##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632277.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	567591
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.97548234556221	33.0	31.0	34.0	30.0	34.0
2	32.15627097681253	33.0	31.0	34.0	30.0	34.0
3	32.17830973359338	34.0	31.0	34.0	30.0	34.0
4	35.90546009362375	37.0	35.0	37.0	35.0	37.0
5	35.490312566619274	37.0	35.0	37.0	33.0	37.0
6	35.48296748891367	37.0	35.0	37.0	33.0	37.0
7	35.477488191320866	37.0	35.0	37.0	33.0	37.0
8	35.434703862464346	37.0	35.0	37.0	33.0	37.0
9	37.12655591790568	39.0	37.0	39.0	33.0	39.0
10	36.9140278827536	39.0	37.0	39.0	33.0	39.0
11	37.06642282911463	39.0	37.0	39.0	33.0	39.0
12	37.01046352038704	39.0	37.0	39.0	33.0	39.0
13	37.060291653673154	39.0	37.0	39.0	33.0	39.0
14	38.22524317686503	40.0	38.0	41.0	34.0	41.0
15	38.20614139406721	40.0	38.0	41.0	34.0	41.0
16	38.08658875845459	40.0	38.0	41.0	33.0	41.0
17	38.11874219288185	40.0	38.0	41.0	33.0	41.0
18	38.124381817188784	40.0	38.0	41.0	33.0	41.0
19	38.17449536726269	40.0	38.0	41.0	34.0	41.0
20	38.13295665364673	40.0	38.0	41.0	34.0	41.0
21	38.096913094111784	40.0	38.0	41.0	34.0	41.0
22	38.063658514669896	40.0	38.0	41.0	34.0	41.0
23	38.01470777373144	40.0	37.0	41.0	33.0	41.0
24	37.93356483806121	39.0	37.0	41.0	33.0	41.0
25	37.841695869032456	39.0	37.0	41.0	33.0	41.0
26	37.714109279393085	39.0	37.0	40.0	33.0	41.0
27	37.62417480192603	39.0	37.0	40.0	33.0	41.0
28	37.52748017498516	39.0	37.0	40.0	32.0	41.0
29	37.38116178727288	39.0	37.0	40.0	32.0	41.0
30	37.30990801475006	39.0	36.0	40.0	32.0	41.0
31	37.19738685074287	39.0	36.0	40.0	31.0	41.0
32	37.09819570782482	39.0	36.0	40.0	31.0	41.0
33	36.94021575394959	39.0	36.0	40.0	31.0	41.0
34	36.92166190091104	39.0	36.0	40.0	31.0	41.0
35	36.876932509500676	39.0	36.0	40.0	31.0	41.0
36	36.85895653736581	39.0	36.0	40.0	31.0	41.0
37	36.82267336867568	39.0	36.0	40.0	31.0	41.0
38	36.731473895815824	39.0	35.0	40.0	31.0	41.0
39	36.551846311868935	38.0	35.0	40.0	30.0	41.0
40	36.53229878556919	38.0	35.0	40.0	30.0	41.0
41	36.50980371429427	38.0	35.0	40.0	30.0	41.0
42	36.40695676992764	38.0	35.0	40.0	30.0	41.0
43	35.43946609442362	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	2.0
16	9.0
17	23.0
18	34.0
19	67.0
20	118.0
21	230.0
22	376.0
23	625.0
24	964.0
25	1499.0
26	2047.0
27	3090.0
28	4338.0
29	6079.0
30	8132.0
31	11237.0
32	15170.0
33	21005.0
34	30333.0
35	44532.0
36	69208.0
37	110613.0
38	157349.0
39	80510.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.154489764636864	17.611449089220933	12.137436992482263	27.096624153659942
2	20.91329848429591	19.82043408017393	31.95099992776489	27.315267507765274
3	21.678990681670427	20.711392534412983	28.252914510624727	29.356702273291862
4	16.623942240098945	15.230685475985348	31.50860390668633	36.63676837722938
5	17.334841461545373	33.627735464445344	32.16471015220467	16.87271292180461
6	37.31665935506377	32.07644236783177	14.558194192649285	16.048704084455178
7	31.470724518182987	27.99251573756455	19.647422175474947	20.889337568777517
8	28.64597923504777	31.1965834553402	18.99360631158704	21.163830998024984
9	27.58782292178699	13.354510554254736	18.128194421687446	40.92947210227082
10	18.487960520868018	25.17217503448786	31.38615658105925	24.95370786358487
11	38.24884467865065	19.951514382715725	19.55985912391141	22.23978181472222
12	23.474473696728808	23.47940682639436	27.051697437062955	25.99442203981388
13	32.62631014233841	17.918888777306194	22.46529631371886	26.98950476663654
14	24.642215961845764	19.151114094480004	22.551449899663666	33.65522004401056
15	27.644553912940832	25.27964678791595	20.08788018132775	26.98791911781547
16	27.278092852071296	24.018703608760532	21.98026395767375	26.72293958149442
17	26.070357000022902	24.167930781143465	23.321546677096713	26.44016554173692
18	26.074937763283774	23.122283475248903	24.048302386753843	26.75447637471348
19	26.91127942479708	23.457560109304058	23.846396436870915	25.784764029027947
20	27.274393004822134	22.966889890784035	23.52803339024051	26.230683714153326
21	27.430138955691685	23.09849874293285	22.933943631946242	26.53741866942922
22	27.69018536234718	23.01040714176229	22.703496003284055	26.595911492606472
23	26.927664462614803	22.97851798213855	22.950152486561628	27.143665068685017
24	26.821249808400765	23.41879980478901	23.02961111081747	26.730339275992748
25	27.072310871736864	22.883555236076685	23.06713813291613	26.976995759270316
26	26.90035606625193	23.323484692322467	23.0370108053158	26.739148436109804
27	26.899122783835544	22.931300883911128	22.94627645611012	27.22329987614321
28	26.444922488200127	23.23926912160341	23.440117972272287	26.875690417924176
29	26.24107852309145	23.303752173660257	23.80164590347627	26.653523399772016
30	26.33797928437907	23.747381477155205	23.475178429538172	26.439460808927556
31	26.91427453923688	23.259177823467954	23.032253858852588	26.794293778442572
32	26.16813867732223	23.238212022389362	23.640966822941166	26.952682477347246
33	26.006050131168397	23.210375076419464	23.93484040444616	26.848734387965983
34	26.76064278679542	22.7452514222389	23.61013476253147	26.883971028434207
35	26.33163668909479	22.783483177146923	24.364198868551473	26.520681265206814
36	25.95002387282392	22.993669737539886	24.120008950106676	26.936297439529522
37	26.149463257874068	22.173008381034936	24.609093519805636	27.06843484128536
38	25.431340525131652	22.307612347623554	25.405089227982824	26.855957899261966
39	25.340606175925974	22.014972048534947	25.814538990223596	26.829882785315483
40	24.905786032548086	21.86433541053329	26.596440042213494	26.633438514705134
41	24.445947874437756	21.673176635993173	27.092748123208438	26.788127366360637
42	24.609093519805636	21.74558793215537	26.831820800541234	26.813497747497756
43	23.702983310165244	21.557776638459735	27.424853459621453	27.31438659175357
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	8.0
2	14.0
3	40.0
4	66.0
5	66.0
6	89.0
7	112.0
8	112.0
9	112.0
10	157.0
11	202.0
12	202.0
13	368.5
14	535.0
15	791.5
16	1048.0
17	1069.0
18	1090.0
19	1090.0
20	1389.5
21	1689.0
22	1754.0
23	1819.0
24	2283.5
25	2748.0
26	2748.0
27	3356.5
28	3965.0
29	5057.5
30	6150.0
31	7600.0
32	9050.0
33	9050.0
34	10795.5
35	12541.0
36	14755.5
37	16970.0
38	19236.0
39	21502.0
40	21502.0
41	23613.5
42	25725.0
43	27633.5
44	29542.0
45	32016.0
46	34490.0
47	34490.0
48	36842.0
49	39194.0
50	41536.0
51	43878.0
52	46302.0
53	48726.0
54	48726.0
55	47924.5
56	47123.0
57	46107.5
58	45092.0
59	42689.5
60	40287.0
61	40287.0
62	38136.0
63	35985.0
64	32297.5
65	28610.0
66	25039.0
67	21468.0
68	21468.0
69	18649.0
70	15830.0
71	13854.5
72	11879.0
73	9777.0
74	7675.0
75	7675.0
76	6333.5
77	4992.0
78	4168.0
79	3344.0
80	2641.5
81	1939.0
82	1939.0
83	1523.0
84	1107.0
85	851.5
86	596.0
87	443.0
88	290.0
89	290.0
90	221.0
91	152.0
92	95.5
93	39.0
94	24.5
95	10.0
96	10.0
97	5.0
98	0.0
99	1.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	567591.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.22826101331373
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.91397226841478	67.64071774552158
2	6.014592842262472	9.049347204530592
3	1.5652312677768887	3.5324887907555924
4	0.7185016913812441	2.1620653111094246
5	0.414564024718705	1.5593465329134293
6	0.262459210053807	1.184660997556556
7	0.18608795192440744	0.979935111316161
8	0.13355423429618465	0.8037642237653307
9	0.10819362987387549	0.7325296765316786
>10	0.6273976149189058	8.818589815940983
>50	0.04487308755159598	2.28205962569222
>100	0.010337239564354797	1.1631207890249955
>500	2.3493726282419207E-4	0.09137417534147266
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	1.761832023411224E-4	0.0	0.0	0.0
6	0.0	1.761832023411224E-4	0.0	0.0	0.0
7	0.0	1.761832023411224E-4	0.0	0.0	0.0
8	0.0	1.761832023411224E-4	0.0	0.0	1.761832023411224E-4
9	0.0	1.761832023411224E-4	0.0	0.0	1.761832023411224E-4
10	0.0	1.761832023411224E-4	0.0	3.523664046822448E-4	1.761832023411224E-4
11	0.0	1.761832023411224E-4	0.0	3.523664046822448E-4	1.761832023411224E-4
12	0.0	1.761832023411224E-4	0.0	5.285496070233671E-4	1.761832023411224E-4
13	0.0	1.761832023411224E-4	0.0	5.285496070233671E-4	1.761832023411224E-4
14	0.0	1.761832023411224E-4	0.0	5.285496070233671E-4	1.761832023411224E-4
15	0.0	1.761832023411224E-4	0.0	7.047328093644896E-4	1.761832023411224E-4
16	0.0	1.761832023411224E-4	0.0	8.80916011705612E-4	1.761832023411224E-4
17	0.0	1.761832023411224E-4	0.0	0.0012332824163878567	1.761832023411224E-4
18	0.0	1.761832023411224E-4	0.0	0.0014094656187289792	1.761832023411224E-4
19	0.0	1.761832023411224E-4	0.0	0.001761832023411224	1.761832023411224E-4
20	0.0	1.761832023411224E-4	0.0	0.0019380152257523463	1.761832023411224E-4
21	0.0	1.761832023411224E-4	0.0	0.0029951144397990805	1.761832023411224E-4
22	0.0	1.761832023411224E-4	0.0	0.0036998472491635704	1.761832023411224E-4
23	0.0	1.761832023411224E-4	0.0	0.005461679272574794	1.761832023411224E-4
24	0.0	1.761832023411224E-4	0.0	0.007575877700668263	1.761832023411224E-4
25	0.0	1.761832023411224E-4	0.0	0.00880916011705612	1.761832023411224E-4
26	0.0	1.761832023411224E-4	0.0	0.011451908152172955	1.761832023411224E-4
27	0.0	1.761832023411224E-4	0.0	0.0236085491137104	1.761832023411224E-4
28	0.0	1.761832023411224E-4	0.0	0.07505404419731813	1.761832023411224E-4
29	0.0	1.761832023411224E-4	0.0	0.14499877552674373	1.761832023411224E-4
30	0.0	1.761832023411224E-4	0.0	0.23908060557690308	1.761832023411224E-4
31	0.0	1.761832023411224E-4	0.0	0.5003602946487876	1.761832023411224E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	500	0.0	22.57	1
CGTTTAT	50	2.699767E-4	22.2	31
TCGTTTA	55	5.138685E-4	20.181818	30
GTGCTAC	55	5.138685E-4	20.181818	1
CGGTGAT	70	1.2178183E-4	18.5	14
GTATCAA	615	0.0	17.747969	2
GTAGAAC	75	2.064944E-4	17.266666	3
CGGACCA	75	2.064944E-4	17.266666	9
GTATTAG	65	0.0015786019	17.076923	1
GTCCTAA	110	7.790477E-7	16.818182	1
TCGGTGA	80	3.3787914E-4	16.1875	13
AGTCGGT	80	3.3787914E-4	16.1875	11
TCTTATA	545	0.0	15.954128	37
CGCCTTA	70	0.002590327	15.857142	25
CTTATAC	310	0.0	15.516129	37
TGCACCG	110	1.4492152E-5	15.136364	5
AAGACGG	75	0.0041010026	14.8	5
GTCGGTG	100	1.0918152E-4	14.8	12
TAGAACT	180	3.3160177E-9	14.388889	4
GACGGAC	90	8.264917E-4	14.388889	7
>>END_MODULE