FastQCFastQC Report
Fri 10 Feb 2017
ERR1632277.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632277.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences567591
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5000.022.571
CGTTTAT502.699767E-422.231
TCGTTTA555.138685E-420.18181830
GTGCTAC555.138685E-420.1818181
CGGTGAT701.2178183E-418.514
GTATCAA6150.017.7479692
GTAGAAC752.064944E-417.2666663
CGGACCA752.064944E-417.2666669
GTATTAG650.001578601917.0769231
GTCCTAA1107.790477E-716.8181821
TCGGTGA803.3787914E-416.187513
AGTCGGT803.3787914E-416.187511
TCTTATA5450.015.95412837
CGCCTTA700.00259032715.85714225
CTTATAC3100.015.51612937
TGCACCG1101.4492152E-515.1363645
AAGACGG750.004101002614.85
GTCGGTG1001.0918152E-414.812
TAGAACT1803.3160177E-914.3888894
GACGGAC908.264917E-414.3888897
Produced by FastQC (version 0.11.5)