26.321932593304375	26.881743063938796
40	24.89951512561793	21.563069979992484	26.934156227010043	26.603258667379542
41	24.21140817750591	21.707785038673126	27.213166860146316	26.867639923674645
42	23.994388213142653	22.01173928556017	27.22221812927107	26.771654372026106
43	23.58381843575125	21.805770289314346	27.452814997321457	27.15759627761295
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	29.0
1	39.0
2	49.0
3	88.5
4	128.0
5	128.0
6	162.5
7	197.0
8	191.0
9	185.0
10	241.0
11	297.0
12	297.0
13	573.0
14	849.0
15	1151.0
16	1453.0
17	1553.5
18	1654.0
19	1654.0
20	2271.0
21	2888.0
22	3087.5
23	3287.0
24	4145.0
25	5003.0
26	5003.0
27	6222.0
28	7441.0
29	9433.0
30	11425.0
31	13639.0
32	15853.0
33	15853.0
34	19091.5
35	22330.0
36	25741.5
37	29153.0
38	32646.0
39	36139.0
40	36139.0
41	40424.0
42	44709.0
43	47614.5
44	50520.0
45	54366.5
46	58213.0
47	58213.0
48	61785.0
49	65357.0
50	67901.0
51	70445.0
52	72514.0
53	74583.0
54	74583.0
55	74811.5
56	75040.0
57	74373.5
58	73707.0
59	71529.0
60	69351.0
61	69351.0
62	64709.5
63	60068.0
64	54359.5
65	48651.0
66	43421.5
67	38192.0
68	38192.0
69	33169.5
70	28147.0
71	24068.5
72	19990.0
73	16550.0
74	13110.0
75	13110.0
76	10872.5
77	8635.0
78	7143.0
79	5651.0
80	4637.0
81	3623.0
82	3623.0
83	2759.5
84	1896.0
85	1460.5
86	1025.0
87	771.0
88	517.0
89	517.0
90	395.5
91	274.0
92	169.0
93	64.0
94	37.0
95	10.0
96	10.0
97	6.5
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	950143.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.26843982589448
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.5168330177695	63.24704980697531
2	7.20237779820367	10.410092130256805
3	2.081421081562203	4.5126316255567875
4	0.9140116422856914	2.6421678148276198
5	0.5330413752680296	1.9261034276634819
6	0.33280331146338443	1.443070565301001
7	0.24487201270443998	1.2387562820623543
8	0.1901526186801495	1.0993626464658113
9	0.1427502071332084	0.928470127890622
>10	0.8041512826202725	10.29009351721088
>50	0.030861146723095246	1.4812703368800522
>100	0.006432135978174233	0.64982568170208
>500	2.923696082443977E-4	0.1311060372072514
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.0524731540410233E-4	0.0
10	0.0	0.0	0.0	1.0524731540410233E-4	0.0
11	0.0	0.0	0.0	1.0524731540410233E-4	0.0
12	0.0	0.0	0.0	1.0524731540410233E-4	0.0
13	0.0	0.0	0.0	1.0524731540410233E-4	0.0
14	0.0	0.0	0.0	2.1049463080820467E-4	0.0
15	0.0	0.0	0.0	3.15741946212307E-4	0.0
16	0.0	1.0524731540410233E-4	0.0	4.2098926161640934E-4	1.0524731540410233E-4
17	0.0	1.0524731540410233E-4	0.0	6.31483892424614E-4	1.0524731540410233E-4
18	0.0	1.0524731540410233E-4	0.0	6.31483892424614E-4	1.0524731540410233E-4
19	0.0	1.0524731540410233E-4	0.0	6.31483892424614E-4	1.0524731540410233E-4
20	0.0	1.0524731540410233E-4	0.0	8.419785232328187E-4	1.0524731540410233E-4
21	0.0	1.0524731540410233E-4	0.0	9.47225838636921E-4	1.0524731540410233E-4
22	0.0	1.0524731540410233E-4	0.0	0.0010524731540410234	1.0524731540410233E-4
23	0.0	2.1049463080820467E-4	0.0	0.0016839570464656374	1.0524731540410233E-4
24	0.0	2.1049463080820467E-4	0.0	0.0031574194621230697	1.0524731540410233E-4
25	0.0	2.1049463080820467E-4	0.0	0.004630881877780503	1.0524731540410233E-4
26	0.0	2.1049463080820467E-4	0.0	0.00989324764798562	1.0524731540410233E-4
27	0.0	3.15741946212307E-4	0.0	0.030837463413401982	1.0524731540410233E-4
28	0.0	3.15741946212307E-4	0.0	0.09735376674879466	1.0524731540410233E-4
29	0.0	3.15741946212307E-4	0.0	0.17039540363924166	1.0524731540410233E-4
30	0.0	3.15741946212307E-4	0.0	0.2751164824663235	1.0524731540410233E-4
31	0.0	3.15741946212307E-4	0.0	0.5488647498323936	1.0524731540410233E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	400	0.0	21.275002	1
TAGTAGT	55	5.142191E-4	20.181818	4
CTTATAC	730	0.0	14.445206	37
GTCCTAT	90	8.272154E-4	14.388888	1
ATTCGTG	90	8.272154E-4	14.388888	11
TCGTATG	90	8.272154E-4	14.388888	30
TAGTCCG	155	1.2091368E-7	14.32258	5
TCTTATA	1205	0.0	14.278009	37
GTATTAG	235	3.6379788E-12	14.170214	1
GGATAGA	105	1.6551543E-4	14.095238	1
CTAGATA	80	0.00629756	13.875001	3
GTATCAA	630	0.0	13.801587	2
GTACACT	135	6.5636195E-6	13.703704	4
CGTATGC	95	0.0012447952	13.631579	31
ATTCCGT	95	0.0012447952	13.631579	29
CTCGATT	95	0.0012447952	13.631579	25
TAAGAGT	165	2.5927693E-7	13.454545	4
AGTCCGC	125	4.842655E-5	13.32	6
ATAGTTC	140	9.553891E-6	13.214286	8
ATACACT	85	0.00940316	13.058824	4
>>END_MODULE