FastQCFastQC Report
Fri 10 Feb 2017
ERR1632274.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632274.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102711
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19841.9316334180370165No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16301.5869770521171052No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT11851.1537225808336011No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5660.5510607432504795No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT2710.2638471049838868TruSeq Adapter, Index 11 (95% over 21bp)
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2330.2268500939529359No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT2000.19472111068921535No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT1960.19082668847543108No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC1850.18011702738752422No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC1720.16746015519272522No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1550.15090886078414192No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA1470.1431200163565733No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC1410.13727838303589684No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTG1280.12462151084109782No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT1250.1217006941807596No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA1160.11293824419974492No Hit
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC1150.11196463864629883No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA1130.11001742753940669No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC1090.10612300532562237No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT705.456968E-1231.71428736
ATTAACC250.00547335529.629
CCGTCTT754.4929038E-1027.13333137
ATGCCGT809.531504E-1025.437534
CGTATGC851.9244908E-923.94117731
CTCGTAT851.9244908E-923.94117729
GGTATCA7000.023.2571431
CTTGATT400.001919301923.12533
TTTGCAG400.001919301923.12522
AGGGAGC400.001919301923.12510
GTATGCC903.7271093E-922.6111132
TGCCGTC903.7271093E-922.6111135
TCTCGTA956.9558155E-921.42105328
TCGTATG956.9558155E-921.42105330
TCTTGAT450.003802487420.55555532
GCTGATG450.003802487420.5555558
TATGCCG1001.2558303E-820.3533
GACCGTA1052.2000677E-819.3809516
GTACTAG1052.2000677E-819.3809520
CGTACTA1052.2000677E-819.3809519