##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632273.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1097347
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.859101086529606	34.0	31.0	34.0	31.0	34.0
2	33.032146622718244	34.0	33.0	34.0	31.0	34.0
3	33.0409332690571	34.0	33.0	34.0	31.0	34.0
4	36.4668878668279	37.0	37.0	37.0	35.0	37.0
5	36.38556445682177	37.0	37.0	37.0	35.0	37.0
6	36.414034940634096	37.0	37.0	37.0	35.0	37.0
7	36.40898457826011	37.0	37.0	37.0	35.0	37.0
8	36.38840403263507	37.0	37.0	37.0	35.0	37.0
9	38.19899721783538	39.0	39.0	39.0	37.0	39.0
10	38.1322371136933	39.0	38.0	39.0	37.0	39.0
11	38.236612484473916	39.0	39.0	39.0	37.0	39.0
12	38.20731363916792	39.0	39.0	39.0	37.0	39.0
13	38.232087024432566	39.0	39.0	39.0	37.0	39.0
14	39.67302138703619	41.0	39.0	41.0	38.0	41.0
15	39.69537530061138	41.0	39.0	41.0	38.0	41.0
16	39.58224062215507	41.0	39.0	41.0	37.0	41.0
17	39.62497368653671	41.0	39.0	41.0	37.0	41.0
18	39.64381731576247	41.0	39.0	41.0	37.0	41.0
19	39.69528963946682	41.0	40.0	41.0	38.0	41.0
20	39.67146946225761	41.0	39.0	41.0	37.0	41.0
21	39.65257935730448	41.0	39.0	41.0	37.0	41.0
22	39.63661540059799	41.0	39.0	41.0	37.0	41.0
23	39.601674766505035	41.0	39.0	41.0	37.0	41.0
24	39.55413374256275	41.0	39.0	41.0	37.0	41.0
25	39.49747254059108	41.0	39.0	41.0	37.0	41.0
26	39.423202505679605	41.0	39.0	41.0	37.0	41.0
27	39.37095376394158	40.0	39.0	41.0	37.0	41.0
28	39.31489127869307	40.0	39.0	41.0	36.0	41.0
29	39.18804170421936	40.0	39.0	41.0	36.0	41.0
30	39.15111901704748	40.0	39.0	41.0	36.0	41.0
31	39.09845381634069	40.0	39.0	41.0	36.0	41.0
32	39.05197626639522	40.0	39.0	41.0	35.0	41.0
33	38.92908715292428	40.0	39.0	41.0	35.0	41.0
34	38.94054114149854	40.0	38.0	41.0	35.0	41.0
35	38.92192351188822	40.0	38.0	41.0	35.0	41.0
36	38.90570348303682	40.0	38.0	41.0	35.0	41.0
37	38.87659509708415	40.0	38.0	41.0	35.0	41.0
38	38.83225543059761	40.0	38.0	41.0	35.0	41.0
39	38.66156466459561	40.0	38.0	41.0	35.0	41.0
40	38.678031652704206	40.0	38.0	41.0	35.0	41.0
41	38.69154971034686	40.0	38.0	41.0	35.0	41.0
42	38.58065042324807	40.0	38.0	41.0	35.0	41.0
43	37.765459786193425	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	3.0
17	0.0
18	9.0
19	18.0
20	41.0
21	79.0
22	175.0
23	279.0
24	423.0
25	723.0
26	1049.0
27	1518.0
28	2250.0
29	3218.0
30	4419.0
31	6185.0
32	9060.0
33	13426.0
34	21160.0
35	34038.0
36	57950.0
37	111052.0
38	276302.0
39	553969.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.075708959882334	17.40680021907382	12.011697302676364	27.505793518367483
2	19.470413643086463	19.244414027650325	33.346152128725	27.939020200538206
3	20.149232649289605	20.881453177527256	28.596332791724038	30.372981381459102
4	15.496374437620917	14.862573096750618	33.11377349188543	36.52727897374304
5	16.87369628750067	33.849001273070414	32.93944394981715	16.337858489611765
6	36.698327876232405	33.173189519814606	14.922535897942948	15.20594670601004
7	31.86312078130254	28.37853477523518	20.008256276273595	19.750088167188686
8	28.793080037581547	31.886814289372463	18.946513728109704	20.373591944936287
9	27.85363244260931	13.638347760553406	18.163352157521732	40.34466763931555
10	18.16271425538139	25.15940718842809	31.717314577795356	24.960563978395168
11	38.42913864073989	20.076967449676356	20.085077919746443	21.408815989837308
12	23.539591396340445	23.62990011363771	27.455034733771544	25.3754737562503
13	32.569916352803624	18.11523611036436	22.333956351090404	26.980891185741612
14	24.291860277560335	18.617720739200998	23.358700575114344	33.73171840812432
15	27.151666701599403	25.36845683270652	20.464811951005473	27.015064514688607
16	27.223749643458266	23.99423336465129	22.10686318912796	26.67515380276248
17	25.862375347087113	24.398025419489002	23.58552035044521	26.154078882978677
18	26.018296855962607	23.577865524761084	23.985302734686474	26.418534884589835
19	26.75525608581424	23.51580675939334	24.06841227068557	25.66052488410685
20	26.910721950303778	23.52619545139322	23.54833976855088	26.01474282975212
21	27.24862782693168	23.471244738446455	22.934860167294392	26.345267267327472
22	27.696799644961896	23.24788786044888	22.982885085574573	26.072427409014654
23	26.971231524759258	23.35788041521962	23.135070310485197	26.53581774953593
24	26.55386126721994	23.404447271464722	22.942150477469752	27.099540983845582
25	26.867526862514772	22.727268585051036	23.45465928279751	26.950545269636677
26	26.283117373082533	23.103813105608346	23.651679915286596	26.96138960602252
27	26.188434469680054	23.42804965065745	23.497398726200554	26.886117153461942
28	26.42810341669499	23.3762884484124	23.65641861690058	26.53918951799203
29	26.349550324555498	23.63308962433943	23.80058450061831	26.216775550486766
30	26.09256689087408	23.90027949226635	23.207153252344064	26.80000036451551
31	26.419172786730176	23.456755246972925	23.34840301199165	26.77566895430525
32	25.67674582424703	23.585611479322402	23.77488615725017	26.962756539180404
33	25.492300976810434	23.4001642142367	24.34754002152464	26.759994787428226
34	26.173398204943375	22.576905937684252	24.440218089628896	26.809477767743477
35	25.644668459475444	22.891847337259776	25.049323504780162	26.41416069848462
36	25.55736699512552	22.52714957073742	24.765912696713073	27.14957073742399
37	25.07894038986756	21.588248749028338	25.724406227018438	27.608404634085666
38	24.440309218506087	21.772237952078967	26.654649805394282	27.132803024020664
39	24.213671700929606	21.407904701065387	27.425782364192912	26.95264123381209
40	23.986578538967162	20.93020712682497	28.48096363319898	26.602250701008888
41	23.352868326974058	20.780208994966955	28.940162045369423	26.926760632689568
42	22.54391728414075	20.793149295528217	29.51026430108252	27.15266911924851
43	21.889065172639103	20.27772436613031	30.054303697918705	27.778906763311877
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	17.0
1	17.5
2	18.0
3	42.5
4	67.0
5	67.0
6	98.5
7	130.0
8	130.5
9	131.0
10	174.0
11	217.0
12	217.0
13	409.5
14	602.0
15	959.0
16	1316.0
17	1436.5
18	1557.0
19	1557.0
20	1948.0
21	2339.0
22	2632.0
23	2925.0
24	3759.0
25	4593.0
26	4593.0
27	6015.5
28	7438.0
29	9721.0
30	12004.0
31	14876.0
32	17748.0
33	17748.0
34	21857.5
35	25967.0
36	30464.5
37	34962.0
38	40054.0
39	45146.0
40	45146.0
41	50553.5
42	55961.0
43	61375.5
44	66790.0
45	70953.5
46	75117.0
47	75117.0
48	78704.0
49	82291.0
50	84533.0
51	86775.0
52	87975.5
53	89176.0
54	89176.0
55	88242.5
56	87309.0
57	85069.0
58	82829.0
59	79725.0
60	76621.0
61	76621.0
62	71082.0
63	65543.0
64	58567.5
65	51592.0
66	45419.5
67	39247.0
68	39247.0
69	33785.0
70	28323.0
71	24239.5
72	20156.0
73	16377.5
74	12599.0
75	12599.0
76	10405.0
77	8211.0
78	6774.5
79	5338.0
80	4290.0
81	3242.0
82	3242.0
83	2444.0
84	1646.0
85	1234.0
86	822.0
87	605.0
88	388.0
89	388.0
90	268.5
91	149.0
92	88.0
93	27.0
94	18.5
95	10.0
96	10.0
97	8.5
98	7.0
99	4.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1097347.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.81818015620559
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.43602209415371	62.79495986899904
2	7.476567455067126	10.739069368760685
3	2.057043686928663	4.4319940229108425
4	0.8810880131525832	2.531125506482618
5	0.5008212263825876	1.7984034531198247
6	0.32235449513853404	1.389054792361316
7	0.22561303414359835	1.1342182272199175
8	0.16084193769827965	0.9241100206630594
9	0.12044115561759774	0.7784878151129604
>10	0.7636647250174612	10.279362079665407
>50	0.047143458789883595	2.2815585292496596
>100	0.008144212053507282	0.8126483583692939
>500	2.545058564232096E-4	0.10500795708537883
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	9.112887719199123E-5	0.0	0.0	0.0
3	0.0	9.112887719199123E-5	0.0	0.0	0.0
4	0.0	9.112887719199123E-5	0.0	0.0	0.0
5	0.0	9.112887719199123E-5	0.0	0.0	0.0
6	0.0	9.112887719199123E-5	0.0	0.0	0.0
7	0.0	9.112887719199123E-5	0.0	0.0	0.0
8	0.0	9.112887719199123E-5	0.0	0.0	0.0
9	0.0	9.112887719199123E-5	0.0	2.733866315759737E-4	0.0
10	0.0	1.8225775438398246E-4	0.0	2.733866315759737E-4	9.112887719199123E-5
11	0.0	1.8225775438398246E-4	0.0	3.645155087679649E-4	9.112887719199123E-5
12	0.0	1.8225775438398246E-4	0.0	4.5564438595995616E-4	9.112887719199123E-5
13	0.0	1.8225775438398246E-4	0.0	4.5564438595995616E-4	9.112887719199123E-5
14	0.0	1.8225775438398246E-4	0.0	4.5564438595995616E-4	9.112887719199123E-5
15	0.0	1.8225775438398246E-4	0.0	4.5564438595995616E-4	9.112887719199123E-5
16	0.0	1.8225775438398246E-4	0.0	7.290310175359298E-4	9.112887719199123E-5
17	0.0	1.8225775438398246E-4	0.0	8.201598947279211E-4	9.112887719199123E-5
18	0.0	1.8225775438398246E-4	0.0	9.112887719199123E-4	9.112887719199123E-5
19	0.0	1.8225775438398246E-4	0.0	0.0010024176491119036	9.112887719199123E-5
20	0.0	1.8225775438398246E-4	0.0	0.0010024176491119036	9.112887719199123E-5
21	0.0	1.8225775438398246E-4	0.0	0.0012758042806878772	9.112887719199123E-5
22	0.0	1.8225775438398246E-4	0.0	0.0018225775438398247	9.112887719199123E-5
23	0.0	2.733866315759737E-4	0.0	0.0025516085613757544	9.112887719199123E-5
24	0.0	2.733866315759737E-4	0.0	0.0038274128420636316	9.112887719199123E-5
25	0.0	2.733866315759737E-4	0.0	0.005012088245559518	9.112887719199123E-5
26	0.0	2.733866315759737E-4	0.0	0.008748372210431158	9.112887719199123E-5
27	0.0	3.645155087679649E-4	0.0	0.032077364771580914	9.112887719199123E-5
28	0.0	4.5564438595995616E-4	0.0	0.11855866922678059	9.112887719199123E-5
29	0.0	7.290310175359298E-4	0.0	0.24458990638330447	9.112887719199123E-5
30	0.0	7.290310175359298E-4	0.0	0.40998881848676855	9.112887719199123E-5
31	0.0	7.290310175359298E-4	0.0	0.9605894944807796	9.112887719199123E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	475	0.0	29.210526	1
TATTAGA	115	1.3460522E-10	20.913044	2
CGTTTCG	55	5.142889E-4	20.181818	23
TTAGAGT	75	9.265146E-6	19.733332	4
ATTAGAG	125	4.129106E-10	19.24	3
CTTATAC	1245	0.0	18.574297	37
GTATCAA	765	0.0	18.137255	2
GTATTAG	235	0.0	16.531914	1
GTTTCGT	80	3.38239E-4	16.1875	24
TTAGACC	80	3.38239E-4	16.1875	4
ACGCGCG	70	0.002592393	15.857143	21
TCTTATA	2190	0.0	15.796803	37
TATACAC	415	0.0	15.602409	37
TGATCGG	85	5.3638767E-4	15.235293	10
TTTATAC	75	0.004104249	14.799999	3
AACGCCC	140	5.9936065E-7	14.535715	12
TATTAGG	155	1.2097189E-7	14.322581	2
CCAAACG	155	1.2097189E-7	14.322581	9
CGACGTT	130	4.4432327E-6	14.230769	14
GAATTAG	105	1.6555232E-4	14.095238	1
>>END_MODULE