##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632278.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	302057
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.729263019893594	34.0	31.0	34.0	31.0	34.0
2	32.890967598830684	34.0	31.0	34.0	31.0	34.0
3	32.8860943464313	34.0	31.0	34.0	31.0	34.0
4	36.36479538630126	37.0	37.0	37.0	35.0	37.0
5	36.275448011468036	37.0	37.0	37.0	35.0	37.0
6	36.314821374773636	37.0	37.0	37.0	35.0	37.0
7	36.3017675471848	37.0	37.0	37.0	35.0	37.0
8	36.28246324369242	37.0	37.0	37.0	35.0	37.0
9	38.07189040479115	39.0	38.0	39.0	37.0	39.0
10	38.0062008164022	39.0	38.0	39.0	35.0	39.0
11	38.110644679646555	39.0	38.0	39.0	37.0	39.0
12	38.075773777796904	39.0	38.0	39.0	37.0	39.0
13	38.10013672916039	39.0	38.0	39.0	37.0	39.0
14	39.48141575927722	40.0	39.0	41.0	37.0	41.0
15	39.510519537703146	40.0	39.0	41.0	37.0	41.0
16	39.406847052046466	40.0	39.0	41.0	37.0	41.0
17	39.45047126866783	40.0	39.0	41.0	37.0	41.0
18	39.455304793466134	40.0	39.0	41.0	37.0	41.0
19	39.50276603422533	41.0	39.0	41.0	37.0	41.0
20	39.48247185133932	40.0	39.0	41.0	37.0	41.0
21	39.458704813992064	40.0	39.0	41.0	37.0	41.0
22	39.427111439231666	40.0	39.0	41.0	37.0	41.0
23	39.37841864283894	40.0	39.0	41.0	37.0	41.0
24	39.31989988644527	40.0	39.0	41.0	36.0	41.0
25	39.25646815005115	40.0	39.0	41.0	36.0	41.0
26	39.16779945506974	40.0	39.0	41.0	36.0	41.0
27	39.09409151252909	40.0	39.0	41.0	36.0	41.0
28	39.01673525195576	40.0	39.0	41.0	35.0	41.0
29	38.88211165442284	40.0	38.0	41.0	35.0	41.0
30	38.82322210708575	40.0	38.0	41.0	35.0	41.0
31	38.75501643729495	40.0	38.0	41.0	35.0	41.0
32	38.67205527433564	40.0	38.0	41.0	35.0	41.0
33	38.544264824188815	40.0	38.0	41.0	35.0	41.0
34	38.528632013163076	40.0	38.0	41.0	35.0	41.0
35	38.482538064007784	40.0	38.0	41.0	35.0	41.0
36	38.44659782756235	40.0	38.0	41.0	35.0	41.0
37	38.4125314096346	40.0	38.0	41.0	34.0	41.0
38	38.333420513346816	40.0	38.0	41.0	34.0	41.0
39	38.166346086996825	40.0	38.0	41.0	34.0	41.0
40	38.138632774608766	40.0	38.0	41.0	34.0	41.0
41	38.13761309951433	40.0	37.0	41.0	34.0	41.0
42	38.00742244013547	40.0	37.0	41.0	34.0	41.0
43	37.14039072095664	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	0.0
15	0.0
16	2.0
17	1.0
18	6.0
19	7.0
20	12.0
21	42.0
22	91.0
23	124.0
24	252.0
25	327.0
26	456.0
27	675.0
28	961.0
29	1319.0
30	1862.0
31	2563.0
32	3517.0
33	5099.0
34	7538.0
35	11466.0
36	18556.0
37	33997.0
38	78042.0
39	135140.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.46298877364206	17.272567760389595	12.06328606852349	27.201157397444852
2	20.301135216200915	19.681384639323042	32.32038986019195	27.697090284284094
3	21.01854947907183	20.406744422410338	28.819726078190538	29.754980020327288
4	15.78443803652953	15.160714699543465	32.016804775257654	37.03804248866936
5	16.947463558202593	33.74296904226686	32.44486967691528	16.86469772261527
6	36.605342700218834	32.47036155427618	15.042525086324767	15.881770659180223
7	31.449031143128614	28.267181359809573	19.660527648755036	20.623259848306777
8	28.841245195443243	30.813389525817975	19.446660729597394	20.898704549141385
9	27.62822910907544	13.303780412306288	18.30912708528523	40.758863393333044
10	18.509420407406548	25.077717119616494	31.122271624229864	25.29059084874709
11	38.07427075022264	20.05548621617774	19.5608775827079	22.30936545089172
12	23.520395157205428	23.75743651032752	27.04853719662183	25.67363113584522
13	32.577295013855	17.65958080759592	22.820196188136677	26.942927990412407
14	24.59337144975948	19.20101172957422	23.01088867332987	33.19472814733643
15	27.431246420377608	25.11843791072546	20.4024405989598	27.047875069937135
16	27.276308776158142	23.87893675696971	22.096822785103473	26.747931681768673
17	25.73620210754924	24.41592149826026	23.574689545350715	26.273186848839792
18	25.89776101861569	22.96288448868922	24.61753907375098	26.521815418944104
19	26.755546138642707	23.380024300049328	24.073933065613442	25.79049649569452
20	27.213075677769428	22.791393677352286	23.701817868812842	26.29371277606544
21	27.588170444651176	23.00095677305939	23.09895152239478	26.311921259894653
22	27.767937839546843	23.067500504871596	22.821189378163727	26.34337227741784
23	26.820765617085517	22.868200372777324	23.236673872812087	27.074360137325076
24	26.37217478820223	23.233032176046244	23.38896301029276	27.00583002545877
25	26.8280490106172	22.714255918584904	23.15125953048597	27.30643554031193
26	26.306293183074718	23.469411402483637	23.47768798604237	26.746607428399276
27	26.892275299032963	23.0420086275107	23.14099656687314	26.924719506583195
28	26.275504292236235	23.22641090919926	23.838215965860748	26.659868832703758
29	25.756065908090193	23.474046289276526	24.232512406598754	26.537375396034523
30	25.892132941795754	23.8143794052116	24.129551707128126	26.163935945864523
31	26.515525215439474	23.555818934836804	23.29361676769616	26.63503908202756
32	25.42301618568681	23.44954760194268	24.15471252114667	26.972723691223845
33	25.494525867634255	23.2065471086583	24.404996407962738	26.89393061574471
34	26.373830104913974	22.60798458569078	24.278861274527657	26.73932403486759
35	25.712034483557737	22.806291527758006	25.151875308302742	26.329798680381515
36	25.17869143903303	22.88806417331828	24.854249363530727	27.07899502411796
37	25.437582972750178	21.844883581575665	25.514389668175212	27.20314377749895
38	24.818494522557	21.816081070791274	26.46487252406003	26.900551882591696
39	24.595688893155927	21.37046981198913	26.938293103619515	27.09554819123543
40	23.80908239173401	21.479720714964394	27.927841433901545	26.78335545940005
41	23.17377183776572	21.35027494810582	28.702529654998894	26.773423559129565
42	23.40750255746432	21.231754271544776	28.59857576550121	26.76216740548969
43	22.352403685397128	20.899035612483736	29.359359326219884	27.38920137589925
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	9.0
1	8.0
2	7.0
3	27.0
4	47.0
5	47.0
6	70.0
7	93.0
8	100.5
9	108.0
10	150.0
11	192.0
12	192.0
13	336.0
14	480.0
15	756.5
16	1033.0
17	959.5
18	886.0
19	886.0
20	1077.5
21	1269.0
22	1236.0
23	1203.0
24	1529.0
25	1855.0
26	1855.0
27	2197.5
28	2540.0
29	3082.0
30	3624.0
31	4495.0
32	5366.0
33	5366.0
34	6395.5
35	7425.0
36	8494.0
37	9563.0
38	10633.0
39	11703.0
40	11703.0
41	12661.0
42	13619.0
43	14672.0
44	15725.0
45	16949.0
46	18173.0
47	18173.0
48	19075.5
49	19978.0
50	21176.5
51	22375.0
52	23678.5
53	24982.0
54	24982.0
55	24495.5
56	24009.0
57	23728.0
58	23447.0
59	22264.5
60	21082.0
61	21082.0
62	19723.5
63	18365.0
64	16719.0
65	15073.0
66	13368.0
67	11663.0
68	11663.0
69	10152.0
70	8641.0
71	7580.5
72	6520.0
73	5370.5
74	4221.0
75	4221.0
76	3430.0
77	2639.0
78	2235.5
79	1832.0
80	1441.0
81	1050.0
82	1050.0
83	823.5
84	597.0
85	472.0
86	347.0
87	270.0
88	193.0
89	193.0
90	144.0
91	95.0
92	56.0
93	17.0
94	13.0
95	9.0
96	9.0
97	5.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	302057.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.69744725698587
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.85326314064551	71.36764068252349
2	4.801475478865984	7.461247755498017
3	1.1882662230268988	2.7697575657267084
4	0.5755210450959338	1.7886606418650683
5	0.346592917870613	1.3464692477948395
6	0.21422681809666463	0.9986926140058505
7	0.16130384956242427	0.877302814060767
8	0.12035116444645312	0.7480782601516099
9	0.08759627864571291	0.6125406515985166
>10	0.601857923163591	8.886267365943114
>50	0.04185711842121884	2.0993226380561376
>100	0.007260928705715585	0.8767642127316745
>500	4.271134532773873E-4	0.16725555004419526
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	504	0.16685592454404302	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	427	0.14136404718314755	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	316	0.10461601618237618	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	3.310633423492917E-4	0.0
10	0.0	0.0	0.0	3.310633423492917E-4	0.0
11	0.0	0.0	0.0	3.310633423492917E-4	0.0
12	0.0	0.0	0.0	3.310633423492917E-4	0.0
13	3.310633423492917E-4	0.0	0.0	3.310633423492917E-4	0.0
14	3.310633423492917E-4	0.0	0.0	6.621266846985834E-4	0.0
15	3.310633423492917E-4	0.0	0.0	6.621266846985834E-4	0.0
16	3.310633423492917E-4	0.0	0.0	0.0013242533693971668	0.0
17	3.310633423492917E-4	0.0	0.0	0.0013242533693971668	0.0
18	3.310633423492917E-4	0.0	0.0	0.0016553167117464584	0.0
19	3.310633423492917E-4	0.0	0.0	0.0019863800540957503	0.0
20	3.310633423492917E-4	0.0	0.0	0.0019863800540957503	0.0
21	3.310633423492917E-4	0.0	0.0	0.0026485067387943336	0.0
22	3.310633423492917E-4	0.0	0.0	0.003310633423492917	0.0
23	3.310633423492917E-4	0.0	0.0	0.005297013477588667	0.0
24	3.310633423492917E-4	0.0	0.0	0.006621266846985834	0.0
25	3.310633423492917E-4	0.0	0.0	0.007945520216383001	0.0
26	3.310633423492917E-4	0.0	0.0	0.010594026955177334	0.0
27	3.310633423492917E-4	0.0	0.0	0.040389727766613585	0.0
28	3.310633423492917E-4	0.0	0.0	0.11289259974110846	0.0
29	3.310633423492917E-4	0.0	0.0	0.23836560649149002	0.0
30	3.310633423492917E-4	0.0	0.0	0.38866836391806847	0.0
31	3.310633423492917E-4	0.0	0.0	0.8587783100540627	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTCTAA	20	0.0018397889	37.0	10
CCTAGTA	25	0.005490009	29.6	2
AGGTTAC	25	0.005490009	29.6	7
CGCCTTA	60	4.3006366E-8	27.749998	25
TAACAAT	35	8.854204E-4	26.42857	31
CATGGAC	55	1.8966452E-5	23.545454	3
GTATTAA	50	2.695716E-4	22.2	1
AATAGAC	50	2.695716E-4	22.2	18
GGTATCA	255	0.0	21.764706	1
TTTGAAC	70	5.081405E-6	21.142857	3
CTTATTG	100	1.2803866E-8	20.35	28
TAGGGTG	55	5.1310397E-4	20.181818	5
TATACAA	75	9.228737E-6	19.733334	5
CCTTATT	90	2.1415472E-6	18.5	27
GTTCTAC	50	0.0070231725	18.5	1
GTTTCAA	60	9.2153426E-4	18.5	22
CGACCAA	50	0.0070231725	18.5	14
CGGTGAT	100	2.8575232E-7	18.5	14
TTCCTCG	100	2.8575232E-7	18.5	20
GTAAACG	60	9.2153426E-4	18.5	27
>>END_MODULE