##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632276.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	250923
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.83918174101218	34.0	31.0	34.0	31.0	34.0
2	33.00594206190744	34.0	33.0	34.0	31.0	34.0
3	32.99918700159013	34.0	33.0	34.0	31.0	34.0
4	36.43048664331289	37.0	37.0	37.0	35.0	37.0
5	36.346552528066375	37.0	37.0	37.0	35.0	37.0
6	36.38226866409217	37.0	37.0	37.0	35.0	37.0
7	36.37291519709233	37.0	37.0	37.0	35.0	37.0
8	36.36331065705416	37.0	37.0	37.0	35.0	37.0
9	38.16676430618158	39.0	39.0	39.0	37.0	39.0
10	38.1046456482666	39.0	38.0	39.0	37.0	39.0
11	38.20173519366499	39.0	39.0	39.0	37.0	39.0
12	38.17628116992065	39.0	39.0	39.0	37.0	39.0
13	38.20177504652822	39.0	39.0	39.0	37.0	39.0
14	39.63406702454538	41.0	39.0	41.0	37.0	41.0
15	39.653658692108735	41.0	39.0	41.0	37.0	41.0
16	39.53509243871626	41.0	39.0	41.0	37.0	41.0
17	39.57952838121655	41.0	39.0	41.0	37.0	41.0
18	39.60281440920123	41.0	39.0	41.0	37.0	41.0
19	39.646130486244786	41.0	39.0	41.0	37.0	41.0
20	39.623095531298446	41.0	39.0	41.0	37.0	41.0
21	39.59863384384851	41.0	39.0	41.0	37.0	41.0
22	39.58504800277376	41.0	39.0	41.0	37.0	41.0
23	39.537276375621204	41.0	39.0	41.0	37.0	41.0
24	39.48624478425653	41.0	39.0	41.0	37.0	41.0
25	39.42305009903436	41.0	39.0	41.0	37.0	41.0
26	39.35629256783954	41.0	39.0	41.0	36.0	41.0
27	39.298019711226154	40.0	39.0	41.0	36.0	41.0
28	39.21946573251555	40.0	39.0	41.0	36.0	41.0
29	39.08445618775481	40.0	39.0	41.0	36.0	41.0
30	39.051816692770295	40.0	39.0	41.0	35.0	41.0
31	38.98908430076159	40.0	39.0	41.0	35.0	41.0
32	38.92921732961905	40.0	38.0	41.0	35.0	41.0
33	38.775134204516924	40.0	38.0	41.0	35.0	41.0
34	38.77527767482455	40.0	38.0	41.0	35.0	41.0
35	38.749357372580434	40.0	38.0	41.0	35.0	41.0
36	38.72124516285873	40.0	38.0	41.0	35.0	41.0
37	38.69122400098835	40.0	38.0	41.0	35.0	41.0
38	38.61684261705782	40.0	38.0	41.0	35.0	41.0
39	38.43770798212998	40.0	38.0	41.0	35.0	41.0
40	38.43155470004743	40.0	38.0	41.0	34.0	41.0
41	38.42956205688598	40.0	38.0	41.0	35.0	41.0
42	38.28931983118327	40.0	38.0	41.0	34.0	41.0
43	37.44993484056862	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	0.0
12	1.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	2.0
19	10.0
20	9.0
21	19.0
22	42.0
23	81.0
24	135.0
25	199.0
26	295.0
27	461.0
28	665.0
29	902.0
30	1286.0
31	1791.0
32	2434.0
33	3545.0
34	5377.0
35	8293.0
36	13958.0
37	26075.0
38	61580.0
39	123762.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.553201579767496	17.544824507916772	11.755399066645943	27.146574845669786
2	20.84384452600997	19.40077234848938	31.859972979758734	27.895410145741923
3	21.551232848323988	20.669288985067134	28.046851026011964	29.732627140596918
4	16.008098101808123	14.934461966419976	31.845625948996304	37.211813982775595
5	17.664383097603647	33.331739218804174	31.997465357898637	17.00641232569354
6	37.480422280938775	32.091916643751276	14.452242321349576	15.975418753960376
7	31.236674198857816	27.565029909573852	19.952335975578166	21.245959915990163
8	28.691271824424224	30.320456873224057	19.02137309054969	21.96689821180203
9	26.894704750062772	13.306073974884725	18.717295744112736	41.08192553093977
10	18.779067682117624	24.605556286191383	31.438728215428636	25.17664781626236
11	38.69354343762828	19.719196725688757	19.6984732368097	21.888786599873267
12	23.387652785914405	23.48768347261909	26.998322194458062	26.126341547008447
13	32.51595110850739	17.944947254735517	22.178915444180085	27.360186192577007
14	24.471252137109794	19.293568146403477	22.558713230752065	33.67646648573467
15	27.567421081367595	25.536917699852147	20.166345851117672	26.729315367662586
16	27.421559601949603	23.713649207127286	22.00196873144351	26.862822459479602
17	26.03149173252352	24.282349565404527	22.99589914037374	26.69025956169821
18	26.446360038736984	22.944488946808384	23.877444474998306	26.73170653945633
19	26.878365076138895	23.38047927053319	23.61162587726115	26.12952977606676
20	26.871191560757683	23.18201201165298	23.328272019703256	26.618524407886085
21	27.45025366347445	23.20632225822264	23.066040179656706	26.2773838986462
22	27.660676781323357	22.74602168792817	22.87913025111289	26.714171279635586
23	26.814600494972563	22.836089158825615	23.237008962908938	27.11230138329288
24	26.619719993782955	23.444642380331814	23.08237985358058	26.85325777230465
25	26.98995309317998	22.963618321158282	22.99350796858	27.052920617081732
26	26.915826767574114	23.512790776453336	23.06404753649526	26.507334919477287
27	26.914631181677244	23.05408432068802	23.000282955328927	27.031001542305805
28	26.292527986673203	23.071619580508763	23.770240272912407	26.865612159905627
29	26.21640901790589	23.225850161204832	23.841178369459954	26.716562451429322
30	25.967727151357188	23.67459340116291	23.804115206657023	26.55356424082288
31	26.887531234681557	23.20711931548722	23.17085320994887	26.734496239882354
32	26.018738816290256	23.17125173858116	23.346604336788577	27.463405108340012
33	25.742558474113576	23.10947980057627	23.867879787823355	27.2800819374868
34	26.624103808738138	22.69580708025968	23.72919182378658	26.950897287215604
35	26.290933872144045	22.733268771694902	24.32499212905951	26.650805227101543
36	25.781614280077953	22.981153580979026	24.1647836188791	27.072448520063926
37	25.971712437680083	21.841361692630805	24.959449711664536	27.227476158024572
38	25.348413656779172	21.70068108543258	25.980480067590456	26.970425190197787
39	25.09773914706902	21.483482980834758	26.291332400776334	27.127445471319888
40	24.708775201954385	21.516959385947082	26.99632955129661	26.77793586080192
41	24.147646887690648	21.727781032428275	27.331492131052155	26.793079948828925
42	24.303870111548164	21.785966212742554	27.460216879281692	26.449946796427586
43	23.432686521363127	21.30892743989192	27.883055758140944	27.37533028060401
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	7.5
2	9.0
3	22.5
4	36.0
5	36.0
6	36.5
7	37.0
8	39.5
9	42.0
10	62.0
11	82.0
12	82.0
13	157.5
14	233.0
15	320.5
16	408.0
17	395.5
18	383.0
19	383.0
20	516.0
21	649.0
22	691.0
23	733.0
24	945.5
25	1158.0
26	1158.0
27	1452.0
28	1746.0
29	2214.0
30	2682.0
31	3336.5
32	3991.0
33	3991.0
34	4817.0
35	5643.0
36	6603.0
37	7563.0
38	8595.0
39	9627.0
40	9627.0
41	10720.0
42	11813.0
43	12627.0
44	13441.0
45	14411.5
46	15382.0
47	15382.0
48	16497.5
49	17613.0
50	18330.0
51	19047.0
52	19532.5
53	20018.0
54	20018.0
55	19996.0
56	19974.0
57	19794.0
58	19614.0
59	19101.0
60	18588.0
61	18588.0
62	17240.0
63	15892.0
64	14561.0
65	13230.0
66	11791.5
67	10353.0
68	10353.0
69	8887.0
70	7421.0
71	6436.0
72	5451.0
73	4288.0
74	3125.0
75	3125.0
76	2545.5
77	1966.0
78	1614.0
79	1262.0
80	1002.0
81	742.0
82	742.0
83	591.5
84	441.0
85	360.5
86	280.0
87	212.0
88	144.0
89	144.0
90	115.5
91	87.0
92	48.5
93	10.0
94	5.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	250923.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.06788845134857
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.64608339716426	73.37908382447137
2	5.0564839755648	8.097239898231077
3	1.3469860919287182	3.2355099646219974
4	0.5850818350251711	1.873850684068229
5	0.33884754509228493	1.3565403721231184
6	0.21121308869875352	1.0146831615237952
7	0.14894107392116504	0.8347778104780731
8	0.11046941569893007	0.7076042282774069
9	0.07159900795668382	0.5159503244072681
>10	0.4473620493931448	6.641450768034579
>50	0.03244072663711267	1.8489922536377816
>100	0.004491792918984829	0.4943167101253371
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	7.970572645791737E-4	0.0
18	0.0	0.0	0.0	7.970572645791737E-4	0.0
19	0.0	0.0	0.0	0.0011955858968687606	0.0
20	0.0	0.0	0.0	0.0011955858968687606	0.0
21	0.0	0.0	0.0	0.0015941145291583473	0.0
22	0.0	0.0	0.0	0.002789700426027108	0.0
23	0.0	0.0	0.0	0.004782343587475042	0.0
24	0.0	0.0	0.0	0.006774986748922977	0.0
25	0.0	0.0	0.0	0.00757204401350215	0.0
26	0.0	0.0	0.0	0.011557330336398018	0.0
27	0.0	0.0	0.0	0.03825874869980034	0.0
28	0.0	0.0	0.0	0.11676888926084894	0.0
29	0.0	0.0	0.0	0.1936849152927392	0.0
30	0.0	0.0	0.0	0.2973023596880318	0.0
31	0.0	0.0	0.0	0.5858370894656927	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGGTT	25	0.0054882583	29.6	5
CTTATTC	25	0.0054882583	29.6	3
TGGTAAT	25	0.0054882583	29.6	24
AGTACTG	40	5.921921E-5	27.75	5
GGTATCA	160	0.0	25.4375	1
ACAGTTC	40	0.0019270051	23.125	3
GTGTAAG	40	0.0019270051	23.125	9
AAGGTTC	45	0.003817603	20.555555	5
TATAGAA	45	0.003817603	20.555555	2
ACTGCAT	55	5.1277084E-4	20.181818	8
GATCGTC	50	0.0070199375	18.5	7
AGTAGTG	50	0.0070199375	18.5	5
CAGTACT	50	0.0070199375	18.5	4
GTATCAA	230	0.0	17.695652	2
TCTTATA	275	0.0	17.49091	37
ATCTAAG	125	1.6424747E-7	16.279999	26
CGATTTG	70	0.0025849275	15.857143	28
CATCTAA	130	2.5652662E-7	15.653846	25
TCTAAGG	130	2.5652662E-7	15.653846	27
CTAAGGG	110	1.4428157E-5	15.136364	28
>>END_MODULE