FastQCFastQC Report
Fri 10 Feb 2017
ERR1632278.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632278.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences302057
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5040.16685592454404302No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4270.14136404718314755No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3160.10461601618237618No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAA200.001839788937.010
CCTAGTA250.00549000929.62
AGGTTAC250.00549000929.67
CGCCTTA604.3006366E-827.74999825
TAACAAT358.854204E-426.4285731
CATGGAC551.8966452E-523.5454543
GTATTAA502.695716E-422.21
AATAGAC502.695716E-422.218
GGTATCA2550.021.7647061
TTTGAAC705.081405E-621.1428573
CTTATTG1001.2803866E-820.3528
TAGGGTG555.1310397E-420.1818185
TATACAA759.228737E-619.7333345
CCTTATT902.1415472E-618.527
GTTCTAC500.007023172518.51
GTTTCAA609.2153426E-418.522
CGACCAA500.007023172518.514
CGGTGAT1002.8575232E-718.514
TTCCTCG1002.8575232E-718.520
GTAAACG609.2153426E-418.527
Produced by FastQC (version 0.11.5)