FastQCFastQC Report
Fri 10 Feb 2017
ERR1632271.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632271.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40553
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT4291.0578748797869455RNA PCR Primer, Index 29 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGT3500.8630680837422633No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2820.6953862846151949No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2820.6953862846151949No Hit
CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC2650.6534658348334279RNA PCR Primer, Index 29 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1890.4660567652208221No Hit
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC1860.45865903878874564No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATG1670.4118067713855942No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1040.2564545163119868No Hit
CTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTC1000.24659088106921806RNA PCR Primer, Index 29 (95% over 24bp)
CACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCG530.13069316696668556No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT500.12329544053460903No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC430.10603407885976376No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATAG200.001821077237.01
GGTATCA1050.029.952381
AGTAGGC250.0054348129.66
GCCTAAA250.0054348129.611
GGCCTAA250.0054348129.610
ATAGTAG250.0054348129.64
CATAGTA250.0054348129.63
CCATAGT250.0054348129.62
GCCGTCT706.200935E-926.4285736
CCGTCTT751.2925739E-824.66666437
TATACAC603.6135192E-521.5833343
CAGCCAC450.003763465720.55555520
AGCCACC450.003763465720.55555521
AGTACTG450.003763465720.5555555
GCCACCA450.003763465720.55555522
GTATCAA1550.020.2903232
TTATACA656.694718E-519.9230772
TACACAT801.5549596E-518.55
ATACACA852.6198171E-517.4117644
TATGCCG1107.361832E-716.81818233
Produced by FastQC (version 0.11.5)