FastQCFastQC Report
Fri 10 Feb 2017
ERR1632273.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632273.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1097347
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4750.029.2105261
TATTAGA1151.3460522E-1020.9130442
CGTTTCG555.142889E-420.18181823
TTAGAGT759.265146E-619.7333324
ATTAGAG1254.129106E-1019.243
CTTATAC12450.018.57429737
GTATCAA7650.018.1372552
GTATTAG2350.016.5319141
GTTTCGT803.38239E-416.187524
TTAGACC803.38239E-416.18754
ACGCGCG700.00259239315.85714321
TCTTATA21900.015.79680337
TATACAC4150.015.60240937
TGATCGG855.3638767E-415.23529310
TTTATAC750.00410424914.7999993
AACGCCC1405.9936065E-714.53571512
TATTAGG1551.2097189E-714.3225812
CCAAACG1551.2097189E-714.3225819
CGACGTT1304.4432327E-614.23076914
GAATTAG1051.6555232E-414.0952381
Produced by FastQC (version 0.11.5)