FastQCFastQC Report
Fri 10 Feb 2017
ERR1632275.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632275.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67431
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20963.108362622532663No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18572.753926235707612No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13872.056917441532826No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6430.9535673503284838No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT4230.6273079147573074TruSeq Adapter, Index 11 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGT2390.3544363868250508No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2160.3203274458335187No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1980.29363349201405886No Hit
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC1710.25359256128486896TruSeq Adapter, Index 11 (95% over 21bp)
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA1580.23431359463748128No Hit
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCC1500.22244961516216577No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1340.1987216562115348No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATG1300.192789666473877No Hit
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTC1230.18240868443297592RNA PCR Primer, Index 11 (95% over 24bp)
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1200.17795969212973262No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1100.1631297177855882No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC990.14681674600702943No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT920.13643576396612833No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT870.12902077679405613No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA830.12308878705639839No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA830.12308878705639839No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC810.12012279218756952No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG810.12012279218756952No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT800.11863979475315507No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT780.11567379988432619No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA730.10825881271225402No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA730.10825881271225402No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA710.10529281784342513No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA690.10232682297459626No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA680.10084382554018181No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT200.001829671437.06
GACCGTT200.001829671437.07
CCGTCTT850.030.47058937
TAGGACC250.005460164529.64
TCCTAAG250.005460164529.62
AGGACCG250.005460164529.65
ACCGTTA250.005460164529.68
GTCCTAA250.005460164529.61
TTAGGAC250.005460164529.63
GAGCTGA405.8588328E-527.756
GCCGTCT950.027.26315936
TTTTTGA358.7827013E-426.4285721
CTAAGGT358.7827013E-426.4285724
TGGGGGA358.7827013E-426.4285726
TGAGCTG358.7827013E-426.4285725
TATGCCG1000.025.90000233
GCCTTAT802.6306225E-823.12526
CGCCTTA802.6306225E-823.12525
CAACCTG400.001912490223.12534
CTCGCCT802.6306225E-823.12523
Produced by FastQC (version 0.11.5)