FastQCFastQC Report
Fri 10 Feb 2017
ERR1632279.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632279.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences227471
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7070.31080885035894684No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6190.2721226002435475No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4600.2022235801486783No Hit
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT4290.18859546931257173Illumina PCR Primer Index 10 (95% over 22bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2730.12001529865345473No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT2510.1103437361246049No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACG200.001838834337.04
ACGGCGA200.001838834337.012
TACTAAG200.001838834337.02
CCTCTAG303.5904866E-430.8333321
GCGACTA250.00548719329.615
CAATGGG250.00548719329.61
TCGAAAG250.00548719329.625
TGGTACT250.00548719329.64
GGTATCA6150.027.6747971
ACATAAA358.847447E-426.42857232
GGGGCTC358.847447E-426.4285725
GCTTAGA509.049294E-625.9000021
CTTATAC2150.024.95349137
TAGCCCC551.8939734E-523.5454544
ATGGGCC400.001926454223.12512
TTAACAA400.001926454223.12526
CTTATTG603.7093978E-521.58333228
GCAGTCG705.072294E-621.1428599
CAGTCGG705.072294E-621.14285910
CGGTGAT806.9097405E-720.812514
Produced by FastQC (version 0.11.5)