FastQCFastQC Report
Fri 10 Feb 2017
ERR1632276.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632276.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250923
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGTT250.005488258329.65
CTTATTC250.005488258329.63
TGGTAAT250.005488258329.624
AGTACTG405.921921E-527.755
GGTATCA1600.025.43751
ACAGTTC400.001927005123.1253
GTGTAAG400.001927005123.1259
AAGGTTC450.00381760320.5555555
TATAGAA450.00381760320.5555552
ACTGCAT555.1277084E-420.1818188
GATCGTC500.007019937518.57
AGTAGTG500.007019937518.55
CAGTACT500.007019937518.54
GTATCAA2300.017.6956522
TCTTATA2750.017.4909137
ATCTAAG1251.6424747E-716.27999926
CGATTTG700.002584927515.85714328
CATCTAA1302.5652662E-715.65384625
TCTAAGG1302.5652662E-715.65384627
CTAAGGG1101.4428157E-515.13636428
Produced by FastQC (version 0.11.5)