FastQCFastQC Report
Fri 10 Feb 2017
ERR1632272.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632272.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141001
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCCG200.001836465637.05
GGTATCA801.8189894E-1230.06251
ATCTGCG250.00548020629.67
GGTCGGG451.3151862E-424.6666661
CTTATAC903.756213E-922.6111137
CTACCAT502.6858452E-422.21
CTGCGGT450.00380943320.5555559
TTGCGCG450.00380943320.55555518
TTTGCGC450.00380943320.55555517
GTCGGGA450.00380943320.5555552
TCTTATA2050.019.8536637
AATTTGC500.007005060618.515
GGGTAAT609.1821444E-418.511
ACGGGGA500.007005060618.522
ATTTGCG500.007005060618.516
GGGAGTG752.051108E-417.2666665
GTATCAA1452.904926E-916.5862062
GGAGTGG1101.4339137E-515.1363646
CTCTTAT2700.015.07407536
GCCACTG908.210726E-414.38888836