##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632275.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	67431
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.7124319674927	34.0	31.0	34.0	31.0	34.0
2	32.80153045335231	34.0	31.0	34.0	31.0	34.0
3	32.72201213091901	34.0	31.0	34.0	31.0	34.0
4	36.251486704928	37.0	35.0	37.0	35.0	37.0
5	36.19427266390829	37.0	35.0	37.0	35.0	37.0
6	36.30324331538907	37.0	37.0	37.0	35.0	37.0
7	36.27605997241625	37.0	36.0	37.0	35.0	37.0
8	36.31227476976465	37.0	37.0	37.0	35.0	37.0
9	38.08482745324851	39.0	39.0	39.0	37.0	39.0
10	38.00111224807581	39.0	38.0	39.0	35.0	39.0
11	38.092272100369264	39.0	38.0	39.0	37.0	39.0
12	38.084100784505644	39.0	38.0	39.0	37.0	39.0
13	38.13489344663434	39.0	39.0	39.0	37.0	39.0
14	39.34245376755498	40.0	39.0	41.0	37.0	41.0
15	39.36069463599828	40.0	39.0	41.0	37.0	41.0
16	39.17404457890288	40.0	39.0	41.0	36.0	41.0
17	39.303391615132504	40.0	39.0	41.0	37.0	41.0
18	39.49035310168913	40.0	39.0	41.0	37.0	41.0
19	39.55952010203023	41.0	39.0	41.0	37.0	41.0
20	39.59380700271389	41.0	39.0	41.0	37.0	41.0
21	39.56822529697023	41.0	39.0	41.0	37.0	41.0
22	39.489137043792915	41.0	39.0	41.0	37.0	41.0
23	39.429016327801754	41.0	39.0	41.0	36.0	41.0
24	39.344574453886196	41.0	39.0	41.0	36.0	41.0
25	39.24878764959737	40.0	39.0	41.0	35.0	41.0
26	39.09532707508416	40.0	38.0	41.0	35.0	41.0
27	38.9455443342083	40.0	38.0	41.0	35.0	41.0
28	38.784698432471714	40.0	38.0	41.0	35.0	41.0
29	38.519686790941854	40.0	38.0	41.0	35.0	41.0
30	38.286974833533534	40.0	37.0	41.0	35.0	41.0
31	37.97628687102371	40.0	36.0	41.0	35.0	41.0
32	37.72021770402337	39.0	35.0	41.0	34.0	41.0
33	37.423336447627946	39.0	35.0	41.0	33.0	41.0
34	37.26559001052928	39.0	35.0	41.0	33.0	41.0
35	37.072830004004096	39.0	35.0	41.0	33.0	41.0
36	36.90221114917471	39.0	35.0	41.0	33.0	41.0
37	36.702362414913026	39.0	35.0	41.0	33.0	41.0
38	36.479453070546185	39.0	35.0	41.0	33.0	41.0
39	36.07932553276683	39.0	35.0	41.0	31.0	41.0
40	35.82663759991695	38.0	35.0	41.0	30.0	41.0
41	35.53270750841601	38.0	35.0	41.0	28.0	41.0
42	35.091871691061975	38.0	35.0	41.0	24.0	41.0
43	34.14539306846999	37.0	34.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	1.0
16	0.0
17	0.0
18	1.0
19	5.0
20	3.0
21	7.0
22	19.0
23	33.0
24	75.0
25	95.0
26	160.0
27	278.0
28	407.0
29	597.0
30	800.0
31	1065.0
32	1255.0
33	1699.0
34	2258.0
35	3455.0
36	5338.0
37	12791.0
38	15674.0
39	21414.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.79792676958669	16.80977591908766	16.80236093191559	20.589936379410066
2	19.439130370304458	23.15552194094704	33.303673384645045	24.101674304103454
3	20.71895715620412	24.669662321484186	33.411932197357295	21.1994483249544
4	13.403330812237696	18.75695155047382	30.425175364446623	37.414542272841864
5	12.75377793596417	39.1941391941392	30.856727617861225	17.195355252035412
6	28.39346887929884	33.11978170277766	18.178582551052187	20.30816686687132
7	24.91880589046581	35.044712372647595	18.961605196423008	21.074876540463585
8	33.69370170989604	29.538342898666787	17.813765182186234	18.95419020925094
9	28.820572140410196	11.60445492429298	18.60420281472913	40.97077012056769
10	21.275081194109536	28.79387818659074	24.482804644747965	25.448235974551764
11	32.28337114976791	24.657798342008867	16.77566697809613	26.28316353012709
12	21.458972875976926	33.45493912295532	24.97367679553914	20.112411205528613
13	35.21377408017084	19.11880292447094	25.034479690350135	20.632943305008084
14	23.240052794708667	23.693850009639483	27.77505894914802	25.291038246503835
15	30.173065800596166	26.106686835431773	23.26971274339695	20.450534620575105
16	21.800062285892245	25.250997315774644	28.27927807684893	24.669662321484186
17	18.203793507437233	32.08316649612196	26.722130770713765	22.99090922572704
18	19.563702154795273	22.74473165161424	31.974907683409707	25.71665851018078
19	21.027420622562325	28.156189289792525	33.49498005368451	17.321410033960642
20	23.493645355993532	22.92862333348163	34.62057510640506	18.957156204119766
21	26.86004953211431	22.71655470036037	31.53000845308538	18.89338731443995
22	25.010751731399505	24.166926191217687	30.58385608992896	20.23846598745384
23	21.528673755394404	25.36073912592131	31.26455191232519	21.846035206359094
24	20.069404279930595	26.647980898993044	33.53650398184811	19.746110839228248
25	19.000163129717784	27.278254808619177	31.473654550577628	22.247927511085404
26	21.539054737435304	25.024098708309232	31.84885290148448	21.587993652770983
27	20.45201761800952	26.664293870771605	31.24378994824339	21.639898562975485
28	18.91711527339058	26.49226616837953	33.367442274324866	21.22317628390503
29	20.235499992585012	26.42256528896205	33.9561922557874	19.38574246266554
30	19.095074965520308	27.428037549495038	34.774806839584166	18.702080645400486
31	18.96457119129184	28.446856786937758	32.642256528896205	19.9463154928742
32	18.50039299432012	28.607020509854518	34.33732259643191	18.555263899393452
33	18.097017692159394	27.506636413519004	34.767391852412096	19.62895404190951
34	19.072830004004093	28.64854443801812	32.1587993652771	20.11982619270069
35	17.656567454138305	29.154246563153446	33.050080823360176	20.139105159348077
36	17.524580682475417	28.865062063442632	31.687206181133305	21.92315107294864
37	17.23984517506785	29.170559534932007	32.195874301137465	21.39372098886269
38	17.834527146268037	26.75030772196764	33.671456748379825	21.743708383384497
39	18.75695155047382	26.44925924278151	34.01402915572956	20.77976005101511
40	17.486022749180645	26.200115673799885	34.47524135783245	21.83862021918702
41	16.180985006895938	26.831872580860434	34.91717459328795	22.069967818955675
42	16.317420770862068	26.2223606353161	35.304236923670125	22.15598167015171
43	15.346057451320611	26.98165532173629	35.62308137206923	22.049205854873872
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4.0
1	10.5
2	17.0
3	63.5
4	110.0
5	110.0
6	200.5
7	291.0
8	284.5
9	278.0
10	476.0
11	674.0
12	674.0
13	1230.0
14	1786.0
15	2850.0
16	3914.0
17	3398.5
18	2883.0
19	2883.0
20	3091.0
21	3299.0
22	2237.0
23	1175.0
24	1053.5
25	932.0
26	932.0
27	997.5
28	1063.0
29	1124.5
30	1186.0
31	1249.0
32	1312.0
33	1312.0
34	1226.5
35	1141.0
36	1153.0
37	1165.0
38	1411.0
39	1657.0
40	1657.0
41	1900.0
42	2143.0
43	2572.0
44	3001.0
45	3635.0
46	4269.0
47	4269.0
48	5444.5
49	6620.0
50	6087.5
51	5555.0
52	5150.0
53	4745.0
54	4745.0
55	4056.5
56	3368.0
57	3497.5
58	3627.0
59	3209.0
60	2791.0
61	2791.0
62	2408.5
63	2026.0
64	1861.5
65	1697.0
66	1556.5
67	1416.0
68	1416.0
69	1271.0
70	1126.0
71	981.0
72	836.0
73	714.5
74	593.0
75	593.0
76	489.5
77	386.0
78	289.5
79	193.0
80	142.0
81	91.0
82	91.0
83	66.0
84	41.0
85	27.0
86	13.0
87	9.0
88	5.0
89	5.0
90	3.5
91	2.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	67431.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.00115673799884
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.18666283964791	54.0567394818407
2	7.335916570991198	9.096706262698165
3	1.954171450440107	3.634826711749789
4	0.9950248756218906	2.4677077308656257
5	0.5644852659778034	1.7499369726090372
6	0.37791810179869884	1.4058815678248875
7	0.24875621890547264	1.0796221322537112
8	0.19613471106008418	0.9728463169758715
9	0.13394565633371602	0.747430706944877
>10	0.9041331802525832	9.916803843929351
>50	0.0645809414466131	2.7776541946582434
>100	0.02870264064293915	3.22107042754816
>500	0.0023918867202449294	0.9535673503284838
>1k	0.007175660160734788	7.919206299773101
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2096	3.108362622532663	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1857	2.753926235707612	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1387	2.056917441532826	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	643	0.9535673503284838	No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT	423	0.6273079147573074	TruSeq Adapter, Index 11 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGT	239	0.3544363868250508	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	216	0.3203274458335187	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	198	0.29363349201405886	No Hit
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC	171	0.25359256128486896	TruSeq Adapter, Index 11 (95% over 21bp)
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	158	0.23431359463748128	No Hit
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCC	150	0.22244961516216577	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	134	0.1987216562115348	No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATG	130	0.192789666473877	No Hit
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTC	123	0.18240868443297592	RNA PCR Primer, Index 11 (95% over 24bp)
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	120	0.17795969212973262	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	110	0.1631297177855882	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	99	0.14681674600702943	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	92	0.13643576396612833	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	87	0.12902077679405613	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	83	0.12308878705639839	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	83	0.12308878705639839	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	81	0.12012279218756952	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	81	0.12012279218756952	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	80	0.11863979475315507	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	78	0.11567379988432619	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	73	0.10825881271225402	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	73	0.10825881271225402	No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	71	0.10529281784342513	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	69	0.10232682297459626	No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	68	0.10084382554018181	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0014829974344144385	0.0
13	0.0	0.0	0.0	0.0014829974344144385	0.0
14	0.0	0.0	0.0	0.002965994868828877	0.0
15	0.0	0.0	0.0	0.004448992303243316	0.0
16	0.0	0.0	0.0	0.005931989737657754	0.0
17	0.0	0.0	0.0	0.011863979475315508	0.0
18	0.0	0.0	0.0	0.011863979475315508	0.0
19	0.0	0.0	0.0	0.013346976909729946	0.0
20	0.0	0.0	0.0	0.013346976909729946	0.0
21	0.0	0.0	0.0	0.014829974344144384	0.0
22	0.0	0.0	0.0	0.017795969212973262	0.0
23	0.0	0.0	0.0	0.023727958950631015	0.0
24	0.0	0.0	0.0	0.040040930729189836	0.0
25	0.0	0.0	0.0	0.04745591790126203	0.0
26	0.0	0.0	0.0	0.06228589224540641	0.0
27	0.0	0.0	0.0	0.08156485889279412	0.0
28	0.0	0.0	0.0	0.14829974344144384	0.0
29	0.0	0.0	0.0	0.28621850484198663	0.0
30	0.0	0.0	0.0	0.5635390250774867	0.0
31	0.0	0.0	0.0	1.1359760347614598	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGACCGT	20	0.0018296714	37.0	6
GACCGTT	20	0.0018296714	37.0	7
CCGTCTT	85	0.0	30.470589	37
TAGGACC	25	0.0054601645	29.6	4
TCCTAAG	25	0.0054601645	29.6	2
AGGACCG	25	0.0054601645	29.6	5
ACCGTTA	25	0.0054601645	29.6	8
GTCCTAA	25	0.0054601645	29.6	1
TTAGGAC	25	0.0054601645	29.6	3
GAGCTGA	40	5.8588328E-5	27.75	6
GCCGTCT	95	0.0	27.263159	36
TTTTTGA	35	8.7827013E-4	26.428572	1
CTAAGGT	35	8.7827013E-4	26.428572	4
TGGGGGA	35	8.7827013E-4	26.428572	6
TGAGCTG	35	8.7827013E-4	26.428572	5
TATGCCG	100	0.0	25.900002	33
GCCTTAT	80	2.6306225E-8	23.125	26
CGCCTTA	80	2.6306225E-8	23.125	25
CAACCTG	40	0.0019124902	23.125	34
CTCGCCT	80	2.6306225E-8	23.125	23
>>END_MODULE