##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632274.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	102711
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.20044591134348	33.0	31.0	34.0	30.0	34.0
2	32.326420733903866	34.0	31.0	34.0	31.0	34.0
3	32.26823806602993	34.0	31.0	34.0	30.0	34.0
4	35.952624353769316	37.0	35.0	37.0	35.0	37.0
5	35.76418299889983	37.0	35.0	37.0	35.0	37.0
6	35.817283445784774	37.0	35.0	37.0	35.0	37.0
7	35.823923435659275	37.0	35.0	37.0	35.0	37.0
8	35.816825851174656	37.0	35.0	37.0	35.0	37.0
9	37.47934495818364	39.0	37.0	39.0	35.0	39.0
10	37.31428960870793	39.0	37.0	39.0	34.0	39.0
11	37.47143928108966	39.0	37.0	39.0	35.0	39.0
12	37.410267644166645	39.0	37.0	39.0	35.0	39.0
13	37.475927602691044	39.0	37.0	39.0	35.0	39.0
14	38.56158541928323	40.0	38.0	41.0	35.0	41.0
15	38.57192511026083	40.0	38.0	41.0	35.0	41.0
16	38.42231114486277	40.0	38.0	41.0	34.0	41.0
17	38.549113532143586	40.0	38.0	41.0	35.0	41.0
18	38.64558810643456	40.0	38.0	41.0	35.0	41.0
19	38.695904041436656	40.0	38.0	41.0	35.0	41.0
20	38.68260458957658	40.0	38.0	41.0	35.0	41.0
21	38.65349378352854	40.0	38.0	41.0	35.0	41.0
22	38.60503743513353	40.0	38.0	41.0	35.0	41.0
23	38.5400200562744	40.0	38.0	41.0	35.0	41.0
24	38.44213375393093	40.0	38.0	41.0	34.0	41.0
25	38.35297095734634	40.0	38.0	41.0	34.0	41.0
26	38.167129129304556	40.0	37.0	41.0	34.0	41.0
27	38.01927738995823	40.0	37.0	41.0	34.0	41.0
28	37.9082084684211	40.0	37.0	41.0	33.0	41.0
29	37.67528307581466	39.0	37.0	41.0	33.0	41.0
30	37.51857152593198	39.0	36.0	41.0	33.0	41.0
31	37.26226986398731	39.0	35.0	40.0	33.0	41.0
32	37.07722639249934	39.0	35.0	40.0	32.0	41.0
33	36.850746268656714	39.0	35.0	40.0	32.0	41.0
34	36.745752645773095	39.0	35.0	40.0	31.0	41.0
35	36.59466853598933	39.0	35.0	40.0	31.0	41.0
36	36.468265326985424	39.0	35.0	40.0	31.0	41.0
37	36.33016911528463	38.0	35.0	40.0	31.0	41.0
38	36.156088442328475	38.0	35.0	40.0	30.0	41.0
39	35.838303589683676	38.0	35.0	40.0	29.0	41.0
40	35.67113551615699	38.0	35.0	40.0	28.0	41.0
41	35.50780344851087	38.0	35.0	40.0	28.0	41.0
42	35.195159233188264	38.0	35.0	40.0	26.0	41.0
43	34.22788211583959	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	2.0
13	0.0
14	0.0
15	2.0
16	2.0
17	4.0
18	6.0
19	7.0
20	19.0
21	28.0
22	84.0
23	103.0
24	199.0
25	289.0
26	408.0
27	666.0
28	934.0
29	1282.0
30	1626.0
31	1995.0
32	2643.0
33	3462.0
34	4786.0
35	6842.0
36	11016.0
37	19582.0
38	27645.0
39	19077.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.128778806554315	18.779877520421376	14.61187214611872	23.47947152690559
2	20.612203172006893	21.67440683081656	32.18837320248075	25.525016794695798
3	21.008460632259446	23.014088072358366	31.750250703430012	24.227200591952176
4	14.645948340489333	17.452853151074375	32.18350517471352	35.71769333372278
5	14.736493656959821	36.318407960199	31.649969331425066	17.295129051416108
6	31.22255649346224	34.524052925197886	16.003154481993164	18.25023609934671
7	26.937718452746058	32.60312916824877	19.79924253487942	20.65990984412575
8	30.812668555461443	30.13795990692331	18.400171354577406	20.649200183037845
9	28.37378664407902	12.171043023629407	20.167265434082037	39.28790489820954
10	19.809952195967327	27.30866216860901	28.101177089114117	24.78020854630955
11	35.89002151668273	21.83213093047483	18.531608104292626	23.746239448549815
12	22.883624928196593	29.334735325330296	25.92614228271558	21.855497463757533
13	33.73153800469278	18.467350137765187	24.113288742198986	23.68782311534305
14	23.696585565324067	21.836025352688612	26.221144765409743	28.246244316577584
15	28.640554565723242	26.754680608698195	22.05216578555364	22.552599040024923
16	24.396607958251792	24.57867219674621	25.234882339768866	25.78983750523313
17	21.99764387456066	28.093388244686544	26.03713331580843	23.871834564944358
18	22.537021351169788	22.61393618989203	29.070888220346408	25.778154238591778
19	23.46681465471079	25.30887636183077	30.483589878396668	20.740719105061775
20	25.151152262172506	22.438687190271732	30.50014117280525	21.910019374750515
21	26.52198888142458	22.63535551206784	29.034864814868904	21.807790791638677
22	25.632113405574863	24.39952877491213	27.71173486773569	22.256622951777317
23	23.063741955584113	23.403530293736797	28.885903165191657	24.646824585487437
24	22.61783061210581	25.095656745626076	29.712494280067375	22.574018362200736
25	22.590569656609322	24.510519808004986	28.890771192958887	24.00813934242681
26	23.742345026336032	24.600091518922024	28.89466561517267	22.762897839569277
27	22.922569150334436	25.214436623146497	28.780753765419476	23.08224046109959
28	22.080400346603575	24.39758156380524	30.15937922909912	23.362638860492062
29	21.79708113055077	25.481204544790724	30.532270156068968	22.189444168589535
30	21.641304241999396	25.48509896700451	30.91587074412672	21.95772604686937
31	22.268306218418672	26.46357254821782	28.885903165191657	22.38221806817186
32	20.832238027085705	26.11404815453067	29.77675224659481	23.276961571788807
33	20.867287827009765	25.231961523108527	30.863296044240634	23.03745460564107
34	21.68803730856481	25.73531559424015	29.757280135525892	22.81936696166915
35	22.04729775778641	26.535619359172824	29.88190164636699	21.535181236673772
36	21.800001947211108	26.24061687647866	28.661973887899055	23.297407288411172
37	20.643358549717167	26.209461498768388	29.645315496879594	23.50186445463485
38	21.390114009210308	24.6925840464994	30.343390678700434	23.57391126558986
39	21.321961620469082	24.922354957112674	30.376493267517596	23.379190154900645
40	20.42137648353146	25.02750435688485	31.076515660445324	23.474603499138357
41	20.311359055992057	24.706214524247645	30.97428707733349	24.00813934242681
42	19.767113551615697	25.14920505106561	31.789194925567855	23.294486471750837
43	19.03496217542425	24.411212041553483	31.952760658546797	24.601065124475472
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6.0
1	10.0
2	14.0
3	63.5
4	113.0
5	113.0
6	168.5
7	224.0
8	249.5
9	275.0
10	414.0
11	553.0
12	553.0
13	1123.5
14	1694.0
15	2643.5
16	3593.0
17	3095.5
18	2598.0
19	2598.0
20	2761.5
21	2925.0
22	2048.5
23	1172.0
24	1044.5
25	917.0
26	917.0
27	1042.0
28	1167.0
29	1330.5
30	1494.0
31	1778.5
32	2063.0
33	2063.0
34	2394.5
35	2726.0
36	2968.0
37	3210.0
38	3488.5
39	3767.0
40	3767.0
41	4177.5
42	4588.0
43	4881.0
44	5174.0
45	5600.5
46	6027.0
47	6027.0
48	6877.0
49	7727.0
50	7720.0
51	7713.0
52	7244.0
53	6775.0
54	6775.0
55	6821.0
56	6867.0
57	6860.0
58	6853.0
59	6424.5
60	5996.0
61	5996.0
62	5176.5
63	4357.0
64	3867.5
65	3378.0
66	3011.0
67	2644.0
68	2644.0
69	2205.0
70	1766.0
71	1577.0
72	1388.0
73	1211.5
74	1035.0
75	1035.0
76	849.0
77	663.0
78	582.5
79	502.0
80	404.0
81	306.0
82	306.0
83	253.0
84	200.0
85	161.5
86	123.0
87	104.0
88	85.0
89	85.0
90	55.5
91	26.0
92	15.5
93	5.0
94	3.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	102711.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.597969058815515
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.1125281903725	54.530673442961316
2	6.922777820981414	8.667036636776976
3	2.104362703165098	3.9518649414376257
4	0.9938564429582395	2.4885357946081723
5	0.6081343805894703	1.9033988569870801
6	0.3732794151955829	1.4019919969623507
7	0.31262151022630064	1.36986301369863
8	0.21463566373746013	1.074860531004469
9	0.1757523913212536	0.9901568478546601
>10	1.0591803406174665	12.233353779049956
>50	0.09176452290224746	3.723067636377798
>100	0.02488529434637219	2.4418027280427608
>500	0.0015553308966482619	0.5510607432504795
>1k	0.004665992689944785	4.6723330509877234
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1984	1.9316334180370165	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1630	1.5869770521171052	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1185	1.1537225808336011	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	566	0.5510607432504795	No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT	271	0.2638471049838868	TruSeq Adapter, Index 11 (95% over 21bp)
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	233	0.2268500939529359	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT	200	0.19472111068921535	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT	196	0.19082668847543108	No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC	185	0.18011702738752422	No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC	172	0.16746015519272522	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	155	0.15090886078414192	No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA	147	0.1431200163565733	No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC	141	0.13727838303589684	No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTG	128	0.12462151084109782	No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT	125	0.1217006941807596	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	116	0.11293824419974492	No Hit
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC	115	0.11196463864629883	No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA	113	0.11001742753940669	No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC	109	0.10612300532562237	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	9.736055534460768E-4	0.0
2	0.0	0.0	0.0	9.736055534460768E-4	0.0
3	0.0	0.0	0.0	9.736055534460768E-4	0.0
4	0.0	0.0	0.0	9.736055534460768E-4	0.0
5	0.0	0.0	0.0	9.736055534460768E-4	0.0
6	0.0	0.0	0.0	9.736055534460768E-4	0.0
7	0.0	0.0	0.0	9.736055534460768E-4	0.0
8	0.0	0.0	0.0	9.736055534460768E-4	0.0
9	0.0	0.0	0.0	9.736055534460768E-4	0.0
10	0.0	0.0	0.0	9.736055534460768E-4	0.0
11	0.0	0.0	0.0	9.736055534460768E-4	0.0
12	0.0	0.0	0.0	9.736055534460768E-4	0.0
13	0.0	0.0	0.0	9.736055534460768E-4	0.0
14	0.0	0.0	0.0	9.736055534460768E-4	9.736055534460768E-4
15	0.0	0.0	0.0	0.0019472111068921537	9.736055534460768E-4
16	0.0	0.0	0.0	0.0038944222137843073	9.736055534460768E-4
17	0.0	0.0	0.0	0.0038944222137843073	9.736055534460768E-4
18	0.0	0.0	0.0	0.0038944222137843073	9.736055534460768E-4
19	0.0	0.0	0.0	0.0038944222137843073	9.736055534460768E-4
20	0.0	0.0	0.0	0.004868027767230384	9.736055534460768E-4
21	0.0	0.0	0.0	0.005841633320676461	9.736055534460768E-4
22	0.0	0.0	0.0	0.007788844427568615	9.736055534460768E-4
23	0.0	0.0	0.0	0.013630477748245075	9.736055534460768E-4
24	0.0	0.0	0.0	0.019472111068921535	0.0019472111068921537
25	0.0	0.0	0.0	0.020445716622367615	0.0019472111068921537
26	0.0	0.0	0.0	0.026287349943044075	0.0019472111068921537
27	0.0	0.0	0.0	0.048680277672303846	0.0019472111068921537
28	0.0	0.0	0.0	0.1051493997721763	0.0029208166603382304
29	0.0	0.0	0.0	0.2307445161667202	0.0029208166603382304
30	0.0	0.0	0.0	0.4108615435542444	0.0029208166603382304
31	0.0	0.0	0.0	0.760385937241386	0.0029208166603382304
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGTCT	70	5.456968E-12	31.714287	36
ATTAACC	25	0.005473355	29.6	29
CCGTCTT	75	4.4929038E-10	27.133331	37
ATGCCGT	80	9.531504E-10	25.4375	34
CGTATGC	85	1.9244908E-9	23.941177	31
CTCGTAT	85	1.9244908E-9	23.941177	29
GGTATCA	700	0.0	23.257143	1
CTTGATT	40	0.0019193019	23.125	33
TTTGCAG	40	0.0019193019	23.125	22
AGGGAGC	40	0.0019193019	23.125	10
GTATGCC	90	3.7271093E-9	22.61111	32
TGCCGTC	90	3.7271093E-9	22.61111	35
TCTCGTA	95	6.9558155E-9	21.421053	28
TCGTATG	95	6.9558155E-9	21.421053	30
TCTTGAT	45	0.0038024874	20.555555	32
GCTGATG	45	0.0038024874	20.555555	8
TATGCCG	100	1.2558303E-8	20.35	33
GACCGTA	105	2.2000677E-8	19.38095	16
GTACTAG	105	2.2000677E-8	19.38095	20
CGTACTA	105	2.2000677E-8	19.38095	19
>>END_MODULE